Detection of laboratory biomarkes in haematological and pulmonology patients at high risk for aspergillosis
Introduction. During the past decades a dramatic increase in the incidence of invasive fungal diseases, especially invasive aspergillosis has been observed. Undiagnosed and diagnosed late invasive aspergillosis is followed by lethality of up to 90%. Detection of early laboratory biomarkers (gala...
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| Format: | Article |
| Language: | English |
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Serbian Medical Society
2012-01-01
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| Series: | Srpski Arhiv za Celokupno Lekarstvo |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0370-8179/2012/0370-81791206290R.pdf |
| _version_ | 1818389863666286592 |
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| author | Ratkov Eleonora Vidović Ana Minić Predrag Janić Dragana Šipetić-Grujičić Sandra Džamić Aleksandar Arsić-Arsenijević Valentina |
| author_facet | Ratkov Eleonora Vidović Ana Minić Predrag Janić Dragana Šipetić-Grujičić Sandra Džamić Aleksandar Arsić-Arsenijević Valentina |
| author_sort | Ratkov Eleonora |
| collection | DOAJ |
| description | Introduction. During the past decades a dramatic increase in the incidence of invasive fungal diseases, especially invasive aspergillosis has been observed. Undiagnosed and diagnosed late invasive aspergillosis is followed by lethality of up to 90%. Detection of early laboratory biomarkers (galactomannan and anti-Aspergillus antibodies) contributes to early diagnosis and is used for screening, as well as for monitoring therapy of aspergillosis. Objective. The aim was to evaluate usefulness of “non-culture” methods (galactomannan and anti-Aspergillus antibodies IgA, IgM and IgG) for early diagnosis of aspergillosis in high-risk patients. Methods. Prospective two-year study involved 262 high-risk patients for aspergillosis. In pulmonology and haematology patients (adults and children) blood samples were tested on galactomannan and anti-Aspergillus antibodies. Results. Early laboratory biomarkers were statistically significantly higher in pulmonology patients (p=0.00033). However, in haematological patients galactomannan was a more frequently positive finding, while in pulmonology patients it was the finding of anti-Aspergillus antibodies. It is interesting that, despite the possible immunosuppression, in about 1/3 of haematological patients anti-Aspergillus antibodies were confirmed. Conclusion. Early diagnosis and treatment of aspergillosis represent both clinical and laboratory problem. Nowadays, the application of new “non-culture” methods is of particular importance for the outcome of aspergillosis. Clinical features, laboratory findings of biomarkers and their correct interpretation significantly increase the possibility of timely implementation of appropriate therapy. In this regard, the new organization of reference laboratory for medical mycology has significantly improved the outcome of aspergillosis in high-risk patients in our country. However, further investigations, implementation of European standards and introduction of new diagnostic methods are necessary in this field. |
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| format | Article |
| id | doaj.art-e9697fd208f344b9a8cd34b3d8d77206 |
| institution | Directory Open Access Journal |
| issn | 0370-8179 |
| language | English |
| last_indexed | 2024-12-14T04:48:30Z |
| publishDate | 2012-01-01 |
| publisher | Serbian Medical Society |
| record_format | Article |
| series | Srpski Arhiv za Celokupno Lekarstvo |
| spelling | doaj.art-e9697fd208f344b9a8cd34b3d8d772062022-12-21T23:16:36ZengSerbian Medical SocietySrpski Arhiv za Celokupno Lekarstvo0370-81792012-01-011405-629029810.2298/SARH1206290RDetection of laboratory biomarkes in haematological and pulmonology patients at high risk for aspergillosisRatkov EleonoraVidović AnaMinić PredragJanić DraganaŠipetić-Grujičić SandraDžamić AleksandarArsić-Arsenijević ValentinaIntroduction. During the past decades a dramatic increase in the incidence of invasive fungal diseases, especially invasive aspergillosis has been observed. Undiagnosed and diagnosed late invasive aspergillosis is followed by lethality of up to 90%. Detection of early laboratory biomarkers (galactomannan and anti-Aspergillus antibodies) contributes to early diagnosis and is used for screening, as well as for monitoring therapy of aspergillosis. Objective. The aim was to evaluate usefulness of “non-culture” methods (galactomannan and anti-Aspergillus antibodies IgA, IgM and IgG) for early diagnosis of aspergillosis in high-risk patients. Methods. Prospective two-year study involved 262 high-risk patients for aspergillosis. In pulmonology and haematology patients (adults and children) blood samples were tested on galactomannan and anti-Aspergillus antibodies. Results. Early laboratory biomarkers were statistically significantly higher in pulmonology patients (p=0.00033). However, in haematological patients galactomannan was a more frequently positive finding, while in pulmonology patients it was the finding of anti-Aspergillus antibodies. It is interesting that, despite the possible immunosuppression, in about 1/3 of haematological patients anti-Aspergillus antibodies were confirmed. Conclusion. Early diagnosis and treatment of aspergillosis represent both clinical and laboratory problem. Nowadays, the application of new “non-culture” methods is of particular importance for the outcome of aspergillosis. Clinical features, laboratory findings of biomarkers and their correct interpretation significantly increase the possibility of timely implementation of appropriate therapy. In this regard, the new organization of reference laboratory for medical mycology has significantly improved the outcome of aspergillosis in high-risk patients in our country. However, further investigations, implementation of European standards and introduction of new diagnostic methods are necessary in this field.http://www.doiserbia.nb.rs/img/doi/0370-8179/2012/0370-81791206290R.pdfaspergillosisearly laboratory diagnosisgalactomannananti-Aspergillus antibodieshigh-risk patients |
| spellingShingle | Ratkov Eleonora Vidović Ana Minić Predrag Janić Dragana Šipetić-Grujičić Sandra Džamić Aleksandar Arsić-Arsenijević Valentina Detection of laboratory biomarkes in haematological and pulmonology patients at high risk for aspergillosis Srpski Arhiv za Celokupno Lekarstvo aspergillosis early laboratory diagnosis galactomannan anti-Aspergillus antibodies high-risk patients |
| title | Detection of laboratory biomarkes in haematological and pulmonology patients at high risk for aspergillosis |
| title_full | Detection of laboratory biomarkes in haematological and pulmonology patients at high risk for aspergillosis |
| title_fullStr | Detection of laboratory biomarkes in haematological and pulmonology patients at high risk for aspergillosis |
| title_full_unstemmed | Detection of laboratory biomarkes in haematological and pulmonology patients at high risk for aspergillosis |
| title_short | Detection of laboratory biomarkes in haematological and pulmonology patients at high risk for aspergillosis |
| title_sort | detection of laboratory biomarkes in haematological and pulmonology patients at high risk for aspergillosis |
| topic | aspergillosis early laboratory diagnosis galactomannan anti-Aspergillus antibodies high-risk patients |
| url | http://www.doiserbia.nb.rs/img/doi/0370-8179/2012/0370-81791206290R.pdf |
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