Mixed mode monolithic sorbent in pipette tip for extraction of ractopamine and clenbuterol prior to analysis by HPLC-UV and UHPLC-Q ExactiveTM Plus Orbitrap MS
Abstract This work developed a fast and simple method for the quantification of two important β-agonists, ractopamine (RAC) and clenbuterol (CLEN) in animal urines. The method was based on micro-extraction by a mixed mode monolithic material and analysis by HPLC-UV and UHPLC-Q ExactiveTM Plus Orbitr...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2021-05-01
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| Series: | Journal of Analytical Science and Technology |
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| Online Access: | https://doi.org/10.1186/s40543-021-00275-5 |
| _version_ | 1819082129556897792 |
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| author | Kesara Ar-sanork Chanpen Karuwan Nakin Surapanich Prapin Wilairat Duangjai Nacapricha Patcharin Chaisuwan |
| author_facet | Kesara Ar-sanork Chanpen Karuwan Nakin Surapanich Prapin Wilairat Duangjai Nacapricha Patcharin Chaisuwan |
| author_sort | Kesara Ar-sanork |
| collection | DOAJ |
| description | Abstract This work developed a fast and simple method for the quantification of two important β-agonists, ractopamine (RAC) and clenbuterol (CLEN) in animal urines. The method was based on micro-extraction by a mixed mode monolithic material and analysis by HPLC-UV and UHPLC-Q ExactiveTM Plus Orbitrap mass spectrometer. A mixed functional monolith with nonpolar ester-based structure containing polar carboxylic groups was thermally synthesized in situ in the pipette tips. The material combines both hydrophobic and ionic interactions. Parameters including conditions and composition of reagents for the in-pipette tip monolith synthesis, as well as the extraction process, were investigated and optimized. The procedure for extraction is 800 μL washing solvent of 10:90 (v/v) ACN:water and 150 μL eluting solvent of 30:70 (v/v) ACN:200 mM acetate buffer pH 4.0. Extraction efficiencies of 92% and 100% for RAC and CLEN, respectively, were achieved within 5 min with total organic solvent consumption of 395 μL. The extracts of spiked cattle and swine urines were analyzed by HPLC-UV and UHPLC-Q ExactiveTM Plus Orbitrap mass spectrometer. Good recovery with acceptable precision was observed. The mass spectrometry data confirmed efficient matrix removal by the synthesized extraction sorbent allowing routine analysis by the HPLC-UV method. |
| first_indexed | 2024-12-21T20:11:46Z |
| format | Article |
| id | doaj.art-e969fad59c0548bfbbba37d97ca54a27 |
| institution | Directory Open Access Journal |
| issn | 2093-3371 |
| language | English |
| last_indexed | 2024-12-21T20:11:46Z |
| publishDate | 2021-05-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Journal of Analytical Science and Technology |
| spelling | doaj.art-e969fad59c0548bfbbba37d97ca54a272022-12-21T18:51:45ZengSpringerOpenJournal of Analytical Science and Technology2093-33712021-05-0112111010.1186/s40543-021-00275-5Mixed mode monolithic sorbent in pipette tip for extraction of ractopamine and clenbuterol prior to analysis by HPLC-UV and UHPLC-Q ExactiveTM Plus Orbitrap MSKesara Ar-sanork0Chanpen Karuwan1Nakin Surapanich2Prapin Wilairat3Duangjai Nacapricha4Patcharin Chaisuwan5School of Chemistry, Institute of Science, Suranaree University of TechnologyGraphene and Printed Electronics for Dual-Use Applications Research Division (GPERD), National Science and Technology Development Agency (NSTDA)Department of Chemistry, Faculty of Science and Technology, Rajanagarindra Rajabhat UniversityFlow Innovation-Research for Science Technology Laboratories (FIRST Labs.)Flow Innovation-Research for Science Technology Laboratories (FIRST Labs.)School of Chemistry, Institute of Science, Suranaree University of TechnologyAbstract This work developed a fast and simple method for the quantification of two important β-agonists, ractopamine (RAC) and clenbuterol (CLEN) in animal urines. The method was based on micro-extraction by a mixed mode monolithic material and analysis by HPLC-UV and UHPLC-Q ExactiveTM Plus Orbitrap mass spectrometer. A mixed functional monolith with nonpolar ester-based structure containing polar carboxylic groups was thermally synthesized in situ in the pipette tips. The material combines both hydrophobic and ionic interactions. Parameters including conditions and composition of reagents for the in-pipette tip monolith synthesis, as well as the extraction process, were investigated and optimized. The procedure for extraction is 800 μL washing solvent of 10:90 (v/v) ACN:water and 150 μL eluting solvent of 30:70 (v/v) ACN:200 mM acetate buffer pH 4.0. Extraction efficiencies of 92% and 100% for RAC and CLEN, respectively, were achieved within 5 min with total organic solvent consumption of 395 μL. The extracts of spiked cattle and swine urines were analyzed by HPLC-UV and UHPLC-Q ExactiveTM Plus Orbitrap mass spectrometer. Good recovery with acceptable precision was observed. The mass spectrometry data confirmed efficient matrix removal by the synthesized extraction sorbent allowing routine analysis by the HPLC-UV method.https://doi.org/10.1186/s40543-021-00275-5Micro-solid phase extractionMonolithRactopamineClenbuterolHPLC analysis |
| spellingShingle | Kesara Ar-sanork Chanpen Karuwan Nakin Surapanich Prapin Wilairat Duangjai Nacapricha Patcharin Chaisuwan Mixed mode monolithic sorbent in pipette tip for extraction of ractopamine and clenbuterol prior to analysis by HPLC-UV and UHPLC-Q ExactiveTM Plus Orbitrap MS Journal of Analytical Science and Technology Micro-solid phase extraction Monolith Ractopamine Clenbuterol HPLC analysis |
| title | Mixed mode monolithic sorbent in pipette tip for extraction of ractopamine and clenbuterol prior to analysis by HPLC-UV and UHPLC-Q ExactiveTM Plus Orbitrap MS |
| title_full | Mixed mode monolithic sorbent in pipette tip for extraction of ractopamine and clenbuterol prior to analysis by HPLC-UV and UHPLC-Q ExactiveTM Plus Orbitrap MS |
| title_fullStr | Mixed mode monolithic sorbent in pipette tip for extraction of ractopamine and clenbuterol prior to analysis by HPLC-UV and UHPLC-Q ExactiveTM Plus Orbitrap MS |
| title_full_unstemmed | Mixed mode monolithic sorbent in pipette tip for extraction of ractopamine and clenbuterol prior to analysis by HPLC-UV and UHPLC-Q ExactiveTM Plus Orbitrap MS |
| title_short | Mixed mode monolithic sorbent in pipette tip for extraction of ractopamine and clenbuterol prior to analysis by HPLC-UV and UHPLC-Q ExactiveTM Plus Orbitrap MS |
| title_sort | mixed mode monolithic sorbent in pipette tip for extraction of ractopamine and clenbuterol prior to analysis by hplc uv and uhplc q exactivetm plus orbitrap ms |
| topic | Micro-solid phase extraction Monolith Ractopamine Clenbuterol HPLC analysis |
| url | https://doi.org/10.1186/s40543-021-00275-5 |
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