Influence of enzyme-aided extraction and ultrasonication on the phenolics content and antioxidant activity of Paeonia officinalis L. petals
Peony is a less utilized herbaceous plant of ornamental and medicinal relevance. The petals are rich in bioactive phenolics and hence, the development of efficient extraction is required for maximum recovery and bioactivity. In this study, the optimal conditions for the ultrasound-assisted extractio...
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| Format: | Article |
| Language: | English |
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Serbian Chemical Society
2020-01-01
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| Series: | Journal of the Serbian Chemical Society |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0352-5139/2020/0352-51391900120O.pdf |
| _version_ | 1811286156546932736 |
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| author | Oancea Simona Perju Mirabela Olosutean Horea |
| author_facet | Oancea Simona Perju Mirabela Olosutean Horea |
| author_sort | Oancea Simona |
| collection | DOAJ |
| description | Peony is a less utilized herbaceous plant of ornamental and medicinal relevance. The petals are rich in bioactive phenolics and hence, the development of efficient extraction is required for maximum recovery and bioactivity. In this study, the optimal conditions for the ultrasound-assisted extraction of peony phenolics were first investigated by comparing to conventional extraction and testing the data fit by mathematical models (linear, two factor interactions, quadratic) followed by investigation of cellulase pre-treatment combined with ultrasonication. High amounts of flavonoids, phenolics and tannins were extracted in 30 min under ultrasound conditions of 150 W, 40 kHz, 50 % amplitude, in 70 % ethanol with 50/1 solvent/solid ratio. A further cellulase pre-treatment at 40 °C for different incubation times combined with ultrasonication facilitated the extraction of anthocyanins (720.48 mg/100 g DW), phenolics (3985.50 mg/100 g DW), flavonoids (2369.28 mg/100 g DW) and tannins (8917.81 mg/100 g DW). Good antioxidant activities by FRAP (350.03 mg ascorbic acid/100 g DW) and DPPH (inhibition values >80 %) were registered showing great potential for developing high-value bioingredients. The FTIR analysis revealed the presence of characteristic functional groups. The hereby- -combined extraction methods could be extended to other peony species. |
| first_indexed | 2024-04-13T02:55:27Z |
| format | Article |
| id | doaj.art-e96d0251c7f7492588bd5dfbff6bf78f |
| institution | Directory Open Access Journal |
| issn | 0352-5139 1820-7421 |
| language | English |
| last_indexed | 2024-04-13T02:55:27Z |
| publishDate | 2020-01-01 |
| publisher | Serbian Chemical Society |
| record_format | Article |
| series | Journal of the Serbian Chemical Society |
| spelling | doaj.art-e96d0251c7f7492588bd5dfbff6bf78f2022-12-22T03:05:42ZengSerbian Chemical SocietyJournal of the Serbian Chemical Society0352-51391820-74212020-01-0185784585610.2298/JSC190807120O0352-51391900120OInfluence of enzyme-aided extraction and ultrasonication on the phenolics content and antioxidant activity of Paeonia officinalis L. petalsOancea Simona0Perju Mirabela1Olosutean Horea2Department of Agricultural Sciences and Food Products Engineering, ”Lucian Blaga” University of Sibiu, Sibiu, Romania,Department of Environmental Sciences, ”Lucian Blaga” University of Sibiu, Sibiu, RomaniaDepartment of Environmental Sciences, ”Lucian Blaga” University of Sibiu, Sibiu, RomaniaPeony is a less utilized herbaceous plant of ornamental and medicinal relevance. The petals are rich in bioactive phenolics and hence, the development of efficient extraction is required for maximum recovery and bioactivity. In this study, the optimal conditions for the ultrasound-assisted extraction of peony phenolics were first investigated by comparing to conventional extraction and testing the data fit by mathematical models (linear, two factor interactions, quadratic) followed by investigation of cellulase pre-treatment combined with ultrasonication. High amounts of flavonoids, phenolics and tannins were extracted in 30 min under ultrasound conditions of 150 W, 40 kHz, 50 % amplitude, in 70 % ethanol with 50/1 solvent/solid ratio. A further cellulase pre-treatment at 40 °C for different incubation times combined with ultrasonication facilitated the extraction of anthocyanins (720.48 mg/100 g DW), phenolics (3985.50 mg/100 g DW), flavonoids (2369.28 mg/100 g DW) and tannins (8917.81 mg/100 g DW). Good antioxidant activities by FRAP (350.03 mg ascorbic acid/100 g DW) and DPPH (inhibition values >80 %) were registered showing great potential for developing high-value bioingredients. The FTIR analysis revealed the presence of characteristic functional groups. The hereby- -combined extraction methods could be extended to other peony species.http://www.doiserbia.nb.rs/img/doi/0352-5139/2020/0352-51391900120O.pdfcellulasedpphfrappeonypolyphenolsultrasounds-assisted extraction |
| spellingShingle | Oancea Simona Perju Mirabela Olosutean Horea Influence of enzyme-aided extraction and ultrasonication on the phenolics content and antioxidant activity of Paeonia officinalis L. petals Journal of the Serbian Chemical Society cellulase dpph frap peony polyphenols ultrasounds-assisted extraction |
| title | Influence of enzyme-aided extraction and ultrasonication on the phenolics content and antioxidant activity of Paeonia officinalis L. petals |
| title_full | Influence of enzyme-aided extraction and ultrasonication on the phenolics content and antioxidant activity of Paeonia officinalis L. petals |
| title_fullStr | Influence of enzyme-aided extraction and ultrasonication on the phenolics content and antioxidant activity of Paeonia officinalis L. petals |
| title_full_unstemmed | Influence of enzyme-aided extraction and ultrasonication on the phenolics content and antioxidant activity of Paeonia officinalis L. petals |
| title_short | Influence of enzyme-aided extraction and ultrasonication on the phenolics content and antioxidant activity of Paeonia officinalis L. petals |
| title_sort | influence of enzyme aided extraction and ultrasonication on the phenolics content and antioxidant activity of paeonia officinalis l petals |
| topic | cellulase dpph frap peony polyphenols ultrasounds-assisted extraction |
| url | http://www.doiserbia.nb.rs/img/doi/0352-5139/2020/0352-51391900120O.pdf |
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