Follicle Development of Xenotransplanted Sheep Ovarian Tissue into Male and Female Immunodeficient Rats
Background This study aimed to assess follicle survival after xenotransplantation of sheep ovarian tissue into male and female immunodeficient rats. We evaluated the effects of gonadotropin treatment on follicular development in the transplanted tissue. Materials and Methods In this experimental stu...
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2015-10-01
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| Series: | International Journal of Fertility and Sterility |
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| Online Access: | http://www.ijfs.ir/article_45324_a70328aeb6ca136645b7feb82a2eeff7.pdf |
| _version_ | 1818188966756614144 |
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| author | Leila Sadat Tahaei Hussein Eimani Ghazaleh Hajmusa Rouhollah Fathi Mojtaba Rezazadeh Valojerdi Abdolhossein Shahverdi Poopak Eftekhari-Yazdi |
| author_facet | Leila Sadat Tahaei Hussein Eimani Ghazaleh Hajmusa Rouhollah Fathi Mojtaba Rezazadeh Valojerdi Abdolhossein Shahverdi Poopak Eftekhari-Yazdi |
| author_sort | Leila Sadat Tahaei |
| collection | DOAJ |
| description | Background This study aimed to assess follicle survival after xenotransplantation of sheep ovarian tissue into male and female immunodeficient rats. We evaluated the effects of gonadotropin treatment on follicular development in the transplanted tissue. Materials and Methods In this experimental study, sheep ovarian cortical strips were transplanted into the neck back muscles of 8 male and 8 female immunodeficient, castrated rats. Fourteen days after surgery, each rat was treated with human menopausal gonadotropin (hMG) for 9 weeks. One day after the last injection, ovarian tissues were removed and fixed for histology assessment. Histology analyses were performed before and after grafting. Estradiol (E2) levels were measured before and after gonadectomy, and at the end of the experiment. The control group consisted of 7 male and 7 female noncastrated/non-grafted rats and the sham group comprised 7 male and 7 female castrated/ non-grafted rats for comparison of serum E2 concentrations. Results The percentage of primordial follicles decreased after transplantation in male (25.97%) and female (24.14%) rats compared to the control group (ovarian tissue nongrafted; 37.51%). Preantral follicles increased in the male (19.5%) and female (19.49%) transplanted rats compared to the control group (11.4%). Differences in antral follicles between male (0.06 ± 0.0%) and female (0.06 ± 0.0%) rats were not noticeable compared to control (1.25 ± 0.0%) rats. We observed a significantly higher percent of mean E2 secretion in grafted males compared to grafted females (P˂0.05). Conclusion Despite significant differences in E2 secretion between xenografted male and female rats, we observed no statistical differences in terms of follicular development. |
| first_indexed | 2024-12-11T23:35:19Z |
| format | Article |
| id | doaj.art-e96e1a7657b44f97afd363a62371e060 |
| institution | Directory Open Access Journal |
| issn | 2008-076X 2008-0778 |
| language | English |
| last_indexed | 2024-12-11T23:35:19Z |
| publishDate | 2015-10-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | International Journal of Fertility and Sterility |
| spelling | doaj.art-e96e1a7657b44f97afd363a62371e0602022-12-22T00:45:54ZengRoyan Institute (ACECR), TehranInternational Journal of Fertility and Sterility2008-076X2008-07782015-10-019335436010.22074/ijfs.2015.455145324Follicle Development of Xenotransplanted Sheep Ovarian Tissue into Male and Female Immunodeficient RatsLeila Sadat Tahaei0Hussein Eimani1Ghazaleh Hajmusa2Rouhollah Fathi3Mojtaba Rezazadeh Valojerdi4Abdolhossein Shahverdi5Poopak Eftekhari-Yazdi6Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, IranDepartment of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran;Department of Anatomy, Faculty of Medicine, Baqiyatallah University, Tehran, IranAnimal Core Facility, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Tehran, IranDepartment of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, IranDepartment of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, IranDepartment of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, IranDepartment of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, IranBackground This study aimed to assess follicle survival after xenotransplantation of sheep ovarian tissue into male and female immunodeficient rats. We evaluated the effects of gonadotropin treatment on follicular development in the transplanted tissue. Materials and Methods In this experimental study, sheep ovarian cortical strips were transplanted into the neck back muscles of 8 male and 8 female immunodeficient, castrated rats. Fourteen days after surgery, each rat was treated with human menopausal gonadotropin (hMG) for 9 weeks. One day after the last injection, ovarian tissues were removed and fixed for histology assessment. Histology analyses were performed before and after grafting. Estradiol (E2) levels were measured before and after gonadectomy, and at the end of the experiment. The control group consisted of 7 male and 7 female noncastrated/non-grafted rats and the sham group comprised 7 male and 7 female castrated/ non-grafted rats for comparison of serum E2 concentrations. Results The percentage of primordial follicles decreased after transplantation in male (25.97%) and female (24.14%) rats compared to the control group (ovarian tissue nongrafted; 37.51%). Preantral follicles increased in the male (19.5%) and female (19.49%) transplanted rats compared to the control group (11.4%). Differences in antral follicles between male (0.06 ± 0.0%) and female (0.06 ± 0.0%) rats were not noticeable compared to control (1.25 ± 0.0%) rats. We observed a significantly higher percent of mean E2 secretion in grafted males compared to grafted females (P˂0.05). Conclusion Despite significant differences in E2 secretion between xenografted male and female rats, we observed no statistical differences in terms of follicular development.http://www.ijfs.ir/article_45324_a70328aeb6ca136645b7feb82a2eeff7.pdffollicleratsheepxenotransplantation |
| spellingShingle | Leila Sadat Tahaei Hussein Eimani Ghazaleh Hajmusa Rouhollah Fathi Mojtaba Rezazadeh Valojerdi Abdolhossein Shahverdi Poopak Eftekhari-Yazdi Follicle Development of Xenotransplanted Sheep Ovarian Tissue into Male and Female Immunodeficient Rats International Journal of Fertility and Sterility follicle rat sheep xenotransplantation |
| title | Follicle Development of Xenotransplanted Sheep Ovarian Tissue into Male and Female Immunodeficient Rats |
| title_full | Follicle Development of Xenotransplanted Sheep Ovarian Tissue into Male and Female Immunodeficient Rats |
| title_fullStr | Follicle Development of Xenotransplanted Sheep Ovarian Tissue into Male and Female Immunodeficient Rats |
| title_full_unstemmed | Follicle Development of Xenotransplanted Sheep Ovarian Tissue into Male and Female Immunodeficient Rats |
| title_short | Follicle Development of Xenotransplanted Sheep Ovarian Tissue into Male and Female Immunodeficient Rats |
| title_sort | follicle development of xenotransplanted sheep ovarian tissue into male and female immunodeficient rats |
| topic | follicle rat sheep xenotransplantation |
| url | http://www.ijfs.ir/article_45324_a70328aeb6ca136645b7feb82a2eeff7.pdf |
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