Cryo-EM structure of a bacteriophage M13 mini variant
Abstract Filamentous bacteriophages package their circular, single stranded DNA genome with the major coat protein pVIII and the minor coat proteins pIII, pVII, pVI, and pIX. Here, we report the cryo-EM structure of a ~500 Å long bacteriophage M13 mini variant. The distal ends of the mini phage are...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-09-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-41151-7 |
| _version_ | 1797376944727130112 |
|---|---|
| author | Qi Jia Ye Xiang |
| author_facet | Qi Jia Ye Xiang |
| author_sort | Qi Jia |
| collection | DOAJ |
| description | Abstract Filamentous bacteriophages package their circular, single stranded DNA genome with the major coat protein pVIII and the minor coat proteins pIII, pVII, pVI, and pIX. Here, we report the cryo-EM structure of a ~500 Å long bacteriophage M13 mini variant. The distal ends of the mini phage are sealed by two cap-like complexes composed of the minor coat proteins. The top cap complex consists of pVII and pIX, both exhibiting a single helix structure. Arg33 of pVII and Glu29 of pIX, located on the inner surface of the cap, play a key role in recognizing the genome packaging signal. The bottom cap complex is formed by the hook-like structures of pIII and pVI, arranged in helix barrels. Most of the inner ssDNA genome adopts a double helix structure with a similar pitch to that of the A-form double-stranded DNA. These findings provide insights into the assembly of filamentous bacteriophages. |
| first_indexed | 2024-03-08T19:45:55Z |
| format | Article |
| id | doaj.art-e96e4403b84c4a45966b5791f61ade12 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-08T19:45:55Z |
| publishDate | 2023-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-e96e4403b84c4a45966b5791f61ade122023-12-24T12:23:00ZengNature PortfolioNature Communications2041-17232023-09-0114111410.1038/s41467-023-41151-7Cryo-EM structure of a bacteriophage M13 mini variantQi Jia0Ye Xiang1Beijing Frontier Research Center for Biological Structure, Center for Infectious Disease Research, SXMU-Tsinghua Collaborative Innovation Center for Frontier Medicine, Department of Basic Medical Sciences, School of Medicine, Tsinghua UniversityBeijing Frontier Research Center for Biological Structure, Center for Infectious Disease Research, SXMU-Tsinghua Collaborative Innovation Center for Frontier Medicine, Department of Basic Medical Sciences, School of Medicine, Tsinghua UniversityAbstract Filamentous bacteriophages package their circular, single stranded DNA genome with the major coat protein pVIII and the minor coat proteins pIII, pVII, pVI, and pIX. Here, we report the cryo-EM structure of a ~500 Å long bacteriophage M13 mini variant. The distal ends of the mini phage are sealed by two cap-like complexes composed of the minor coat proteins. The top cap complex consists of pVII and pIX, both exhibiting a single helix structure. Arg33 of pVII and Glu29 of pIX, located on the inner surface of the cap, play a key role in recognizing the genome packaging signal. The bottom cap complex is formed by the hook-like structures of pIII and pVI, arranged in helix barrels. Most of the inner ssDNA genome adopts a double helix structure with a similar pitch to that of the A-form double-stranded DNA. These findings provide insights into the assembly of filamentous bacteriophages.https://doi.org/10.1038/s41467-023-41151-7 |
| spellingShingle | Qi Jia Ye Xiang Cryo-EM structure of a bacteriophage M13 mini variant Nature Communications |
| title | Cryo-EM structure of a bacteriophage M13 mini variant |
| title_full | Cryo-EM structure of a bacteriophage M13 mini variant |
| title_fullStr | Cryo-EM structure of a bacteriophage M13 mini variant |
| title_full_unstemmed | Cryo-EM structure of a bacteriophage M13 mini variant |
| title_short | Cryo-EM structure of a bacteriophage M13 mini variant |
| title_sort | cryo em structure of a bacteriophage m13 mini variant |
| url | https://doi.org/10.1038/s41467-023-41151-7 |
| work_keys_str_mv | AT qijia cryoemstructureofabacteriophagem13minivariant AT yexiang cryoemstructureofabacteriophagem13minivariant |