Differential Response of <i>Phaeodactylum</i> <i>tricornutum</i> and <i>Cylindrotheca</i> <i>fusiformis</i> to High Concentrations of Cu<sup>2+</sup> and Zn<sup>2+</sup>
Diatoms can be used as biosensors to assess aquatic environment quality, because they are widely distributed in almost all aquatic environments and show varied sensitivities toward heavy metal ions. The marine planktonic diatoms <i>Phaeodactylum tricornutum</i> (<i>P. tricornutum&l...
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MDPI AG
2022-10-01
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| Online Access: | https://www.mdpi.com/2073-4441/14/20/3305 |
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| author | Aiyou Huang Yujue Wang Jiawen Duan Shiyi Guo Zhenyu Xie |
| author_facet | Aiyou Huang Yujue Wang Jiawen Duan Shiyi Guo Zhenyu Xie |
| author_sort | Aiyou Huang |
| collection | DOAJ |
| description | Diatoms can be used as biosensors to assess aquatic environment quality, because they are widely distributed in almost all aquatic environments and show varied sensitivities toward heavy metal ions. The marine planktonic diatoms <i>Phaeodactylum tricornutum</i> (<i>P. tricornutum</i>) and <i>Cylindrotheca fusiformis</i> (<i>C. fusiformis</i>) are typical representatives of planktonic diatoms and benthic diatoms, respectively. <i>C. fusiformis</i> is very sensitive to changes in the concentration of heavy metal ions, and can be used as an indicator of the quality of the sedimental environment, while <i>P. tricornutum</i> can tolerate higher concentrations of heavy metal ions. To explore the potential difference in responses to heavy metal ions between planktonic and benthic diatoms, we compared the transcriptome of <i>P. tricornutum</i> and <i>C. fusiformis</i> under Cu<sup>2+</sup> and Zn<sup>2+</sup> treatment. The results indicated that <i>P. tricornutum</i> has several genes involved in ion transmembrane transport and ion homeostasis, which are significantly downregulated under Cu<sup>2+</sup> and Zn<sup>2+</sup> treatment. However, this enrichment of ion transmembrane transport- and ion homeostasis-related genes was not observed in <i>C. fusiformis</i> under Cu<sup>2+</sup> and Zn<sup>2+</sup> treatment. Additionally, genes related to heavy metal ion stress response such as peroxiredoxin, peroxidase, catalase, glutathione metabolism, phytochelatin, oxidative stress and disulfide reductase, were upregulated in <i>P. tricornutum</i> under Cu<sup>2+</sup> and Zn<sup>2+</sup> treatment, whereas most of them were downregulated in <i>C. fusiformis</i> under Cu<sup>2+</sup> and Zn<sup>2+</sup> treatment. This difference in gene expression may be responsible for the difference in sensitivity to heavy metals between <i>P. tricornutum</i> and <i>C. fusiformis</i>. |
| first_indexed | 2024-03-09T19:23:25Z |
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| id | doaj.art-e96fd69f8aa346f18cbbc91c5e698984 |
| institution | Directory Open Access Journal |
| issn | 2073-4441 |
| language | English |
| last_indexed | 2024-03-09T19:23:25Z |
| publishDate | 2022-10-01 |
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| spelling | doaj.art-e96fd69f8aa346f18cbbc91c5e6989842023-11-24T03:13:15ZengMDPI AGWater2073-44412022-10-011420330510.3390/w14203305Differential Response of <i>Phaeodactylum</i> <i>tricornutum</i> and <i>Cylindrotheca</i> <i>fusiformis</i> to High Concentrations of Cu<sup>2+</sup> and Zn<sup>2+</sup>Aiyou Huang0Yujue Wang1Jiawen Duan2Shiyi Guo3Zhenyu Xie4State Key Laboratory of Marine Resource Utilization in the South China Sea, Hainan University, Haikou 570228, ChinaState Key Laboratory of Marine Resource Utilization in the South China Sea, Hainan University, Haikou 570228, ChinaState Key Laboratory of Marine Resource Utilization in the South China Sea, Hainan University, Haikou 570228, ChinaState Key Laboratory of Marine Resource Utilization in the South China Sea, Hainan University, Haikou 570228, ChinaState Key Laboratory of Marine Resource Utilization in the South China Sea, Hainan University, Haikou 570228, ChinaDiatoms can be used as biosensors to assess aquatic environment quality, because they are widely distributed in almost all aquatic environments and show varied sensitivities toward heavy metal ions. The marine planktonic diatoms <i>Phaeodactylum tricornutum</i> (<i>P. tricornutum</i>) and <i>Cylindrotheca fusiformis</i> (<i>C. fusiformis</i>) are typical representatives of planktonic diatoms and benthic diatoms, respectively. <i>C. fusiformis</i> is very sensitive to changes in the concentration of heavy metal ions, and can be used as an indicator of the quality of the sedimental environment, while <i>P. tricornutum</i> can tolerate higher concentrations of heavy metal ions. To explore the potential difference in responses to heavy metal ions between planktonic and benthic diatoms, we compared the transcriptome of <i>P. tricornutum</i> and <i>C. fusiformis</i> under Cu<sup>2+</sup> and Zn<sup>2+</sup> treatment. The results indicated that <i>P. tricornutum</i> has several genes involved in ion transmembrane transport and ion homeostasis, which are significantly downregulated under Cu<sup>2+</sup> and Zn<sup>2+</sup> treatment. However, this enrichment of ion transmembrane transport- and ion homeostasis-related genes was not observed in <i>C. fusiformis</i> under Cu<sup>2+</sup> and Zn<sup>2+</sup> treatment. Additionally, genes related to heavy metal ion stress response such as peroxiredoxin, peroxidase, catalase, glutathione metabolism, phytochelatin, oxidative stress and disulfide reductase, were upregulated in <i>P. tricornutum</i> under Cu<sup>2+</sup> and Zn<sup>2+</sup> treatment, whereas most of them were downregulated in <i>C. fusiformis</i> under Cu<sup>2+</sup> and Zn<sup>2+</sup> treatment. This difference in gene expression may be responsible for the difference in sensitivity to heavy metals between <i>P. tricornutum</i> and <i>C. fusiformis</i>.https://www.mdpi.com/2073-4441/14/20/3305diatom<i>Phaeodactylum tricornutum</i><i>Cylindrotheca fusiformis</i>heavy metalsbiological indicator |
| spellingShingle | Aiyou Huang Yujue Wang Jiawen Duan Shiyi Guo Zhenyu Xie Differential Response of <i>Phaeodactylum</i> <i>tricornutum</i> and <i>Cylindrotheca</i> <i>fusiformis</i> to High Concentrations of Cu<sup>2+</sup> and Zn<sup>2+</sup> Water diatom <i>Phaeodactylum tricornutum</i> <i>Cylindrotheca fusiformis</i> heavy metals biological indicator |
| title | Differential Response of <i>Phaeodactylum</i> <i>tricornutum</i> and <i>Cylindrotheca</i> <i>fusiformis</i> to High Concentrations of Cu<sup>2+</sup> and Zn<sup>2+</sup> |
| title_full | Differential Response of <i>Phaeodactylum</i> <i>tricornutum</i> and <i>Cylindrotheca</i> <i>fusiformis</i> to High Concentrations of Cu<sup>2+</sup> and Zn<sup>2+</sup> |
| title_fullStr | Differential Response of <i>Phaeodactylum</i> <i>tricornutum</i> and <i>Cylindrotheca</i> <i>fusiformis</i> to High Concentrations of Cu<sup>2+</sup> and Zn<sup>2+</sup> |
| title_full_unstemmed | Differential Response of <i>Phaeodactylum</i> <i>tricornutum</i> and <i>Cylindrotheca</i> <i>fusiformis</i> to High Concentrations of Cu<sup>2+</sup> and Zn<sup>2+</sup> |
| title_short | Differential Response of <i>Phaeodactylum</i> <i>tricornutum</i> and <i>Cylindrotheca</i> <i>fusiformis</i> to High Concentrations of Cu<sup>2+</sup> and Zn<sup>2+</sup> |
| title_sort | differential response of i phaeodactylum i i tricornutum i and i cylindrotheca i i fusiformis i to high concentrations of cu sup 2 sup and zn sup 2 sup |
| topic | diatom <i>Phaeodactylum tricornutum</i> <i>Cylindrotheca fusiformis</i> heavy metals biological indicator |
| url | https://www.mdpi.com/2073-4441/14/20/3305 |
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