lncRNA-Gm5532 regulates osteoclast differentiation through the miR-125a-3p/TRAF6 axis
Long noncoding RNAs (lncRNAs) are important regulators of bone metabolism. In this study, lncRNA microarray analysis was used to identify differentially expressed lncRNAs in differentiated osteoclasts. lncRNA-Gm5532 is highly expressed during osteoclast differentiation. lncRNA-Gm5532 knockdown impai...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
China Science Publishing & Media Ltd.
2023-12-01
|
| Series: | Acta Biochimica et Biophysica Sinica |
| Subjects: | |
| Online Access: | https://www.sciengine.com/doi/10.3724/abbs.2023245 |
| _version_ | 1797347181188874240 |
|---|---|
| author | Zhang Jian Zhang Lingyan Yao Gang Zhao Hai Qiao Penghai Wu Shuguang |
| author_facet | Zhang Jian Zhang Lingyan Yao Gang Zhao Hai Qiao Penghai Wu Shuguang |
| author_sort | Zhang Jian |
| collection | DOAJ |
| description | Long noncoding RNAs (lncRNAs) are important regulators of bone metabolism. In this study, lncRNA microarray analysis was used to identify differentially expressed lncRNAs in differentiated osteoclasts. lncRNA-Gm5532 is highly expressed during osteoclast differentiation. lncRNA-Gm5532 knockdown impairs osteoclast formation and bone resorption. Mechanistic experiments show that lncRNA-Gm5532 functions as a competing endogenous RNA (ceRNA) and acts as a sponge for miR-125a-3p, which promotes TNF receptor-associated factor 6 (TRAF6) expression. miR-125a-3p mimics suppress osteoclast differentiation and TAK1/NF-κB/MAPK signaling. The miR-125a-3p inhibitor reverses the negative effects of siGm5532 on osteoclast differentiation. In summary, our study reveals that lncRNA-Gm5532 functions as an activator in osteoclast differentiation by targeting the miR-125a-3p/TRAF6 axis, making it a novel biomarker and potential therapeutic target for osteoporosis. |
| first_indexed | 2024-03-08T11:43:58Z |
| format | Article |
| id | doaj.art-e9735af5121148c69650993c165153b9 |
| institution | Directory Open Access Journal |
| issn | 1672-9145 |
| language | English |
| last_indexed | 2024-03-08T11:43:58Z |
| publishDate | 2023-12-01 |
| publisher | China Science Publishing & Media Ltd. |
| record_format | Article |
| series | Acta Biochimica et Biophysica Sinica |
| spelling | doaj.art-e9735af5121148c69650993c165153b92024-01-25T01:50:58ZengChina Science Publishing & Media Ltd.Acta Biochimica et Biophysica Sinica1672-91452023-12-0156546110.3724/abbs.202324520d259cclncRNA-Gm5532 regulates osteoclast differentiation through the miR-125a-3p/TRAF6 axisZhang Jian0Zhang Lingyan1Yao Gang2Zhao Hai3Qiao Penghai4Wu Shuguang5[][][][][][]Long noncoding RNAs (lncRNAs) are important regulators of bone metabolism. In this study, lncRNA microarray analysis was used to identify differentially expressed lncRNAs in differentiated osteoclasts. lncRNA-Gm5532 is highly expressed during osteoclast differentiation. lncRNA-Gm5532 knockdown impairs osteoclast formation and bone resorption. Mechanistic experiments show that lncRNA-Gm5532 functions as a competing endogenous RNA (ceRNA) and acts as a sponge for miR-125a-3p, which promotes TNF receptor-associated factor 6 (TRAF6) expression. miR-125a-3p mimics suppress osteoclast differentiation and TAK1/NF-κB/MAPK signaling. The miR-125a-3p inhibitor reverses the negative effects of siGm5532 on osteoclast differentiation. In summary, our study reveals that lncRNA-Gm5532 functions as an activator in osteoclast differentiation by targeting the miR-125a-3p/TRAF6 axis, making it a novel biomarker and potential therapeutic target for osteoporosis.https://www.sciengine.com/doi/10.3724/abbs.2023245lncRNA-Gm5532osteoclast differentiationmiR-125a-3pTNF receptor-associated factor 6 (TRAF6) |
| spellingShingle | Zhang Jian Zhang Lingyan Yao Gang Zhao Hai Qiao Penghai Wu Shuguang lncRNA-Gm5532 regulates osteoclast differentiation through the miR-125a-3p/TRAF6 axis Acta Biochimica et Biophysica Sinica lncRNA-Gm5532 osteoclast differentiation miR-125a-3p TNF receptor-associated factor 6 (TRAF6) |
| title | lncRNA-Gm5532 regulates osteoclast differentiation through the miR-125a-3p/TRAF6 axis |
| title_full | lncRNA-Gm5532 regulates osteoclast differentiation through the miR-125a-3p/TRAF6 axis |
| title_fullStr | lncRNA-Gm5532 regulates osteoclast differentiation through the miR-125a-3p/TRAF6 axis |
| title_full_unstemmed | lncRNA-Gm5532 regulates osteoclast differentiation through the miR-125a-3p/TRAF6 axis |
| title_short | lncRNA-Gm5532 regulates osteoclast differentiation through the miR-125a-3p/TRAF6 axis |
| title_sort | lncrna gm5532 regulates osteoclast differentiation through the mir 125a 3p traf6 axis |
| topic | lncRNA-Gm5532 osteoclast differentiation miR-125a-3p TNF receptor-associated factor 6 (TRAF6) |
| url | https://www.sciengine.com/doi/10.3724/abbs.2023245 |
| work_keys_str_mv | AT zhangjian lncrnagm5532regulatesosteoclastdifferentiationthroughthemir125a3ptraf6axis AT zhanglingyan lncrnagm5532regulatesosteoclastdifferentiationthroughthemir125a3ptraf6axis AT yaogang lncrnagm5532regulatesosteoclastdifferentiationthroughthemir125a3ptraf6axis AT zhaohai lncrnagm5532regulatesosteoclastdifferentiationthroughthemir125a3ptraf6axis AT qiaopenghai lncrnagm5532regulatesosteoclastdifferentiationthroughthemir125a3ptraf6axis AT wushuguang lncrnagm5532regulatesosteoclastdifferentiationthroughthemir125a3ptraf6axis |