Light modulates activity and expression of glutamine synthetase isoforms in maize seedling roots
In maize roots, continuous illumination inhibits chloroplastic glutamine synthetase (GS2) activity, which decreased in light from 72.8% in 4-day-old to 26% in 10-day-old plants. In dark-adapted plants transferred to light for 6 days, GS2 activity declined from 100% to 41%, but in light-adapted plant...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2008-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2008/0354-46640804649S.pdf |
| _version_ | 1818759533721288704 |
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| author | Simonović Ana D. Anderson M.D. |
| author_facet | Simonović Ana D. Anderson M.D. |
| author_sort | Simonović Ana D. |
| collection | DOAJ |
| description | In maize roots, continuous illumination inhibits chloroplastic glutamine synthetase (GS2) activity, which decreased in light from 72.8% in 4-day-old to 26% in 10-day-old plants. In dark-adapted plants transferred to light for 6 days, GS2 activity declined from 100% to 41%, but in light-adapted plants transferred to darkness, it increased to the level of the dark control. Changes of cytosolic (GS1) activity were minor, with a similar trend. Quantitative RT-PCR revealed that light/dark treatments moderately affected only transcription of GS1 isoforms, with the exception of GS1-2, which was dramatically induced by darkness and repressed by light. |
| first_indexed | 2024-12-18T06:44:15Z |
| format | Article |
| id | doaj.art-e97507cb0b1a4b628cc1f507e03a18c7 |
| institution | Directory Open Access Journal |
| issn | 0354-4664 |
| language | English |
| last_indexed | 2024-12-18T06:44:15Z |
| publishDate | 2008-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-e97507cb0b1a4b628cc1f507e03a18c72022-12-21T21:17:32ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46642008-01-0160464966010.2298/ABS0804649SLight modulates activity and expression of glutamine synthetase isoforms in maize seedling rootsSimonović Ana D.Anderson M.D.In maize roots, continuous illumination inhibits chloroplastic glutamine synthetase (GS2) activity, which decreased in light from 72.8% in 4-day-old to 26% in 10-day-old plants. In dark-adapted plants transferred to light for 6 days, GS2 activity declined from 100% to 41%, but in light-adapted plants transferred to darkness, it increased to the level of the dark control. Changes of cytosolic (GS1) activity were minor, with a similar trend. Quantitative RT-PCR revealed that light/dark treatments moderately affected only transcription of GS1 isoforms, with the exception of GS1-2, which was dramatically induced by darkness and repressed by light.http://www.doiserbia.nb.rs/img/doi/0354-4664/2008/0354-46640804649S.pdfExpressionglutamine synthetaseGS1GS2GS1-2maizelightreal-time PCRZea mays |
| spellingShingle | Simonović Ana D. Anderson M.D. Light modulates activity and expression of glutamine synthetase isoforms in maize seedling roots Archives of Biological Sciences Expression glutamine synthetase GS1 GS2 GS1-2 maize light real-time PCR Zea mays |
| title | Light modulates activity and expression of glutamine synthetase isoforms in maize seedling roots |
| title_full | Light modulates activity and expression of glutamine synthetase isoforms in maize seedling roots |
| title_fullStr | Light modulates activity and expression of glutamine synthetase isoforms in maize seedling roots |
| title_full_unstemmed | Light modulates activity and expression of glutamine synthetase isoforms in maize seedling roots |
| title_short | Light modulates activity and expression of glutamine synthetase isoforms in maize seedling roots |
| title_sort | light modulates activity and expression of glutamine synthetase isoforms in maize seedling roots |
| topic | Expression glutamine synthetase GS1 GS2 GS1-2 maize light real-time PCR Zea mays |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2008/0354-46640804649S.pdf |
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