MALDI-TOF Mass Spectrometry Online Identification of <i>Trichophyton indotineae</i> Using the MSI-2 Application
<i>Trichophyton indotineae</i> is an emerging pathogen which recently spread from India to Europe and that is more prone than other species of the <i>Trichophyton mentagrophytes</i> complex to show resistance to terbinafine, resulting in the necessity of rapid identification....
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/10/1103 |
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| author | Anne-Cécile Normand Alicia Moreno-Sabater Arnaud Jabet Samia Hamane Geneviève Cremer Françoise Foulet Marion Blaize Sarah Dellière Christine Bonnal Sébastien Imbert Sophie Brun Ann Packeu Stéphane Bretagne Renaud Piarroux |
| author_facet | Anne-Cécile Normand Alicia Moreno-Sabater Arnaud Jabet Samia Hamane Geneviève Cremer Françoise Foulet Marion Blaize Sarah Dellière Christine Bonnal Sébastien Imbert Sophie Brun Ann Packeu Stéphane Bretagne Renaud Piarroux |
| author_sort | Anne-Cécile Normand |
| collection | DOAJ |
| description | <i>Trichophyton indotineae</i> is an emerging pathogen which recently spread from India to Europe and that is more prone than other species of the <i>Trichophyton mentagrophytes</i> complex to show resistance to terbinafine, resulting in the necessity of rapid identification. Here, we improved the online MSI-2 MALDI-TOF identification tool in order to identify <i>T. indotineae</i>. By multiplying the culture conditions (2 culture media and 6 stages of growth) prior to protein extractions for both test isolates and reference strains, we added 142 references corresponding to 12 strains inside the <i>T. mentagrophytes</i> complex in the online MSI-2 database, of which 3 are <i>T. indotineae</i> strains. The resulting database was tested with 1566 spectra of 67 isolates from the <i>T. mentagrophytes</i> complex, including 16 <i>T. indotineae</i> isolates. Using the newly improved MSI-2 database, we increased the identification rate of <i>T. indotineae</i> from 5% to 96%, with a sensitivity of 99.6%. We also identified specific peaks (6834/6845 daltons and 10,634/10,680 daltons) allowing for the distinction of <i>T. indotineae</i> from the other species of the complex. Our improved version of the MSI-2 application allows for the identification of <i>T. indotineae</i>. This will improve the epidemiological knowledge of the spread of this species throughout the world and will help to improve patient care. |
| first_indexed | 2024-03-09T20:00:28Z |
| format | Article |
| id | doaj.art-e97557139cc741908915507697e3ee90 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T20:00:28Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-e97557139cc741908915507697e3ee902023-11-24T00:47:59ZengMDPI AGJournal of Fungi2309-608X2022-10-01810110310.3390/jof8101103MALDI-TOF Mass Spectrometry Online Identification of <i>Trichophyton indotineae</i> Using the MSI-2 ApplicationAnne-Cécile Normand0Alicia Moreno-Sabater1Arnaud Jabet2Samia Hamane3Geneviève Cremer4Françoise Foulet5Marion Blaize6Sarah Dellière7Christine Bonnal8Sébastien Imbert9Sophie Brun10Ann Packeu11Stéphane Bretagne12Renaud Piarroux13Service de Parasitologie-Mycologie, Hôpital La Pitié-Salpêtrière, AP-HP, 75013 Paris, FranceService de Parasitologie-Mycologie, Hôpital Saint-Antoine, AP-HP, 75012 Paris, FranceService de Parasitologie-Mycologie, Hôpital La Pitié-Salpêtrière, AP-HP, 75013 Paris, FranceHôpital Saint Louis Laboratoire de Parasitologie-Mycologie, Assistance Publique des Hôpitaux de Paris, 75010 Paris, FranceLaboratoire Bioclinic, 75009 Paris, FranceService de Parasitologie-Mycologie, Hôpitaux Universitaires Henri Mondor, AP-HP, 94000 Créteil, FranceService de Parasitologie-Mycologie, Hôpital La Pitié-Salpêtrière, AP-HP, 75013 Paris, FranceHôpital Saint Louis Laboratoire de Parasitologie-Mycologie, Assistance Publique des Hôpitaux de Paris, 75010 Paris, FranceService de Parasitologie-Mycologie, Hôpital Bichat-Claude Bernard, AP-HP, 75018 Paris, FranceUniv Bordeaux, Centre de Recherche Cardio-Thoracique de Bordeaux, Inserm UMR 1045, 33600 Bordeaux, FranceService de Parasitologie-Mycologie, Hôpital Avicenne, AP-HP, 93009 Bobigny, FranceSciensano, BCCM/IHEM Collection, Mycology and Aerobiology Unit, 1000 Brussels, BelgiumHôpital Saint Louis Laboratoire de Parasitologie-Mycologie, Assistance Publique des Hôpitaux de Paris, 75010 Paris, FranceService de Parasitologie-Mycologie, Hôpital La Pitié-Salpêtrière, AP-HP, 75013 Paris, France<i>Trichophyton indotineae</i> is an emerging pathogen which recently spread from India to Europe and that is more prone than other species of the <i>Trichophyton mentagrophytes</i> complex to show resistance to terbinafine, resulting in the necessity of rapid identification. Here, we improved the online MSI-2 MALDI-TOF identification tool in order to identify <i>T. indotineae</i>. By multiplying the culture conditions (2 culture media and 6 stages of growth) prior to protein extractions for both test isolates and reference strains, we added 142 references corresponding to 12 strains inside the <i>T. mentagrophytes</i> complex in the online MSI-2 database, of which 3 are <i>T. indotineae</i> strains. The resulting database was tested with 1566 spectra of 67 isolates from the <i>T. mentagrophytes</i> complex, including 16 <i>T. indotineae</i> isolates. Using the newly improved MSI-2 database, we increased the identification rate of <i>T. indotineae</i> from 5% to 96%, with a sensitivity of 99.6%. We also identified specific peaks (6834/6845 daltons and 10,634/10,680 daltons) allowing for the distinction of <i>T. indotineae</i> from the other species of the complex. Our improved version of the MSI-2 application allows for the identification of <i>T. indotineae</i>. This will improve the epidemiological knowledge of the spread of this species throughout the world and will help to improve patient care.https://www.mdpi.com/2309-608X/8/10/1103MALDI-TOF mass spectrometry<i>Trichophyton indotineae</i><i>Trichophyton mentagrophytes</i> species complexMSI-2fungidermatophyte |
| spellingShingle | Anne-Cécile Normand Alicia Moreno-Sabater Arnaud Jabet Samia Hamane Geneviève Cremer Françoise Foulet Marion Blaize Sarah Dellière Christine Bonnal Sébastien Imbert Sophie Brun Ann Packeu Stéphane Bretagne Renaud Piarroux MALDI-TOF Mass Spectrometry Online Identification of <i>Trichophyton indotineae</i> Using the MSI-2 Application Journal of Fungi MALDI-TOF mass spectrometry <i>Trichophyton indotineae</i> <i>Trichophyton mentagrophytes</i> species complex MSI-2 fungi dermatophyte |
| title | MALDI-TOF Mass Spectrometry Online Identification of <i>Trichophyton indotineae</i> Using the MSI-2 Application |
| title_full | MALDI-TOF Mass Spectrometry Online Identification of <i>Trichophyton indotineae</i> Using the MSI-2 Application |
| title_fullStr | MALDI-TOF Mass Spectrometry Online Identification of <i>Trichophyton indotineae</i> Using the MSI-2 Application |
| title_full_unstemmed | MALDI-TOF Mass Spectrometry Online Identification of <i>Trichophyton indotineae</i> Using the MSI-2 Application |
| title_short | MALDI-TOF Mass Spectrometry Online Identification of <i>Trichophyton indotineae</i> Using the MSI-2 Application |
| title_sort | maldi tof mass spectrometry online identification of i trichophyton indotineae i using the msi 2 application |
| topic | MALDI-TOF mass spectrometry <i>Trichophyton indotineae</i> <i>Trichophyton mentagrophytes</i> species complex MSI-2 fungi dermatophyte |
| url | https://www.mdpi.com/2309-608X/8/10/1103 |
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