ENST00000534735 inhibits proliferation and migration, promotes apoptosis and pyroptosis of endometrial cancer via OSBPL3 through APMK/SIRT1/NF-κB pathway
Background: The complete understanding of the biological roles of long non-coding RNAs (lncRNAs) in cancer remains elusive. The findings of this study indicate that the newly discovered lncRNA ENST00000534735 exhibited a decreased expression in both endometrial cancer (EC) tissues and cell lines. Me...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-02-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024013124 |
| _version_ | 1797267849551544320 |
|---|---|
| author | Shuzhi Shan Xiao Wang Lijie Qian Chunxiao Wang Sufen Zhao |
| author_facet | Shuzhi Shan Xiao Wang Lijie Qian Chunxiao Wang Sufen Zhao |
| author_sort | Shuzhi Shan |
| collection | DOAJ |
| description | Background: The complete understanding of the biological roles of long non-coding RNAs (lncRNAs) in cancer remains elusive. The findings of this study indicate that the newly discovered lncRNA ENST00000534735 exhibited a decreased expression in both endometrial cancer (EC) tissues and cell lines. Methods: The expression of ENST00000534735 in EC tissues was detected using RNA-sequencing analysis. The effects of ENST00000534735 on cell proliferation, migration, apoptosis, and pyroptosis were determined via in vitro and in vivo experiments. The proteins that interact with ENST00000534735 were confirmed by RNA pull-down assay. Furthermore, an investigation was conducted on the impact of ENST00000534735 on the in vivo growth of EC through a tumorigenicity assay in nude mice. Results: We found that ENST00000534735 was significantly down-regulated in EC tissues compared to their adjacent non-cancerous tissues. The ectopic expression of ENST00000534735 drastically inhibited lung cancer cell proliferation and migration ability and facilitated apoptosis and pyroptosis. Knockdown of ENST00000534735 increased OSBPL3 expression, and the tumor-suppressing effects of ENST00000534735 overexpression were reversed by upregulation of OSBPL3 via the APMK/SIRT1/NF-κB pathway. The in vivo tumorigenic assays conducted on nude mice revealed that the excessive expression of ENST00000534735 impeded the growth of EC. Conclusions: All results elucidated the role and molecular mechanism of ENST00000534735 in the malignant development of EC. ENST00000534735, a new antioncogene in EC, may serve as a survival biomarker or therapeutic target for EC. |
| first_indexed | 2024-03-08T03:30:47Z |
| format | Article |
| id | doaj.art-e975c5bb97e043dc8c6acd7ff8a9765f |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-25T01:23:07Z |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-e975c5bb97e043dc8c6acd7ff8a9765f2024-03-09T09:25:18ZengElsevierHeliyon2405-84402024-02-01104e25281ENST00000534735 inhibits proliferation and migration, promotes apoptosis and pyroptosis of endometrial cancer via OSBPL3 through APMK/SIRT1/NF-κB pathwayShuzhi Shan0Xiao Wang1Lijie Qian2Chunxiao Wang3Sufen Zhao4Department of Gynecology and Obstetrics, The Second Hospital of Hebei Medical University, Shijiazhuang, ChinaDepartment of Gynecology and Obstetrics, The Second Hospital of Hebei Medical University, Shijiazhuang, ChinaHebei Women and Children's Health Center, Shijiazhuang, ChinaDepartment of Gynecology, Cangzhou People's Hospital, Cangzhou, ChinaDepartment of Gynecology and Obstetrics, The Second Hospital of Hebei Medical University, Shijiazhuang, China; Corresponding author. Department of Gynecology and Obstetrics, The Second Hospital of Hebei Medical University, 215 Heping West Road, Shijiazhuang, 050004, China.Background: The complete understanding of the biological roles of long non-coding RNAs (lncRNAs) in cancer remains elusive. The findings of this study indicate that the newly discovered lncRNA ENST00000534735 exhibited a decreased expression in both endometrial cancer (EC) tissues and cell lines. Methods: The expression of ENST00000534735 in EC tissues was detected using RNA-sequencing analysis. The effects of ENST00000534735 on cell proliferation, migration, apoptosis, and pyroptosis were determined via in vitro and in vivo experiments. The proteins that interact with ENST00000534735 were confirmed by RNA pull-down assay. Furthermore, an investigation was conducted on the impact of ENST00000534735 on the in vivo growth of EC through a tumorigenicity assay in nude mice. Results: We found that ENST00000534735 was significantly down-regulated in EC tissues compared to their adjacent non-cancerous tissues. The ectopic expression of ENST00000534735 drastically inhibited lung cancer cell proliferation and migration ability and facilitated apoptosis and pyroptosis. Knockdown of ENST00000534735 increased OSBPL3 expression, and the tumor-suppressing effects of ENST00000534735 overexpression were reversed by upregulation of OSBPL3 via the APMK/SIRT1/NF-κB pathway. The in vivo tumorigenic assays conducted on nude mice revealed that the excessive expression of ENST00000534735 impeded the growth of EC. Conclusions: All results elucidated the role and molecular mechanism of ENST00000534735 in the malignant development of EC. ENST00000534735, a new antioncogene in EC, may serve as a survival biomarker or therapeutic target for EC.http://www.sciencedirect.com/science/article/pii/S2405844024013124ENST00000534735Endometrial cancer (EC)PyroptosisApoptosisOSBPL3APMK/SIRT1/NF-κB |
| spellingShingle | Shuzhi Shan Xiao Wang Lijie Qian Chunxiao Wang Sufen Zhao ENST00000534735 inhibits proliferation and migration, promotes apoptosis and pyroptosis of endometrial cancer via OSBPL3 through APMK/SIRT1/NF-κB pathway Heliyon ENST00000534735 Endometrial cancer (EC) Pyroptosis Apoptosis OSBPL3 APMK/SIRT1/NF-κB |
| title | ENST00000534735 inhibits proliferation and migration, promotes apoptosis and pyroptosis of endometrial cancer via OSBPL3 through APMK/SIRT1/NF-κB pathway |
| title_full | ENST00000534735 inhibits proliferation and migration, promotes apoptosis and pyroptosis of endometrial cancer via OSBPL3 through APMK/SIRT1/NF-κB pathway |
| title_fullStr | ENST00000534735 inhibits proliferation and migration, promotes apoptosis and pyroptosis of endometrial cancer via OSBPL3 through APMK/SIRT1/NF-κB pathway |
| title_full_unstemmed | ENST00000534735 inhibits proliferation and migration, promotes apoptosis and pyroptosis of endometrial cancer via OSBPL3 through APMK/SIRT1/NF-κB pathway |
| title_short | ENST00000534735 inhibits proliferation and migration, promotes apoptosis and pyroptosis of endometrial cancer via OSBPL3 through APMK/SIRT1/NF-κB pathway |
| title_sort | enst00000534735 inhibits proliferation and migration promotes apoptosis and pyroptosis of endometrial cancer via osbpl3 through apmk sirt1 nf κb pathway |
| topic | ENST00000534735 Endometrial cancer (EC) Pyroptosis Apoptosis OSBPL3 APMK/SIRT1/NF-κB |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024013124 |
| work_keys_str_mv | AT shuzhishan enst00000534735inhibitsproliferationandmigrationpromotesapoptosisandpyroptosisofendometrialcancerviaosbpl3throughapmksirt1nfkbpathway AT xiaowang enst00000534735inhibitsproliferationandmigrationpromotesapoptosisandpyroptosisofendometrialcancerviaosbpl3throughapmksirt1nfkbpathway AT lijieqian enst00000534735inhibitsproliferationandmigrationpromotesapoptosisandpyroptosisofendometrialcancerviaosbpl3throughapmksirt1nfkbpathway AT chunxiaowang enst00000534735inhibitsproliferationandmigrationpromotesapoptosisandpyroptosisofendometrialcancerviaosbpl3throughapmksirt1nfkbpathway AT sufenzhao enst00000534735inhibitsproliferationandmigrationpromotesapoptosisandpyroptosisofendometrialcancerviaosbpl3throughapmksirt1nfkbpathway |