Streptomyces colonosanans sp. nov., A Novel Actinobacterium Isolated from Malaysia Mangrove Soil Exhibiting Antioxidative Activity and Cytotoxic Potential against Human Colon Cancer Cell Lines
Streptomyces colonosanans MUSC 93JT, a novel strain isolated from mangrove forest soil located at Sarawak, Malaysia. The bacterium was noted to be Gram-positive and to form light yellow aerial and vivid yellow substrate mycelium on ISP 2 agar. The polyphasic approach was used to determine the taxono...
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| Format: | Article |
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Frontiers Media S.A.
2017-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.00877/full |
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| author | Jodi Woan-Fei Law Hooi-Leng Ser Acharaporn Duangjai Acharaporn Duangjai Surasak Saokaew Surasak Saokaew Surasak Saokaew Sarah I. Bukhari Tahir M. Khan Tahir M. Khan Nurul-Syakima Ab Mutalib Kok-Gan Chan Bey-Hing Goh Bey-Hing Goh Learn-Han Lee Learn-Han Lee |
| author_facet | Jodi Woan-Fei Law Hooi-Leng Ser Acharaporn Duangjai Acharaporn Duangjai Surasak Saokaew Surasak Saokaew Surasak Saokaew Sarah I. Bukhari Tahir M. Khan Tahir M. Khan Nurul-Syakima Ab Mutalib Kok-Gan Chan Bey-Hing Goh Bey-Hing Goh Learn-Han Lee Learn-Han Lee |
| author_sort | Jodi Woan-Fei Law |
| collection | DOAJ |
| description | Streptomyces colonosanans MUSC 93JT, a novel strain isolated from mangrove forest soil located at Sarawak, Malaysia. The bacterium was noted to be Gram-positive and to form light yellow aerial and vivid yellow substrate mycelium on ISP 2 agar. The polyphasic approach was used to determine the taxonomy of strain MUSC 93JT and the strain showed a range of phylogenetic and chemotaxonomic properties consistent with those of the members of the genus Streptomyces. Phylogenetic and 16S rRNA gene sequence analysis indicated that closely related strains include Streptomyces malachitofuscus NBRC 13059T (99.2% sequence similarity), Streptomyces misionensis NBRC 13063T (99.1%), and Streptomyces phaeoluteichromatogenes NRRL 5799T (99.1%). The DNA–DNA relatedness values between MUSC 93JT and closely related type strains ranged from 14.4 ± 0.1 to 46.2 ± 0.4%. The comparison of BOX-PCR fingerprints indicated MUSC 93JT exhibits a unique DNA profile. The genome of MUSC 93JT consists of 7,015,076 bp. The DNA G + C content was determined to be 69.90 mol%. The extract of strain MUSC 93JT was demonstrated to exhibit potent antioxidant activity via ABTS, metal chelating, and SOD assays. This extract also exhibited anticancer activity against human colon cancer cell lines without significant cytotoxic effect against human normal colon cells. Furthermore, the chemical analysis of the extract further emphasizes the strain is producing chemo-preventive related metabolites. Based on this polyphasic study of MUSC 93JT, it is concluded that this strain represents a novel species, for which the name Streptomyces colonosanans sp. nov. is proposed. The type strain is MUSC 93JT (= DSM 102042T = MCCC 1K02298T). |
| first_indexed | 2024-12-23T20:16:00Z |
| format | Article |
| id | doaj.art-e97ad33a5c09451e8cba1f40f070e61c |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-23T20:16:00Z |
| publishDate | 2017-05-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-e97ad33a5c09451e8cba1f40f070e61c2022-12-21T17:32:40ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-05-01810.3389/fmicb.2017.00877260348Streptomyces colonosanans sp. nov., A Novel Actinobacterium Isolated from Malaysia Mangrove Soil Exhibiting Antioxidative Activity and Cytotoxic Potential against Human Colon Cancer Cell LinesJodi Woan-Fei Law0Hooi-Leng Ser1Acharaporn Duangjai2Acharaporn Duangjai3Surasak Saokaew4Surasak Saokaew5Surasak Saokaew6Sarah I. Bukhari7Tahir M. Khan8Tahir M. Khan9Nurul-Syakima Ab Mutalib10Kok-Gan Chan11Bey-Hing Goh12Bey-Hing Goh13Learn-Han Lee14Learn-Han Lee15Novel Bacteria and Drug Discovery Research Group, School of Pharmacy, Monash University MalaysiaBandar Sunway, MalaysiaNovel Bacteria and Drug Discovery Research Group, School of Pharmacy, Monash University MalaysiaBandar Sunway, MalaysiaDivision of Physiology, School of Medical Sciences, University of PhayaoPhayao, ThailandCenter of Health Outcomes Research and Therapeutic Safety, School of Pharmaceutical Sciences, University of PhayaoPhayao, ThailandNovel Bacteria and Drug Discovery Research Group, School of Pharmacy, Monash University MalaysiaBandar Sunway, MalaysiaCenter of Health Outcomes Research and Therapeutic Safety, School of Pharmaceutical Sciences, University of PhayaoPhayao, ThailandFaculty of Pharmaceutical Sciences, Pharmaceutical Outcomes Research Center, Naresuan UniversityPhitsanulok, ThailandDepartment of Pharmaceutics, College of Pharmacy, King Saud UniversityRiyadh, Saudi ArabiaNovel Bacteria and Drug Discovery Research Group, School of Pharmacy, Monash University MalaysiaBandar Sunway, MalaysiaDepartment of Pharmacy, Absyn University PeshawarPeshawar, PakistanUKM Medical Molecular Biology Institute, UKM Medical Centre, University Kebangsaan MalaysiaKuala Lumpur, MalaysiaDivision of Genetics and Molecular Biology, Faculty of Science, Institute of Biological Sciences, University of MalayaKuala Lumpur, MalaysiaNovel Bacteria and Drug Discovery Research Group, School of Pharmacy, Monash University MalaysiaBandar Sunway, MalaysiaCenter of Health Outcomes Research and Therapeutic Safety, School of Pharmaceutical Sciences, University of PhayaoPhayao, ThailandNovel Bacteria and Drug Discovery Research Group, School of Pharmacy, Monash University MalaysiaBandar Sunway, MalaysiaCenter of Health Outcomes Research and Therapeutic Safety, School of Pharmaceutical Sciences, University of PhayaoPhayao, ThailandStreptomyces colonosanans MUSC 93JT, a novel strain isolated from mangrove forest soil located at Sarawak, Malaysia. The bacterium was noted to be Gram-positive and to form light yellow aerial and vivid yellow substrate mycelium on ISP 2 agar. The polyphasic approach was used to determine the taxonomy of strain MUSC 93JT and the strain showed a range of phylogenetic and chemotaxonomic properties consistent with those of the members of the genus Streptomyces. Phylogenetic and 16S rRNA gene sequence analysis indicated that closely related strains include Streptomyces malachitofuscus NBRC 13059T (99.2% sequence similarity), Streptomyces misionensis NBRC 13063T (99.1%), and Streptomyces phaeoluteichromatogenes NRRL 5799T (99.1%). The DNA–DNA relatedness values between MUSC 93JT and closely related type strains ranged from 14.4 ± 0.1 to 46.2 ± 0.4%. The comparison of BOX-PCR fingerprints indicated MUSC 93JT exhibits a unique DNA profile. The genome of MUSC 93JT consists of 7,015,076 bp. The DNA G + C content was determined to be 69.90 mol%. The extract of strain MUSC 93JT was demonstrated to exhibit potent antioxidant activity via ABTS, metal chelating, and SOD assays. This extract also exhibited anticancer activity against human colon cancer cell lines without significant cytotoxic effect against human normal colon cells. Furthermore, the chemical analysis of the extract further emphasizes the strain is producing chemo-preventive related metabolites. Based on this polyphasic study of MUSC 93JT, it is concluded that this strain represents a novel species, for which the name Streptomyces colonosanans sp. nov. is proposed. The type strain is MUSC 93JT (= DSM 102042T = MCCC 1K02298T).http://journal.frontiersin.org/article/10.3389/fmicb.2017.00877/fullStreptomyces colonosanansactinobacteriamangroveantioxidantcancer |
| spellingShingle | Jodi Woan-Fei Law Hooi-Leng Ser Acharaporn Duangjai Acharaporn Duangjai Surasak Saokaew Surasak Saokaew Surasak Saokaew Sarah I. Bukhari Tahir M. Khan Tahir M. Khan Nurul-Syakima Ab Mutalib Kok-Gan Chan Bey-Hing Goh Bey-Hing Goh Learn-Han Lee Learn-Han Lee Streptomyces colonosanans sp. nov., A Novel Actinobacterium Isolated from Malaysia Mangrove Soil Exhibiting Antioxidative Activity and Cytotoxic Potential against Human Colon Cancer Cell Lines Frontiers in Microbiology Streptomyces colonosanans actinobacteria mangrove antioxidant cancer |
| title | Streptomyces colonosanans sp. nov., A Novel Actinobacterium Isolated from Malaysia Mangrove Soil Exhibiting Antioxidative Activity and Cytotoxic Potential against Human Colon Cancer Cell Lines |
| title_full | Streptomyces colonosanans sp. nov., A Novel Actinobacterium Isolated from Malaysia Mangrove Soil Exhibiting Antioxidative Activity and Cytotoxic Potential against Human Colon Cancer Cell Lines |
| title_fullStr | Streptomyces colonosanans sp. nov., A Novel Actinobacterium Isolated from Malaysia Mangrove Soil Exhibiting Antioxidative Activity and Cytotoxic Potential against Human Colon Cancer Cell Lines |
| title_full_unstemmed | Streptomyces colonosanans sp. nov., A Novel Actinobacterium Isolated from Malaysia Mangrove Soil Exhibiting Antioxidative Activity and Cytotoxic Potential against Human Colon Cancer Cell Lines |
| title_short | Streptomyces colonosanans sp. nov., A Novel Actinobacterium Isolated from Malaysia Mangrove Soil Exhibiting Antioxidative Activity and Cytotoxic Potential against Human Colon Cancer Cell Lines |
| title_sort | streptomyces colonosanans sp nov a novel actinobacterium isolated from malaysia mangrove soil exhibiting antioxidative activity and cytotoxic potential against human colon cancer cell lines |
| topic | Streptomyces colonosanans actinobacteria mangrove antioxidant cancer |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.00877/full |
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