Identification of phenolics from miracle berry (Synsepalum dulcificum) leaf extract and its antiangiogenesis and anticancer activities
Miracle berry is well-known for its ability to convert sour foods to sweet. In this study, the secondary metabolites of miracle berry leaves (MBL) were identified by UPLC-DAD-MS, and its antiangiogenesis and anticancer activities were evaluated by using a zebrafish model and the MCF-7 xenograft mous...
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| Format: | Article |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.970019/full |
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| author | Fei-Yue Ma Fei-Yue Ma Fei-Yue Ma Fei-Yue Ma Xiu-Mei Zhang Xiu-Mei Zhang Xiu-Mei Zhang Ya Li Ya Li Ya Li Ming Zhang Ming Zhang Ming Zhang Xing-Hao Tu Xing-Hao Tu Xing-Hao Tu Li-Qing Du Li-Qing Du Li-Qing Du |
| author_facet | Fei-Yue Ma Fei-Yue Ma Fei-Yue Ma Fei-Yue Ma Xiu-Mei Zhang Xiu-Mei Zhang Xiu-Mei Zhang Ya Li Ya Li Ya Li Ming Zhang Ming Zhang Ming Zhang Xing-Hao Tu Xing-Hao Tu Xing-Hao Tu Li-Qing Du Li-Qing Du Li-Qing Du |
| author_sort | Fei-Yue Ma |
| collection | DOAJ |
| description | Miracle berry is well-known for its ability to convert sour foods to sweet. In this study, the secondary metabolites of miracle berry leaves (MBL) were identified by UPLC-DAD-MS, and its antiangiogenesis and anticancer activities were evaluated by using a zebrafish model and the MCF-7 xenograft mouse model, respectively. The result showed that 18 phenolic compounds were identified in MBL extract, and dominated by the derivatives of quercetin and myricetin. The MBL extract showed low toxicity and high antiangiogenesis activity, it significantly inhibited the subintestinal vein vessels development in zebrafish at very low concentration. Furthermore, the MBL extract could promote the apoptosis of tumor cells and significantly inhibit the growth of MCF-7 xenograft tumor. In addition, the analysis of metabolites revealed that the MBL extract inhibited tumor growth by activating the metabolic pathways of unsaturated fatty acids and purines. Overall, this study suggests that MBL extract can be used as a natural anticancer adjuvant in the fields of functional foods. |
| first_indexed | 2024-04-12T06:26:30Z |
| format | Article |
| id | doaj.art-e97c2850f498477ba4d8605ed2374381 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-12T06:26:30Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-e97c2850f498477ba4d8605ed23743812022-12-22T03:44:08ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-08-01910.3389/fnut.2022.970019970019Identification of phenolics from miracle berry (Synsepalum dulcificum) leaf extract and its antiangiogenesis and anticancer activitiesFei-Yue Ma0Fei-Yue Ma1Fei-Yue Ma2Fei-Yue Ma3Xiu-Mei Zhang4Xiu-Mei Zhang5Xiu-Mei Zhang6Ya Li7Ya Li8Ya Li9Ming Zhang10Ming Zhang11Ming Zhang12Xing-Hao Tu13Xing-Hao Tu14Xing-Hao Tu15Li-Qing Du16Li-Qing Du17Li-Qing Du18South Subtropical Crop Research Institute, Chinese Academy of Tropical Agricultural Science (CATAS), Zhanjiang, ChinaKey Laboratory of Tropical Fruit Biology, Ministry of Agriculture, Zhanjiang, ChinaKey Laboratory of Hainan Province for Post-Harvest Physiology and Technology of Tropical Horticultural Products, Zhanjiang, ChinaBaicheng Academy of Agricultural Sciences, Baicheng, ChinaSouth Subtropical Crop Research Institute, Chinese Academy of Tropical Agricultural Science (CATAS), Zhanjiang, ChinaKey Laboratory of Tropical Fruit Biology, Ministry of Agriculture, Zhanjiang, ChinaKey Laboratory of Hainan Province for Post-Harvest Physiology and Technology of Tropical Horticultural Products, Zhanjiang, ChinaSouth Subtropical Crop Research Institute, Chinese Academy of Tropical Agricultural Science (CATAS), Zhanjiang, ChinaKey Laboratory of Tropical Fruit Biology, Ministry of Agriculture, Zhanjiang, ChinaKey Laboratory of Hainan Province for Post-Harvest Physiology and Technology of Tropical Horticultural Products, Zhanjiang, ChinaSouth Subtropical Crop Research Institute, Chinese Academy of Tropical Agricultural Science (CATAS), Zhanjiang, ChinaKey Laboratory of Tropical Fruit Biology, Ministry of Agriculture, Zhanjiang, ChinaKey Laboratory of Hainan Province for Post-Harvest Physiology and Technology of Tropical Horticultural Products, Zhanjiang, ChinaSouth Subtropical Crop Research Institute, Chinese Academy of Tropical Agricultural Science (CATAS), Zhanjiang, ChinaKey Laboratory of Tropical Fruit Biology, Ministry of Agriculture, Zhanjiang, ChinaKey Laboratory of Hainan Province for Post-Harvest Physiology and Technology of Tropical Horticultural Products, Zhanjiang, ChinaSouth Subtropical Crop Research Institute, Chinese Academy of Tropical Agricultural Science (CATAS), Zhanjiang, ChinaKey Laboratory of Tropical Fruit Biology, Ministry of Agriculture, Zhanjiang, ChinaKey Laboratory of Hainan Province for Post-Harvest Physiology and Technology of Tropical Horticultural Products, Zhanjiang, ChinaMiracle berry is well-known for its ability to convert sour foods to sweet. In this study, the secondary metabolites of miracle berry leaves (MBL) were identified by UPLC-DAD-MS, and its antiangiogenesis and anticancer activities were evaluated by using a zebrafish model and the MCF-7 xenograft mouse model, respectively. The result showed that 18 phenolic compounds were identified in MBL extract, and dominated by the derivatives of quercetin and myricetin. The MBL extract showed low toxicity and high antiangiogenesis activity, it significantly inhibited the subintestinal vein vessels development in zebrafish at very low concentration. Furthermore, the MBL extract could promote the apoptosis of tumor cells and significantly inhibit the growth of MCF-7 xenograft tumor. In addition, the analysis of metabolites revealed that the MBL extract inhibited tumor growth by activating the metabolic pathways of unsaturated fatty acids and purines. Overall, this study suggests that MBL extract can be used as a natural anticancer adjuvant in the fields of functional foods.https://www.frontiersin.org/articles/10.3389/fnut.2022.970019/fullmiracle berryphenolicszebrafishMCF-7 xenograftmetabolomics |
| spellingShingle | Fei-Yue Ma Fei-Yue Ma Fei-Yue Ma Fei-Yue Ma Xiu-Mei Zhang Xiu-Mei Zhang Xiu-Mei Zhang Ya Li Ya Li Ya Li Ming Zhang Ming Zhang Ming Zhang Xing-Hao Tu Xing-Hao Tu Xing-Hao Tu Li-Qing Du Li-Qing Du Li-Qing Du Identification of phenolics from miracle berry (Synsepalum dulcificum) leaf extract and its antiangiogenesis and anticancer activities Frontiers in Nutrition miracle berry phenolics zebrafish MCF-7 xenograft metabolomics |
| title | Identification of phenolics from miracle berry (Synsepalum dulcificum) leaf extract and its antiangiogenesis and anticancer activities |
| title_full | Identification of phenolics from miracle berry (Synsepalum dulcificum) leaf extract and its antiangiogenesis and anticancer activities |
| title_fullStr | Identification of phenolics from miracle berry (Synsepalum dulcificum) leaf extract and its antiangiogenesis and anticancer activities |
| title_full_unstemmed | Identification of phenolics from miracle berry (Synsepalum dulcificum) leaf extract and its antiangiogenesis and anticancer activities |
| title_short | Identification of phenolics from miracle berry (Synsepalum dulcificum) leaf extract and its antiangiogenesis and anticancer activities |
| title_sort | identification of phenolics from miracle berry synsepalum dulcificum leaf extract and its antiangiogenesis and anticancer activities |
| topic | miracle berry phenolics zebrafish MCF-7 xenograft metabolomics |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.970019/full |
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