"INHIBITION ASSAY STUDY OF PURIFIED GLUTATHIONE S-TRANSFERASE FROM FASCIOLA HEPATICA AND SHEEP LIVER TISSUE BY HEXACHLOROPHENE"
Glutathione S-transferases (GSTs) are widespread in Fasciola. hepatica parasite and sheep liver tissue. Study of GSTs inhibition assays in F. hepatica and sheep liver tissue are a priority of chemotherapeutic targets in parasitic liver diseases including human fascioliasis in Iran. In this research,...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2004-08-01
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| Series: | Acta Medica Iranica |
| Subjects: | |
| Online Access: | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/378.pdf&manuscript_id=378 |
| _version_ | 1818239334058295296 |
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| author | A. Farahnak PM. Brophy |
| author_facet | A. Farahnak PM. Brophy |
| author_sort | A. Farahnak PM. Brophy |
| collection | DOAJ |
| description | Glutathione S-transferases (GSTs) are widespread in Fasciola. hepatica parasite and sheep liver tissue. Study of GSTs inhibition assays in F. hepatica and sheep liver tissue are a priority of chemotherapeutic targets in parasitic liver diseases including human fascioliasis in Iran. In this research, the whole extract of F. hepatica and sheep liver tissues were purified and eluted for sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) pattern and GSTs inhibition assay. GSTs inhibition was detected by hexachlorophene as an inhibitor and 1-chloro-2,4-dinitrobenzene (CDNB) as secondary substrate. The purified GSTs from F. hepatica and liver tissue contained comparable components and showed a molecular weight of 26kDa. The inhibitor concentration of hexachlorophene, for the remaining 50% activity (IC50%) of GST enzymes from F. hepatica and liver were graphically calculated, and the results were 0.25 µM and 1 µM, respectively. GSTs of F. hepatica may be more sensitive than sheep liver tissue to hexachlorophene. |
| first_indexed | 2024-12-12T12:55:53Z |
| format | Article |
| id | doaj.art-e97e85096d7e40969564d51f89523d6d |
| institution | Directory Open Access Journal |
| issn | 0044-6025 |
| language | English |
| last_indexed | 2024-12-12T12:55:53Z |
| publishDate | 2004-08-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Acta Medica Iranica |
| spelling | doaj.art-e97e85096d7e40969564d51f89523d6d2022-12-22T00:23:53ZengTehran University of Medical SciencesActa Medica Iranica0044-60252004-08-01423168171"INHIBITION ASSAY STUDY OF PURIFIED GLUTATHIONE S-TRANSFERASE FROM FASCIOLA HEPATICA AND SHEEP LIVER TISSUE BY HEXACHLOROPHENE"A. Farahnak PM. BrophyGlutathione S-transferases (GSTs) are widespread in Fasciola. hepatica parasite and sheep liver tissue. Study of GSTs inhibition assays in F. hepatica and sheep liver tissue are a priority of chemotherapeutic targets in parasitic liver diseases including human fascioliasis in Iran. In this research, the whole extract of F. hepatica and sheep liver tissues were purified and eluted for sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) pattern and GSTs inhibition assay. GSTs inhibition was detected by hexachlorophene as an inhibitor and 1-chloro-2,4-dinitrobenzene (CDNB) as secondary substrate. The purified GSTs from F. hepatica and liver tissue contained comparable components and showed a molecular weight of 26kDa. The inhibitor concentration of hexachlorophene, for the remaining 50% activity (IC50%) of GST enzymes from F. hepatica and liver were graphically calculated, and the results were 0.25 µM and 1 µM, respectively. GSTs of F. hepatica may be more sensitive than sheep liver tissue to hexachlorophene.http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/378.pdf&manuscript_id=378Glutathione S-transferaseinhibition assay |
| spellingShingle | A. Farahnak PM. Brophy "INHIBITION ASSAY STUDY OF PURIFIED GLUTATHIONE S-TRANSFERASE FROM FASCIOLA HEPATICA AND SHEEP LIVER TISSUE BY HEXACHLOROPHENE" Acta Medica Iranica Glutathione S-transferase inhibition assay |
| title | "INHIBITION ASSAY STUDY OF PURIFIED GLUTATHIONE S-TRANSFERASE FROM FASCIOLA HEPATICA AND SHEEP LIVER TISSUE BY HEXACHLOROPHENE" |
| title_full | "INHIBITION ASSAY STUDY OF PURIFIED GLUTATHIONE S-TRANSFERASE FROM FASCIOLA HEPATICA AND SHEEP LIVER TISSUE BY HEXACHLOROPHENE" |
| title_fullStr | "INHIBITION ASSAY STUDY OF PURIFIED GLUTATHIONE S-TRANSFERASE FROM FASCIOLA HEPATICA AND SHEEP LIVER TISSUE BY HEXACHLOROPHENE" |
| title_full_unstemmed | "INHIBITION ASSAY STUDY OF PURIFIED GLUTATHIONE S-TRANSFERASE FROM FASCIOLA HEPATICA AND SHEEP LIVER TISSUE BY HEXACHLOROPHENE" |
| title_short | "INHIBITION ASSAY STUDY OF PURIFIED GLUTATHIONE S-TRANSFERASE FROM FASCIOLA HEPATICA AND SHEEP LIVER TISSUE BY HEXACHLOROPHENE" |
| title_sort | inhibition assay study of purified glutathione s transferase from fasciola hepatica and sheep liver tissue by hexachlorophene |
| topic | Glutathione S-transferase inhibition assay |
| url | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/378.pdf&manuscript_id=378 |
| work_keys_str_mv | AT afarahnakpmbrophy inhibitionassaystudyofpurifiedglutathionestransferasefromfasciolahepaticaandsheeplivertissuebyhexachlorophene |