Simultaneous spectrofluorometric analysis of tablets containing hydrochlorothiazide combined with timolol maleate or amiloride hydrochloride
Green and sensitive spectrofluorometric methods have been developed and validated for the determination of timolol maleate (TML)/hydrochlorothiazide (HCT) and amiloride hydrochloride (AMH)/hydrochlorothiazide in tablets. The proposed spectrofluorometric procedures were found to be linear in the rang...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sciendo
2020-09-01
|
| Series: | Acta Pharmaceutica |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/acph-2020-0027 |
| _version_ | 1818607649294385152 |
|---|---|
| author | Wedian Fadel Lataifeh Anas Mohammed Makaseb S. |
| author_facet | Wedian Fadel Lataifeh Anas Mohammed Makaseb S. |
| author_sort | Wedian Fadel |
| collection | DOAJ |
| description | Green and sensitive spectrofluorometric methods have been developed and validated for the determination of timolol maleate (TML)/hydrochlorothiazide (HCT) and amiloride hydrochloride (AMH)/hydrochlorothiazide in tablets. The proposed spectrofluorometric procedures were found to be linear in the range of 4–12, 5–35 and 0.025–0.2 mg L−1 for HCT, TML and AMH, resp. The excitation and emission wavelengths for HCT, TML and AMH at room temperature were 270 and 375, 295 and 435, 330 and 415 nm, resp. The methods were validated with respect to ICH guidelines. The AMH showed higher sensitivity with lower values of LOD and LOQ values compared to HCT and TML. The proposed methods were applied to two pharmaceutical formulations; the method for HCT and AMH has proven as reliable assaying method, whereas the method for TML, when combined with HCT, is applicable to screening semi-quantitative analyses. |
| first_indexed | 2024-12-16T14:30:06Z |
| format | Article |
| id | doaj.art-e97fcc34fcfb40ccbb3c93f307393001 |
| institution | Directory Open Access Journal |
| issn | 1846-9558 |
| language | English |
| last_indexed | 2024-12-16T14:30:06Z |
| publishDate | 2020-09-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj.art-e97fcc34fcfb40ccbb3c93f3073930012022-12-21T22:28:16ZengSciendoActa Pharmaceutica1846-95582020-09-0170337338510.2478/acph-2020-0027acph-2020-0027Simultaneous spectrofluorometric analysis of tablets containing hydrochlorothiazide combined with timolol maleate or amiloride hydrochlorideWedian Fadel0Lataifeh Anas1Mohammed Makaseb S.2Department of Chemistry Faculty of Science, Yarmouk University P.O. Box 560, Irbid 22163, JordanDepartment of Chemistry Faculty of Science, Tafila Technical University, Tafila 66110, JordanDepartment of Chemistry Faculty of Science, Yarmouk University P.O. Box 560, Irbid 22163, JordanGreen and sensitive spectrofluorometric methods have been developed and validated for the determination of timolol maleate (TML)/hydrochlorothiazide (HCT) and amiloride hydrochloride (AMH)/hydrochlorothiazide in tablets. The proposed spectrofluorometric procedures were found to be linear in the range of 4–12, 5–35 and 0.025–0.2 mg L−1 for HCT, TML and AMH, resp. The excitation and emission wavelengths for HCT, TML and AMH at room temperature were 270 and 375, 295 and 435, 330 and 415 nm, resp. The methods were validated with respect to ICH guidelines. The AMH showed higher sensitivity with lower values of LOD and LOQ values compared to HCT and TML. The proposed methods were applied to two pharmaceutical formulations; the method for HCT and AMH has proven as reliable assaying method, whereas the method for TML, when combined with HCT, is applicable to screening semi-quantitative analyses.https://doi.org/10.2478/acph-2020-0027hydrochlorothiazideamiloride hydrochloridetimolol maleatefixed-dose combinationsspectrofluorometryassayingscreening |
| spellingShingle | Wedian Fadel Lataifeh Anas Mohammed Makaseb S. Simultaneous spectrofluorometric analysis of tablets containing hydrochlorothiazide combined with timolol maleate or amiloride hydrochloride Acta Pharmaceutica hydrochlorothiazide amiloride hydrochloride timolol maleate fixed-dose combinations spectrofluorometry assaying screening |
| title | Simultaneous spectrofluorometric analysis of tablets containing hydrochlorothiazide combined with timolol maleate or amiloride hydrochloride |
| title_full | Simultaneous spectrofluorometric analysis of tablets containing hydrochlorothiazide combined with timolol maleate or amiloride hydrochloride |
| title_fullStr | Simultaneous spectrofluorometric analysis of tablets containing hydrochlorothiazide combined with timolol maleate or amiloride hydrochloride |
| title_full_unstemmed | Simultaneous spectrofluorometric analysis of tablets containing hydrochlorothiazide combined with timolol maleate or amiloride hydrochloride |
| title_short | Simultaneous spectrofluorometric analysis of tablets containing hydrochlorothiazide combined with timolol maleate or amiloride hydrochloride |
| title_sort | simultaneous spectrofluorometric analysis of tablets containing hydrochlorothiazide combined with timolol maleate or amiloride hydrochloride |
| topic | hydrochlorothiazide amiloride hydrochloride timolol maleate fixed-dose combinations spectrofluorometry assaying screening |
| url | https://doi.org/10.2478/acph-2020-0027 |
| work_keys_str_mv | AT wedianfadel simultaneousspectrofluorometricanalysisoftabletscontaininghydrochlorothiazidecombinedwithtimololmaleateoramiloridehydrochloride AT lataifehanas simultaneousspectrofluorometricanalysisoftabletscontaininghydrochlorothiazidecombinedwithtimololmaleateoramiloridehydrochloride AT mohammedmakasebs simultaneousspectrofluorometricanalysisoftabletscontaininghydrochlorothiazidecombinedwithtimololmaleateoramiloridehydrochloride |