Macroporous Cell-Laden Gelatin/Hyaluronic Acid/Chondroitin Sulfate Cryogels for Engineered Tissue Constructs
Cryogels are a unique macroporous material for tissue engineering. In this work, we study the effect of hyaluronic acid on the physicochemical properties of cryogel as well as on the proliferation of a 3D model of mesenchymal stem cells. The functional groups of the synthesized cryogels were identif...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-09-01
|
| Series: | Gels |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2310-2861/8/9/590 |
| _version_ | 1827660820963655680 |
|---|---|
| author | Gulshakhar Kudaibergen Madina Zhunussova Ellina A. Mun Yerlan Ramankulov Vyacheslav Ogay |
| author_facet | Gulshakhar Kudaibergen Madina Zhunussova Ellina A. Mun Yerlan Ramankulov Vyacheslav Ogay |
| author_sort | Gulshakhar Kudaibergen |
| collection | DOAJ |
| description | Cryogels are a unique macroporous material for tissue engineering. In this work, we study the effect of hyaluronic acid on the physicochemical properties of cryogel as well as on the proliferation of a 3D model of mesenchymal stem cells. The functional groups of the synthesized cryogels were identified using Fourier transform infrared spectroscopy. With an increase in the content of hyaluronic acid in the composition of the cryogel, an increase in porosity, gel content and swelling behavior was observed. As the hyaluronic acid content increased, the average pore size increased and more open pores were formed. Degradation studies have shown that all cryogels were resistant to PBS solution for 8 weeks. Cytotoxicity assays demonstrated no toxic effect on viability of rat adipose-derived mesenchymal stem cells (ADMSCs) cultured on cryogels. ADMSC spheroids were proliferated on scaffolds and showed the ability of the cryogels to orient cell differentiation into chondrogenic lineage even in the absence of inductive agents. Thus, our results demonstrate an effective resemblance to extracellular matrix structures specific to cartilage-like microenvironments by cryogels and their further perspective application as potential biomaterials. |
| first_indexed | 2024-03-09T23:57:55Z |
| format | Article |
| id | doaj.art-e981017f25d047a49979732aa1f031f1 |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-09T23:57:55Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj.art-e981017f25d047a49979732aa1f031f12023-11-23T16:22:37ZengMDPI AGGels2310-28612022-09-018959010.3390/gels8090590Macroporous Cell-Laden Gelatin/Hyaluronic Acid/Chondroitin Sulfate Cryogels for Engineered Tissue ConstructsGulshakhar Kudaibergen0Madina Zhunussova1Ellina A. Mun2Yerlan Ramankulov3Vyacheslav Ogay4Stem Cell Laboratory, National Center for Biotechnology, Nur-Sultan 010000, KazakhstanStem Cell Laboratory, National Center for Biotechnology, Nur-Sultan 010000, KazakhstanSchool of Science and Humanities, Nazarbayev University, Nur-Sultan 010000, KazakhstanStem Cell Laboratory, National Center for Biotechnology, Nur-Sultan 010000, KazakhstanStem Cell Laboratory, National Center for Biotechnology, Nur-Sultan 010000, KazakhstanCryogels are a unique macroporous material for tissue engineering. In this work, we study the effect of hyaluronic acid on the physicochemical properties of cryogel as well as on the proliferation of a 3D model of mesenchymal stem cells. The functional groups of the synthesized cryogels were identified using Fourier transform infrared spectroscopy. With an increase in the content of hyaluronic acid in the composition of the cryogel, an increase in porosity, gel content and swelling behavior was observed. As the hyaluronic acid content increased, the average pore size increased and more open pores were formed. Degradation studies have shown that all cryogels were resistant to PBS solution for 8 weeks. Cytotoxicity assays demonstrated no toxic effect on viability of rat adipose-derived mesenchymal stem cells (ADMSCs) cultured on cryogels. ADMSC spheroids were proliferated on scaffolds and showed the ability of the cryogels to orient cell differentiation into chondrogenic lineage even in the absence of inductive agents. Thus, our results demonstrate an effective resemblance to extracellular matrix structures specific to cartilage-like microenvironments by cryogels and their further perspective application as potential biomaterials.https://www.mdpi.com/2310-2861/8/9/590cryogelsgelatinhyaluronic acidchondroitin sulfatemesenchymal stem cells |
| spellingShingle | Gulshakhar Kudaibergen Madina Zhunussova Ellina A. Mun Yerlan Ramankulov Vyacheslav Ogay Macroporous Cell-Laden Gelatin/Hyaluronic Acid/Chondroitin Sulfate Cryogels for Engineered Tissue Constructs Gels cryogels gelatin hyaluronic acid chondroitin sulfate mesenchymal stem cells |
| title | Macroporous Cell-Laden Gelatin/Hyaluronic Acid/Chondroitin Sulfate Cryogels for Engineered Tissue Constructs |
| title_full | Macroporous Cell-Laden Gelatin/Hyaluronic Acid/Chondroitin Sulfate Cryogels for Engineered Tissue Constructs |
| title_fullStr | Macroporous Cell-Laden Gelatin/Hyaluronic Acid/Chondroitin Sulfate Cryogels for Engineered Tissue Constructs |
| title_full_unstemmed | Macroporous Cell-Laden Gelatin/Hyaluronic Acid/Chondroitin Sulfate Cryogels for Engineered Tissue Constructs |
| title_short | Macroporous Cell-Laden Gelatin/Hyaluronic Acid/Chondroitin Sulfate Cryogels for Engineered Tissue Constructs |
| title_sort | macroporous cell laden gelatin hyaluronic acid chondroitin sulfate cryogels for engineered tissue constructs |
| topic | cryogels gelatin hyaluronic acid chondroitin sulfate mesenchymal stem cells |
| url | https://www.mdpi.com/2310-2861/8/9/590 |
| work_keys_str_mv | AT gulshakharkudaibergen macroporouscellladengelatinhyaluronicacidchondroitinsulfatecryogelsforengineeredtissueconstructs AT madinazhunussova macroporouscellladengelatinhyaluronicacidchondroitinsulfatecryogelsforengineeredtissueconstructs AT ellinaamun macroporouscellladengelatinhyaluronicacidchondroitinsulfatecryogelsforengineeredtissueconstructs AT yerlanramankulov macroporouscellladengelatinhyaluronicacidchondroitinsulfatecryogelsforengineeredtissueconstructs AT vyacheslavogay macroporouscellladengelatinhyaluronicacidchondroitinsulfatecryogelsforengineeredtissueconstructs |