Global transcriptional response of oral squamous cell carcinoma cell lines to health-associated oral bacteria - an in vitro study
Background We have recently demonstrated that health-associated oral bacteria Streptococcus mitis, Neisseria flavescens, and Haemophilus parainfluenzae induce cytotoxicity in oral squamous cell carcinoma (OSCC) cell lines and downregulate CD36, a cancer-assocaited gene.Aim To explore the effect of t...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Journal of Oral Microbiology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/20002297.2022.2073866 |
| _version_ | 1811235322290241536 |
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| author | Divyashri Baraniya Kumaraswamy Naidu Chitrala Nezar Noor Al-Hebshi |
| author_facet | Divyashri Baraniya Kumaraswamy Naidu Chitrala Nezar Noor Al-Hebshi |
| author_sort | Divyashri Baraniya |
| collection | DOAJ |
| description | Background We have recently demonstrated that health-associated oral bacteria Streptococcus mitis, Neisseria flavescens, and Haemophilus parainfluenzae induce cytotoxicity in oral squamous cell carcinoma (OSCC) cell lines and downregulate CD36, a cancer-assocaited gene.Aim To explore the effect of these three species on global transcriptome of OSCC cell lines.Methods Gene expression of cell lines CAL27, SCC4 and SCC25 cocultured with the test species was assessed with Clariom-S Human microarray. Porphyromonas gingivalis was included as a pathogenic control. Data were analyzed using Ingenuity Pathway Analysis.Results The results differed by species and cell line. Overall, the transcriptional changes by S. mitis were predominantly anti-cancer including inhibition of HOTAIR regulatory pathway, JAK/Stat signaling, cyclins/cyclin-dependent kinases, and endothelin1 signaling. H. parainfluenzae and N. flavescens resulted in a mix of pro- and anti-cancer responses including activation of acute phase response, pro-inflammatory interleukins signaling, TREM-1 signaling, and tumor microenvironment pathway; but downregulation of cell cycle by inhibition of cyclins and cyclin-dependent kinases. P. gingivalis had a predominantly pro-cancer effect limited to SCC4, including upregulation of inflammatory pathways, phospholipases and PI3K signaling.Conclusion These findings provide a new insight into the role of commensal oral bacteria in OSCC. Animal studies are required to further explore them. |
| first_indexed | 2024-04-12T11:48:51Z |
| format | Article |
| id | doaj.art-e9825159c8604b5a98be53918b29ed80 |
| institution | Directory Open Access Journal |
| issn | 2000-2297 |
| language | English |
| last_indexed | 2024-04-12T11:48:51Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj.art-e9825159c8604b5a98be53918b29ed802022-12-22T03:34:15ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972022-12-0114110.1080/20002297.2022.2073866Global transcriptional response of oral squamous cell carcinoma cell lines to health-associated oral bacteria - an in vitro studyDivyashri Baraniya0Kumaraswamy Naidu Chitrala1Nezar Noor Al-Hebshi2Oral Microbiome Research Laboratory, Maurice H. Kornberg School of Dentistry, Temple University, Philadelphia, USAFels Cancer Institute for Personalized Medicine, Lewis Katz School of Medicine, Temple University, Philadelphia, Pennsylvania, USAOral Microbiome Research Laboratory, Maurice H. Kornberg School of Dentistry, Temple University, Philadelphia, USABackground We have recently demonstrated that health-associated oral bacteria Streptococcus mitis, Neisseria flavescens, and Haemophilus parainfluenzae induce cytotoxicity in oral squamous cell carcinoma (OSCC) cell lines and downregulate CD36, a cancer-assocaited gene.Aim To explore the effect of these three species on global transcriptome of OSCC cell lines.Methods Gene expression of cell lines CAL27, SCC4 and SCC25 cocultured with the test species was assessed with Clariom-S Human microarray. Porphyromonas gingivalis was included as a pathogenic control. Data were analyzed using Ingenuity Pathway Analysis.Results The results differed by species and cell line. Overall, the transcriptional changes by S. mitis were predominantly anti-cancer including inhibition of HOTAIR regulatory pathway, JAK/Stat signaling, cyclins/cyclin-dependent kinases, and endothelin1 signaling. H. parainfluenzae and N. flavescens resulted in a mix of pro- and anti-cancer responses including activation of acute phase response, pro-inflammatory interleukins signaling, TREM-1 signaling, and tumor microenvironment pathway; but downregulation of cell cycle by inhibition of cyclins and cyclin-dependent kinases. P. gingivalis had a predominantly pro-cancer effect limited to SCC4, including upregulation of inflammatory pathways, phospholipases and PI3K signaling.Conclusion These findings provide a new insight into the role of commensal oral bacteria in OSCC. Animal studies are required to further explore them.https://www.tandfonline.com/doi/10.1080/20002297.2022.2073866Mouth neoplasmscell linebacteriamicroarray analysistranscriptome |
| spellingShingle | Divyashri Baraniya Kumaraswamy Naidu Chitrala Nezar Noor Al-Hebshi Global transcriptional response of oral squamous cell carcinoma cell lines to health-associated oral bacteria - an in vitro study Journal of Oral Microbiology Mouth neoplasms cell line bacteria microarray analysis transcriptome |
| title | Global transcriptional response of oral squamous cell carcinoma cell lines to health-associated oral bacteria - an in vitro study |
| title_full | Global transcriptional response of oral squamous cell carcinoma cell lines to health-associated oral bacteria - an in vitro study |
| title_fullStr | Global transcriptional response of oral squamous cell carcinoma cell lines to health-associated oral bacteria - an in vitro study |
| title_full_unstemmed | Global transcriptional response of oral squamous cell carcinoma cell lines to health-associated oral bacteria - an in vitro study |
| title_short | Global transcriptional response of oral squamous cell carcinoma cell lines to health-associated oral bacteria - an in vitro study |
| title_sort | global transcriptional response of oral squamous cell carcinoma cell lines to health associated oral bacteria an in vitro study |
| topic | Mouth neoplasms cell line bacteria microarray analysis transcriptome |
| url | https://www.tandfonline.com/doi/10.1080/20002297.2022.2073866 |
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