Genome-wide association mapping in hairy vetch (Vicia villosa) discovers a large effect locus controlling seed dormancy
Hairy vetch (Vicia villosa Roth), a winter-hardy annual legume, is a promising cover crop. To fully leverage its potential, seed production and field performance of V. villosa must be improved to facilitate producer adoption. Two classic domestication traits, seed dormancy (hard seed) and dehiscence...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-10-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1282187/full |
| _version_ | 1797650863609610240 |
|---|---|
| author | Neal Tilhou Lisa Kissing Kucek Brandon Carr Annie Marion Joel Douglas John Englert Shahjahan Ali John Raasch Suresh Bhamidimarri Steven Brian Mirsky Maria J. Monteros Sarah Krogman Ryan Hayes Mark Azevedo Heathcliffe Riday |
| author_facet | Neal Tilhou Lisa Kissing Kucek Brandon Carr Annie Marion Joel Douglas John Englert Shahjahan Ali John Raasch Suresh Bhamidimarri Steven Brian Mirsky Maria J. Monteros Sarah Krogman Ryan Hayes Mark Azevedo Heathcliffe Riday |
| author_sort | Neal Tilhou |
| collection | DOAJ |
| description | Hairy vetch (Vicia villosa Roth), a winter-hardy annual legume, is a promising cover crop. To fully leverage its potential, seed production and field performance of V. villosa must be improved to facilitate producer adoption. Two classic domestication traits, seed dormancy (hard seed) and dehiscence (pod shatter), are selection targets in an ongoing breeding program. This study reports a genome-wide association study of 1,019 V. villosa individuals evaluated at two sites (Knox City, Texas and Corvallis, Oregon) for the proportion of dormant seed, visual pod dehiscence scores, and two dehiscence surrogate measures (force to dehiscence and pod spiraling score). Trait performance varied between sites, but reliability (related to heritability) across sites was strong (dormant seed proportion: 0.68; dehiscence score: 0.61; spiraling score: 0.42; force to dehiscence: 0.41). A major locus controlling seed dormancy was found (q-value: 1.29 × 10−5; chromosome 1: position: 63611165), which can be used by breeding programs to rapidly reduce dormancy in breeding populations. No significant dehiscence score QTL was found, primarily due to the high dehiscence rates in Corvallis, Oregon. Since Oregon is a potentially major V. villosa seed production region, further dehiscence resistance screening is necessary |
| first_indexed | 2024-03-11T16:07:48Z |
| format | Article |
| id | doaj.art-e986fbf0481c4ef6b0e3ada9bb6e29d6 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-11T16:07:48Z |
| publishDate | 2023-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-e986fbf0481c4ef6b0e3ada9bb6e29d62023-10-24T22:12:32ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-10-011410.3389/fpls.2023.12821871282187Genome-wide association mapping in hairy vetch (Vicia villosa) discovers a large effect locus controlling seed dormancyNeal Tilhou0Lisa Kissing Kucek1Brandon Carr2Annie Marion3Joel Douglas4John Englert5Shahjahan Ali6John Raasch7Suresh Bhamidimarri8Steven Brian Mirsky9Maria J. Monteros10Sarah Krogman11Ryan Hayes12Mark Azevedo13Heathcliffe Riday14US Dairy Forage Research Center, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Madison, WI, United StatesUS Dairy Forage Research Center, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Madison, WI, United StatesJames E. “Bud” Smith Plant Materials Center, United States Department of Agriculture-Natural Resources Conservation Service (USDA-NRCS), Knox City, TX, United StatesCorvallis Plant Materials Center, USDA-NRCS, Corvallis, OR, United StatesCentral National Technology Support Center, USDA-NRCS, Fort Worth, TX, United StatesNational Plant Materials Program, USDA-NRCS, Washington, DC, United StatesUS Dairy Forage Research Center, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Madison, WI, United StatesUS Dairy Forage Research Center, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Madison, WI, United StatesCorteva Agriscience, Connell, WA, United StatesSustainable Agricultural Systems Laboratory, United States Department of Agriculture-Natural Resources Conservation Service (USDA-ARS), Beltsville, MD, United StatesBayer Crop Science, Chesterfield, MO, United StatesSchool of Medicine in Kansas, University of Kansas Medical Center, Wichita, KS, United States0Forage Seed and Cereal Research Unit, USDA-ARS, Corvaillis, OR, United States0Forage Seed and Cereal Research Unit, USDA-ARS, Corvaillis, OR, United StatesUS Dairy Forage Research Center, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Madison, WI, United StatesHairy vetch (Vicia villosa Roth), a winter-hardy annual legume, is a promising cover crop. To fully leverage its potential, seed production and field performance of V. villosa must be improved to facilitate producer adoption. Two classic domestication traits, seed dormancy (hard seed) and dehiscence (pod shatter), are selection targets in an ongoing breeding program. This study reports a genome-wide association study of 1,019 V. villosa individuals evaluated at two sites (Knox City, Texas and Corvallis, Oregon) for the proportion of dormant seed, visual pod dehiscence scores, and two dehiscence surrogate measures (force to dehiscence and pod spiraling score). Trait performance varied between sites, but reliability (related to heritability) across sites was strong (dormant seed proportion: 0.68; dehiscence score: 0.61; spiraling score: 0.42; force to dehiscence: 0.41). A major locus controlling seed dormancy was found (q-value: 1.29 × 10−5; chromosome 1: position: 63611165), which can be used by breeding programs to rapidly reduce dormancy in breeding populations. No significant dehiscence score QTL was found, primarily due to the high dehiscence rates in Corvallis, Oregon. Since Oregon is a potentially major V. villosa seed production region, further dehiscence resistance screening is necessaryhttps://www.frontiersin.org/articles/10.3389/fpls.2023.1282187/fullseed dormancygenomewide associationcover croplegumegenomics |
| spellingShingle | Neal Tilhou Lisa Kissing Kucek Brandon Carr Annie Marion Joel Douglas John Englert Shahjahan Ali John Raasch Suresh Bhamidimarri Steven Brian Mirsky Maria J. Monteros Sarah Krogman Ryan Hayes Mark Azevedo Heathcliffe Riday Genome-wide association mapping in hairy vetch (Vicia villosa) discovers a large effect locus controlling seed dormancy Frontiers in Plant Science seed dormancy genomewide association cover crop legume genomics |
| title | Genome-wide association mapping in hairy vetch (Vicia villosa) discovers a large effect locus controlling seed dormancy |
| title_full | Genome-wide association mapping in hairy vetch (Vicia villosa) discovers a large effect locus controlling seed dormancy |
| title_fullStr | Genome-wide association mapping in hairy vetch (Vicia villosa) discovers a large effect locus controlling seed dormancy |
| title_full_unstemmed | Genome-wide association mapping in hairy vetch (Vicia villosa) discovers a large effect locus controlling seed dormancy |
| title_short | Genome-wide association mapping in hairy vetch (Vicia villosa) discovers a large effect locus controlling seed dormancy |
| title_sort | genome wide association mapping in hairy vetch vicia villosa discovers a large effect locus controlling seed dormancy |
| topic | seed dormancy genomewide association cover crop legume genomics |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1282187/full |
| work_keys_str_mv | AT nealtilhou genomewideassociationmappinginhairyvetchviciavillosadiscoversalargeeffectlocuscontrollingseeddormancy AT lisakissingkucek genomewideassociationmappinginhairyvetchviciavillosadiscoversalargeeffectlocuscontrollingseeddormancy AT brandoncarr genomewideassociationmappinginhairyvetchviciavillosadiscoversalargeeffectlocuscontrollingseeddormancy AT anniemarion genomewideassociationmappinginhairyvetchviciavillosadiscoversalargeeffectlocuscontrollingseeddormancy AT joeldouglas genomewideassociationmappinginhairyvetchviciavillosadiscoversalargeeffectlocuscontrollingseeddormancy AT johnenglert genomewideassociationmappinginhairyvetchviciavillosadiscoversalargeeffectlocuscontrollingseeddormancy AT shahjahanali genomewideassociationmappinginhairyvetchviciavillosadiscoversalargeeffectlocuscontrollingseeddormancy AT johnraasch genomewideassociationmappinginhairyvetchviciavillosadiscoversalargeeffectlocuscontrollingseeddormancy AT sureshbhamidimarri genomewideassociationmappinginhairyvetchviciavillosadiscoversalargeeffectlocuscontrollingseeddormancy AT stevenbrianmirsky genomewideassociationmappinginhairyvetchviciavillosadiscoversalargeeffectlocuscontrollingseeddormancy AT mariajmonteros genomewideassociationmappinginhairyvetchviciavillosadiscoversalargeeffectlocuscontrollingseeddormancy AT sarahkrogman genomewideassociationmappinginhairyvetchviciavillosadiscoversalargeeffectlocuscontrollingseeddormancy AT ryanhayes genomewideassociationmappinginhairyvetchviciavillosadiscoversalargeeffectlocuscontrollingseeddormancy AT markazevedo genomewideassociationmappinginhairyvetchviciavillosadiscoversalargeeffectlocuscontrollingseeddormancy AT heathclifferiday genomewideassociationmappinginhairyvetchviciavillosadiscoversalargeeffectlocuscontrollingseeddormancy |