Macrosight: A Novel Framework to Analyze the Shape and Movement of Interacting Macrophages Using Matlab®
This paper presents a novel software framework, called macrosight, which incorporates routines to detect, track, and analyze the shape and movement of objects, with special emphasis on macrophages. The key feature presented in macrosight consists of an algorithm to assess the changes of direction de...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-01-01
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| Series: | Journal of Imaging |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2313-433X/5/1/17 |
| _version_ | 1818533087816974336 |
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| author | José Alonso Solís-Lemus Brian Stramer Greg Slabaugh Constantino Carlos Reyes-Aldasoro |
| author_facet | José Alonso Solís-Lemus Brian Stramer Greg Slabaugh Constantino Carlos Reyes-Aldasoro |
| author_sort | José Alonso Solís-Lemus |
| collection | DOAJ |
| description | This paper presents a novel software framework, called macrosight, which incorporates routines to detect, track, and analyze the shape and movement of objects, with special emphasis on macrophages. The key feature presented in macrosight consists of an algorithm to assess the changes of direction derived from cell–cell contact, where an interaction is assumed to occur. The main biological motivation is the determination of certain cell interactions influencing cell migration. Thus, the main objective of this work is to provide insights into the notion that interactions between cell structures cause a change in orientation. Macrosight analyzes the change of direction of cells before and after they come in contact with another cell. Interactions are determined when the cells overlap and form clumps of two or more cells. The framework integrates a segmentation technique capable of detecting overlapping cells and a tracking framework into a tool for the analysis of the trajectories of cells before and after they overlap. Preliminary results show promise into the analysis and the hypothesis proposed, and lays the groundwork for further developments. The extensive experimentation and data analysis show, with statistical significance, that under certain conditions, the movement changes before and after an interaction are different from movement in controlled cases. |
| first_indexed | 2024-12-11T17:54:03Z |
| format | Article |
| id | doaj.art-e987ce127458445cae3d984a1000ed4e |
| institution | Directory Open Access Journal |
| issn | 2313-433X |
| language | English |
| last_indexed | 2024-12-11T17:54:03Z |
| publishDate | 2019-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Imaging |
| spelling | doaj.art-e987ce127458445cae3d984a1000ed4e2022-12-22T00:56:08ZengMDPI AGJournal of Imaging2313-433X2019-01-01511710.3390/jimaging5010017jimaging5010017Macrosight: A Novel Framework to Analyze the Shape and Movement of Interacting Macrophages Using Matlab®José Alonso Solís-Lemus0Brian Stramer1Greg Slabaugh2Constantino Carlos Reyes-Aldasoro3School of Mathematics, Computer Science and Engineering, City, University of London, London EC1V 0HB, UKRandall Division of Cell & Molecular Biophysics, King’s College London, London WC2R 2LS, UKSchool of Mathematics, Computer Science and Engineering, City, University of London, London EC1V 0HB, UKSchool of Mathematics, Computer Science and Engineering, City, University of London, London EC1V 0HB, UKThis paper presents a novel software framework, called macrosight, which incorporates routines to detect, track, and analyze the shape and movement of objects, with special emphasis on macrophages. The key feature presented in macrosight consists of an algorithm to assess the changes of direction derived from cell–cell contact, where an interaction is assumed to occur. The main biological motivation is the determination of certain cell interactions influencing cell migration. Thus, the main objective of this work is to provide insights into the notion that interactions between cell structures cause a change in orientation. Macrosight analyzes the change of direction of cells before and after they come in contact with another cell. Interactions are determined when the cells overlap and form clumps of two or more cells. The framework integrates a segmentation technique capable of detecting overlapping cells and a tracking framework into a tool for the analysis of the trajectories of cells before and after they overlap. Preliminary results show promise into the analysis and the hypothesis proposed, and lays the groundwork for further developments. The extensive experimentation and data analysis show, with statistical significance, that under certain conditions, the movement changes before and after an interaction are different from movement in controlled cases.http://www.mdpi.com/2313-433X/5/1/17segmentationmacrophagesoverlapping objectsshape analysismovement analysis |
| spellingShingle | José Alonso Solís-Lemus Brian Stramer Greg Slabaugh Constantino Carlos Reyes-Aldasoro Macrosight: A Novel Framework to Analyze the Shape and Movement of Interacting Macrophages Using Matlab® Journal of Imaging segmentation macrophages overlapping objects shape analysis movement analysis |
| title | Macrosight: A Novel Framework to Analyze the Shape and Movement of Interacting Macrophages Using Matlab® |
| title_full | Macrosight: A Novel Framework to Analyze the Shape and Movement of Interacting Macrophages Using Matlab® |
| title_fullStr | Macrosight: A Novel Framework to Analyze the Shape and Movement of Interacting Macrophages Using Matlab® |
| title_full_unstemmed | Macrosight: A Novel Framework to Analyze the Shape and Movement of Interacting Macrophages Using Matlab® |
| title_short | Macrosight: A Novel Framework to Analyze the Shape and Movement of Interacting Macrophages Using Matlab® |
| title_sort | macrosight a novel framework to analyze the shape and movement of interacting macrophages using matlab r |
| topic | segmentation macrophages overlapping objects shape analysis movement analysis |
| url | http://www.mdpi.com/2313-433X/5/1/17 |
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