Differential mechanisms underlying responses of soil bacterial and fungal communities to nitrogen and phosphorus inputs in a subtropical forest
Atmospheric nitrogen (N) deposition and phosphorus (P) addition both can change soil bacterial and fungal community structure with a consequent impact on ecosystem functions. However, which factor plays an important role in regulating responses of bacterial and fungal community to N and P enrichment...
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PeerJ Inc.
2019-09-01
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| author | Yong Li Dashuan Tian Jinsong Wang Shuli Niu Jing Tian Denglong Ha Yuxi Qu Guangwei Jing Xiaoming Kang Bing Song |
| author_facet | Yong Li Dashuan Tian Jinsong Wang Shuli Niu Jing Tian Denglong Ha Yuxi Qu Guangwei Jing Xiaoming Kang Bing Song |
| author_sort | Yong Li |
| collection | DOAJ |
| description | Atmospheric nitrogen (N) deposition and phosphorus (P) addition both can change soil bacterial and fungal community structure with a consequent impact on ecosystem functions. However, which factor plays an important role in regulating responses of bacterial and fungal community to N and P enrichments remains unclear. We conducted a manipulative experiment to simulate N and P inputs (10 g N · m−2 · yr−1 NH4NO3 or 10 g P · m−2 · yr−1 NaH2PO4) and compared their effects on soil bacterial and fungal species richness and community composition. The results showed that the addition of N significantly increased NH4+ and Al3+ by 99.6% and 57.4%, respectively, and consequently led to a decline in soil pH from 4.18 to 3.75 after a 5-year treatment. P addition increased Al3+ and available P by 27.0% and 10-fold, respectively, but had no effect on soil pH. N addition significantly decreased bacterial species richness and Shannon index and resulted in a substantial shift of bacterial community composition, whereas P addition did not. Neither N nor P addition changed fungal species richness, Shannon index, and fungal community composition. A structural equation model showed that the shift in bacterial community composition was related to an increase in soil acid cations. The principal component scores of soil nutrients showed a significantly positive relationship with fungal community composition. Our results suggest that N and P additions affect soil bacterial and fungal communities in different ways in subtropical forest. These findings highlight how the diversity of microbial communities of subtropical forest soil will depend on future scenarios of anthropogenic N deposition and P enrichment, with a particular sensitivity of bacterial community to N addition. |
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| language | English |
| last_indexed | 2024-03-09T07:54:05Z |
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| spelling | doaj.art-e989e721496243779285579cb40047cb2023-12-03T01:22:15ZengPeerJ Inc.PeerJ2167-83592019-09-017e763110.7717/peerj.7631Differential mechanisms underlying responses of soil bacterial and fungal communities to nitrogen and phosphorus inputs in a subtropical forestYong Li0Dashuan Tian1Jinsong Wang2Shuli Niu3Jing Tian4Denglong Ha5Yuxi Qu6Guangwei Jing7Xiaoming Kang8Bing Song9Beijing Key Laboratory of Wetland Services and Restoration, Institute of Wetland Research, Chinese Academy of Forestry, Beijing, ChinaKey Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Beijing, ChinaKey Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Beijing, ChinaKey Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Beijing, ChinaKey Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Beijing, ChinaJigongshan Natural Reserve, Xinyang, ChinaJigongshan Natural Reserve, Xinyang, ChinaJigongshan Natural Reserve, Xinyang, ChinaBeijing Key Laboratory of Wetland Services and Restoration, Institute of Wetland Research, Chinese Academy of Forestry, Beijing, ChinaSchool of Resources and Environmental Engineering, Ludong University, Yantai, ChinaAtmospheric nitrogen (N) deposition and phosphorus (P) addition both can change soil bacterial and fungal community structure with a consequent impact on ecosystem functions. However, which factor plays an important role in regulating responses of bacterial and fungal community to N and P enrichments remains unclear. We conducted a manipulative experiment to simulate N and P inputs (10 g N · m−2 · yr−1 NH4NO3 or 10 g P · m−2 · yr−1 NaH2PO4) and compared their effects on soil bacterial and fungal species richness and community composition. The results showed that the addition of N significantly increased NH4+ and Al3+ by 99.6% and 57.4%, respectively, and consequently led to a decline in soil pH from 4.18 to 3.75 after a 5-year treatment. P addition increased Al3+ and available P by 27.0% and 10-fold, respectively, but had no effect on soil pH. N addition significantly decreased bacterial species richness and Shannon index and resulted in a substantial shift of bacterial community composition, whereas P addition did not. Neither N nor P addition changed fungal species richness, Shannon index, and fungal community composition. A structural equation model showed that the shift in bacterial community composition was related to an increase in soil acid cations. The principal component scores of soil nutrients showed a significantly positive relationship with fungal community composition. Our results suggest that N and P additions affect soil bacterial and fungal communities in different ways in subtropical forest. These findings highlight how the diversity of microbial communities of subtropical forest soil will depend on future scenarios of anthropogenic N deposition and P enrichment, with a particular sensitivity of bacterial community to N addition.https://peerj.com/articles/7631.pdfNitrogen depositionPhosphorus additionMicrobial diversityCommunity compositionAmplicon sequencingSubtropical forest |
| spellingShingle | Yong Li Dashuan Tian Jinsong Wang Shuli Niu Jing Tian Denglong Ha Yuxi Qu Guangwei Jing Xiaoming Kang Bing Song Differential mechanisms underlying responses of soil bacterial and fungal communities to nitrogen and phosphorus inputs in a subtropical forest PeerJ Nitrogen deposition Phosphorus addition Microbial diversity Community composition Amplicon sequencing Subtropical forest |
| title | Differential mechanisms underlying responses of soil bacterial and fungal communities to nitrogen and phosphorus inputs in a subtropical forest |
| title_full | Differential mechanisms underlying responses of soil bacterial and fungal communities to nitrogen and phosphorus inputs in a subtropical forest |
| title_fullStr | Differential mechanisms underlying responses of soil bacterial and fungal communities to nitrogen and phosphorus inputs in a subtropical forest |
| title_full_unstemmed | Differential mechanisms underlying responses of soil bacterial and fungal communities to nitrogen and phosphorus inputs in a subtropical forest |
| title_short | Differential mechanisms underlying responses of soil bacterial and fungal communities to nitrogen and phosphorus inputs in a subtropical forest |
| title_sort | differential mechanisms underlying responses of soil bacterial and fungal communities to nitrogen and phosphorus inputs in a subtropical forest |
| topic | Nitrogen deposition Phosphorus addition Microbial diversity Community composition Amplicon sequencing Subtropical forest |
| url | https://peerj.com/articles/7631.pdf |
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