Establishment of enzyme-linked immunosorbent facilitated antigen binding as a biomarker assay for Japanese cedar pollen allergy immunotherapy
Background: Clinical efficacy of allergen-specific Immunotherapy (AIT) towards Japanese cedar (JC) pollen allergy is firmly established but JC pollen-specific biomarker assays are lacking. Treatment-related increase of allergen-specific antibodies is a robust biomarker of successful AIT. Allergen-sp...
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| Format: | Article |
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Elsevier
2019-07-01
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| Series: | Journal of Pharmacological Sciences |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1347861319356762 |
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| author | Chiharu Fukano Katsuyo Ohashi-Doi Kaare Lund Atsuhito Nakao Keisuke Masuyama Tomokazu Matsuoka |
| author_facet | Chiharu Fukano Katsuyo Ohashi-Doi Kaare Lund Atsuhito Nakao Keisuke Masuyama Tomokazu Matsuoka |
| author_sort | Chiharu Fukano |
| collection | DOAJ |
| description | Background: Clinical efficacy of allergen-specific Immunotherapy (AIT) towards Japanese cedar (JC) pollen allergy is firmly established but JC pollen-specific biomarker assays are lacking. Treatment-related increase of allergen-specific antibodies is a robust biomarker of successful AIT. Allergen-specific non-IgE antibodies are believed to reduce the effects of allergen exposure by competing with IgE for allergen binding, and in-vitro assays quantifying the effects of AIT-induced IgE-blocking antibodies are advantageous. A cell-free enzyme-linked immunosorbent facilitated antigen binding (ELIFAB) assay of JC pollen was established. Methods: Serum IgE–allergen complexes were captured by immobilized recombinant CD23, and allergen–IgE–CD23 complexes were detected by a biotin-conjugated anti-human IgE antibody. Sera from JC pollen-allergic subjects without or with subcutaneous immunotherapy (SCIT) with JC pollen extract were used (n = 11/group). Results: Optimal assay conditions were established at 20 μg/mL CD23 and 0.3 μg/mL JC pollen extract, and the dependency on CD23 and IgE was verified. The data show that the JC pollen ELIFAB assay is fit for purpose and demonstrates that the IgE-blocking activity is significantly increased in the JC pollen SCIT group compared with the non-treated group. Conclusion: The JC pollen ELIFAB assay represents a simple, cell-free biomarker assay for monitoring the development of IgE-blocking antibody activity during JC pollen AIT. Keywords: Allergy immunotherapy, Allergic rhinitis, IgE-facilitated allergen presentation, Japanese cedar pollen, Subcutaneous immunotherapy |
| first_indexed | 2024-12-16T16:11:39Z |
| format | Article |
| id | doaj.art-e98a28205f894e88be8633295c7bdc8d |
| institution | Directory Open Access Journal |
| issn | 1347-8613 |
| language | English |
| last_indexed | 2024-12-16T16:11:39Z |
| publishDate | 2019-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmacological Sciences |
| spelling | doaj.art-e98a28205f894e88be8633295c7bdc8d2022-12-21T22:25:13ZengElsevierJournal of Pharmacological Sciences1347-86132019-07-011403223227Establishment of enzyme-linked immunosorbent facilitated antigen binding as a biomarker assay for Japanese cedar pollen allergy immunotherapyChiharu Fukano0Katsuyo Ohashi-Doi1Kaare Lund2Atsuhito Nakao3Keisuke Masuyama4Tomokazu Matsuoka5Research Laboratory, Torii Pharmaceutical Co., Ltd., 2183-1 Ota Teranosaku, Sakura-shi, Chiba, Japan; Department of Immunology, Faculty of Medicine, University of Yamanashi, 1110 Shimokato, Chuo-shi, Yamanashi, JapanResearch Laboratory, Torii Pharmaceutical Co., Ltd., 2183-1 Ota Teranosaku, Sakura-shi, Chiba, Japan; Corresponding author. Research Laboratory, Torii Pharmaceutical Co., Ltd., 2183-1 Ota Teranosaku, Sakura-shi, Chiba, 285-0808, Japan. Fax: +81 43 485 5989.Papermill Medical, Ole Maaløes Vej 3, DK-2200 Copenhagen N, DenmarkDepartment of Immunology, Faculty of Medicine, University of Yamanashi, 1110 Shimokato, Chuo-shi, Yamanashi, JapanDepartment of Otorhinolaryngology, Head and Neck Surgery, Faculty of Medicine, University of Yamanashi, 1110 Shimokato, Chuo-shi, Yamanashi, JapanDepartment of Otorhinolaryngology, Head and Neck Surgery, Faculty of Medicine, University of Yamanashi, 1110 Shimokato, Chuo-shi, Yamanashi, JapanBackground: Clinical efficacy of allergen-specific Immunotherapy (AIT) towards Japanese cedar (JC) pollen allergy is firmly established but JC pollen-specific biomarker assays are lacking. Treatment-related increase of allergen-specific antibodies is a robust biomarker of successful AIT. Allergen-specific non-IgE antibodies are believed to reduce the effects of allergen exposure by competing with IgE for allergen binding, and in-vitro assays quantifying the effects of AIT-induced IgE-blocking antibodies are advantageous. A cell-free enzyme-linked immunosorbent facilitated antigen binding (ELIFAB) assay of JC pollen was established. Methods: Serum IgE–allergen complexes were captured by immobilized recombinant CD23, and allergen–IgE–CD23 complexes were detected by a biotin-conjugated anti-human IgE antibody. Sera from JC pollen-allergic subjects without or with subcutaneous immunotherapy (SCIT) with JC pollen extract were used (n = 11/group). Results: Optimal assay conditions were established at 20 μg/mL CD23 and 0.3 μg/mL JC pollen extract, and the dependency on CD23 and IgE was verified. The data show that the JC pollen ELIFAB assay is fit for purpose and demonstrates that the IgE-blocking activity is significantly increased in the JC pollen SCIT group compared with the non-treated group. Conclusion: The JC pollen ELIFAB assay represents a simple, cell-free biomarker assay for monitoring the development of IgE-blocking antibody activity during JC pollen AIT. Keywords: Allergy immunotherapy, Allergic rhinitis, IgE-facilitated allergen presentation, Japanese cedar pollen, Subcutaneous immunotherapyhttp://www.sciencedirect.com/science/article/pii/S1347861319356762 |
| spellingShingle | Chiharu Fukano Katsuyo Ohashi-Doi Kaare Lund Atsuhito Nakao Keisuke Masuyama Tomokazu Matsuoka Establishment of enzyme-linked immunosorbent facilitated antigen binding as a biomarker assay for Japanese cedar pollen allergy immunotherapy Journal of Pharmacological Sciences |
| title | Establishment of enzyme-linked immunosorbent facilitated antigen binding as a biomarker assay for Japanese cedar pollen allergy immunotherapy |
| title_full | Establishment of enzyme-linked immunosorbent facilitated antigen binding as a biomarker assay for Japanese cedar pollen allergy immunotherapy |
| title_fullStr | Establishment of enzyme-linked immunosorbent facilitated antigen binding as a biomarker assay for Japanese cedar pollen allergy immunotherapy |
| title_full_unstemmed | Establishment of enzyme-linked immunosorbent facilitated antigen binding as a biomarker assay for Japanese cedar pollen allergy immunotherapy |
| title_short | Establishment of enzyme-linked immunosorbent facilitated antigen binding as a biomarker assay for Japanese cedar pollen allergy immunotherapy |
| title_sort | establishment of enzyme linked immunosorbent facilitated antigen binding as a biomarker assay for japanese cedar pollen allergy immunotherapy |
| url | http://www.sciencedirect.com/science/article/pii/S1347861319356762 |
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