The effect of dietary supplementation of medium-chain fatty acids products on gut and hepatopancreas health, and disease resistance in white shrimp (Litopenaeus vannamei)
This study was to investigate the effect of medium-chain fatty acids products Aromabiotic® Shrimp (ABS) on growth, gut and hepatopancreas health, gut microbiota and resistance to bacterial infection and adverse environment of white shrimp. Firstly, white shrimp were divided into five groups: control...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513423000200 |
| _version_ | 1797862146212626432 |
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| author | Wei Zhou Yadong Xie Mingxu Xie Hui Liang Ming Li Bingfei Zhou Chao Ran Zhigang Zhou |
| author_facet | Wei Zhou Yadong Xie Mingxu Xie Hui Liang Ming Li Bingfei Zhou Chao Ran Zhigang Zhou |
| author_sort | Wei Zhou |
| collection | DOAJ |
| description | This study was to investigate the effect of medium-chain fatty acids products Aromabiotic® Shrimp (ABS) on growth, gut and hepatopancreas health, gut microbiota and resistance to bacterial infection and adverse environment of white shrimp. Firstly, white shrimp were divided into five groups: control, ABS0.5, ABS1, ABS2, and ABS5 (with 0, 0.5, 1, 2 and 5 g/kg ABS in feed respectively). After 5-week feeding, there was no significant difference in weight gain, feed conversion ratio and survival among groups. However, ABS diets significantly improved the survival of the white shrimp after Vibrio parahaemolyticus challenge (ABS5, P < 0.05) and moldy diet feeding (ABS1 and ABS5, P < 0.05). On this basis, we selected the control, ABS1 and ABS5 groups for the second batch of feeding experiments. After 5-weeks feeding, the level of lipopolysaccharide binding protein (LBP) in intestinal tissue decreased significantly in ABS1 and ABS5 groups versus control (P < 0.05), while the intestinal expression of prophenoloxidase (proPO) and antimicrobial peptides (Crustin and Penaeidin-3a) significantly increased (P < 0.05). The expression of intestinal phenoloxidase (PO) in ABS5 group increased significantly (P < 0.01). The gut microbiota of ABS1 and ABS5 groups changed significantly, and the diversity of gut microbiota decreased. The histological structure of hepatopancreas in ABS1 and ABS5 groups was improved compared with control. The arrangement of hepatopancreas was more compact, and the structure of star-shaped lumen was more obvious. The level of glutamic pyruvic transaminase (ALT) in muscle tissue of ABS1 and ABS5 groups decreased significantly (P < 0.01). Furthermore, the expression of Lysozyme, Crustin and proPO in hepatopancreas was increased in both ABS groups versus control. Collectively, our results showed beneficial effects of dietary supplementation of ABS on gut and hepatopancreas health and disease resistance of white shrimp culture. |
| first_indexed | 2024-04-09T22:15:39Z |
| format | Article |
| id | doaj.art-e98b8cb1a56e42989b3b38c88bf448c1 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-04-09T22:15:39Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-e98b8cb1a56e42989b3b38c88bf448c12023-03-23T04:36:17ZengElsevierAquaculture Reports2352-51342023-04-0129101481The effect of dietary supplementation of medium-chain fatty acids products on gut and hepatopancreas health, and disease resistance in white shrimp (Litopenaeus vannamei)Wei Zhou0Yadong Xie1Mingxu Xie2Hui Liang3Ming Li4Bingfei Zhou5Chao Ran6Zhigang Zhou7Sino-Norway Joint Lab on Fish Gut Microbiota, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaSino-Norway Joint Lab on Fish Gut Microbiota, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaSino-Norway Joint Lab on Fish Gut Microbiota, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaSino-Norway Joint Lab on Fish Gut Microbiota, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaSino-Norway Joint Lab on Fish Gut Microbiota, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaRoyal Agrifirm Group China, Shanghai 200120, ChinaKey Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Corresponding authors.Sino-Norway Joint Lab on Fish Gut Microbiota, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Corresponding authors.This study was to investigate the effect of medium-chain fatty acids products Aromabiotic® Shrimp (ABS) on growth, gut and hepatopancreas health, gut microbiota and resistance to bacterial infection and adverse environment of white shrimp. Firstly, white shrimp were divided into five groups: control, ABS0.5, ABS1, ABS2, and ABS5 (with 0, 0.5, 1, 2 and 5 g/kg ABS in feed respectively). After 5-week feeding, there was no significant difference in weight gain, feed conversion ratio and survival among groups. However, ABS diets significantly improved the survival of the white shrimp after Vibrio parahaemolyticus challenge (ABS5, P < 0.05) and moldy diet feeding (ABS1 and ABS5, P < 0.05). On this basis, we selected the control, ABS1 and ABS5 groups for the second batch of feeding experiments. After 5-weeks feeding, the level of lipopolysaccharide binding protein (LBP) in intestinal tissue decreased significantly in ABS1 and ABS5 groups versus control (P < 0.05), while the intestinal expression of prophenoloxidase (proPO) and antimicrobial peptides (Crustin and Penaeidin-3a) significantly increased (P < 0.05). The expression of intestinal phenoloxidase (PO) in ABS5 group increased significantly (P < 0.01). The gut microbiota of ABS1 and ABS5 groups changed significantly, and the diversity of gut microbiota decreased. The histological structure of hepatopancreas in ABS1 and ABS5 groups was improved compared with control. The arrangement of hepatopancreas was more compact, and the structure of star-shaped lumen was more obvious. The level of glutamic pyruvic transaminase (ALT) in muscle tissue of ABS1 and ABS5 groups decreased significantly (P < 0.01). Furthermore, the expression of Lysozyme, Crustin and proPO in hepatopancreas was increased in both ABS groups versus control. Collectively, our results showed beneficial effects of dietary supplementation of ABS on gut and hepatopancreas health and disease resistance of white shrimp culture.http://www.sciencedirect.com/science/article/pii/S2352513423000200Litopenaeus vannameiGut healthGut microbiotaHepatopancreas healthMedium-chain fatty acids |
| spellingShingle | Wei Zhou Yadong Xie Mingxu Xie Hui Liang Ming Li Bingfei Zhou Chao Ran Zhigang Zhou The effect of dietary supplementation of medium-chain fatty acids products on gut and hepatopancreas health, and disease resistance in white shrimp (Litopenaeus vannamei) Aquaculture Reports Litopenaeus vannamei Gut health Gut microbiota Hepatopancreas health Medium-chain fatty acids |
| title | The effect of dietary supplementation of medium-chain fatty acids products on gut and hepatopancreas health, and disease resistance in white shrimp (Litopenaeus vannamei) |
| title_full | The effect of dietary supplementation of medium-chain fatty acids products on gut and hepatopancreas health, and disease resistance in white shrimp (Litopenaeus vannamei) |
| title_fullStr | The effect of dietary supplementation of medium-chain fatty acids products on gut and hepatopancreas health, and disease resistance in white shrimp (Litopenaeus vannamei) |
| title_full_unstemmed | The effect of dietary supplementation of medium-chain fatty acids products on gut and hepatopancreas health, and disease resistance in white shrimp (Litopenaeus vannamei) |
| title_short | The effect of dietary supplementation of medium-chain fatty acids products on gut and hepatopancreas health, and disease resistance in white shrimp (Litopenaeus vannamei) |
| title_sort | effect of dietary supplementation of medium chain fatty acids products on gut and hepatopancreas health and disease resistance in white shrimp litopenaeus vannamei |
| topic | Litopenaeus vannamei Gut health Gut microbiota Hepatopancreas health Medium-chain fatty acids |
| url | http://www.sciencedirect.com/science/article/pii/S2352513423000200 |
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