Blood levels of pro-inflammatory and anti-inflammatory cytokines during an oral glucose tolerance test in patients with symptoms suggesting reactive hypoglycemia
We evaluated the impact of postprandial glycemia on blood levels of pro-inflammatory and anti-inflammatory cytokines during an oral glucose tolerance test in non-diabetic patients with symptoms suggesting reactive hypoglycemia. Eleven patients with clinical symptoms suggesting reactive hypoglycemia...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Associação Brasileira de Divulgação Científica
2016-01-01
|
| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000800702&lng=en&tlng=en |
| _version_ | 1819154215551893504 |
|---|---|
| author | W. Eik Filho S.S. Marcon T. Krupek I.T.S. Previdelli O.C.N. Pereira M.A.R.C.P. Silva R.B. Bazotte |
| author_facet | W. Eik Filho S.S. Marcon T. Krupek I.T.S. Previdelli O.C.N. Pereira M.A.R.C.P. Silva R.B. Bazotte |
| author_sort | W. Eik Filho |
| collection | DOAJ |
| description | We evaluated the impact of postprandial glycemia on blood levels of pro-inflammatory and anti-inflammatory cytokines during an oral glucose tolerance test in non-diabetic patients with symptoms suggesting reactive hypoglycemia. Eleven patients with clinical symptoms suggesting reactive hypoglycemia received an oral glucose solution (75 g) Blood was collected at 0 (baseline), 30, 60, 120 and 180 min after glucose ingestion and the plasma concentrations of interferon-α (IFN-α), interferon-γ (IFN-γ), interleukin-1 receptor antagonist (IL-1RA), interleukin 2 (IL-2), interleukin-2 receptor (IL-2R), interleukin 4 (IL-4), interleukin 6 (IL-6), interleukin 8 (IL-8), interleukin 10 (IL-10), interleukin-12 (IL-12), interleukin 13 (IL-13), interleukin 15 (IL-15), interleukin 17 (IL-17), IFN-γ inducible protein 10 (IP-10), monocyte chemotactic protein 1 (MCP1), monokine induced by IFN-γ (MIG), macrophage inflammatory protein-1α (MIP-1α), interleukin-1β (IL-1β), colony stimulating factor (G-CSF), granulocyte-macrophage CSF (GM-CSF), basic fibroblast growth factor (FGF-basic), eotaxin, tumor necrosis factor α (TNFα), epidermal growth factor (EGF), hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF), macrophage inflammatory protein-1α (MIP-1α), and 1β (MIP-1β) were evaluated. Overall, glycemic levels increased, reached its maximum at 30 min (phase 1), returned to baseline levels at 120 min (phase 2), followed by a mild hypoglycemia at 180 min (phase 3). During phase 1, cytokine blood levels were maintained. However, we observed a synchronous fall (P<0.05) in the concentrations of pro-inflammatory (IL-15, IL-17, MCP-1) and anti-inflammatory cytokines (FGF-basic, IL-13, IL-1RA) during phase 2. Furthermore, a simultaneous rise (P<0.05) of pro-inflammatory (IL-2, IL-5, IL-17) and anti-inflammatory cytokines (IL-4, IL-1RA, IL-2R, IL-13, FGF-basic) occurred during phase 3. Thus, mild acute hypoglycemia but not a physiological increase of glycemia was associated with increased blood levels of anti-inflammatory and pro-inflammatory cytokines. |
| first_indexed | 2024-12-22T15:17:32Z |
| format | Article |
| id | doaj.art-e98ece603e43424c94f20025d3fa6925 |
| institution | Directory Open Access Journal |
| issn | 1414-431X |
| language | English |
| last_indexed | 2024-12-22T15:17:32Z |
| publishDate | 2016-01-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-e98ece603e43424c94f20025d3fa69252022-12-21T18:21:44ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research1414-431X2016-01-0149810.1590/1414-431X20165195S0100-879X2016000800702Blood levels of pro-inflammatory and anti-inflammatory cytokines during an oral glucose tolerance test in patients with symptoms suggesting reactive hypoglycemiaW. Eik FilhoS.S. MarconT. KrupekI.T.S. PrevidelliO.C.N. PereiraM.A.R.C.P. SilvaR.B. BazotteWe evaluated the impact of postprandial glycemia on blood levels of pro-inflammatory and anti-inflammatory cytokines during an oral glucose tolerance test in non-diabetic patients with symptoms suggesting reactive hypoglycemia. Eleven patients with clinical symptoms suggesting reactive hypoglycemia received an oral glucose solution (75 g) Blood was collected at 0 (baseline), 30, 60, 120 and 180 min after glucose ingestion and the plasma concentrations of interferon-α (IFN-α), interferon-γ (IFN-γ), interleukin-1 receptor antagonist (IL-1RA), interleukin 2 (IL-2), interleukin-2 receptor (IL-2R), interleukin 4 (IL-4), interleukin 6 (IL-6), interleukin 8 (IL-8), interleukin 10 (IL-10), interleukin-12 (IL-12), interleukin 13 (IL-13), interleukin 15 (IL-15), interleukin 17 (IL-17), IFN-γ inducible protein 10 (IP-10), monocyte chemotactic protein 1 (MCP1), monokine induced by IFN-γ (MIG), macrophage inflammatory protein-1α (MIP-1α), interleukin-1β (IL-1β), colony stimulating factor (G-CSF), granulocyte-macrophage CSF (GM-CSF), basic fibroblast growth factor (FGF-basic), eotaxin, tumor necrosis factor α (TNFα), epidermal growth factor (EGF), hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF), macrophage inflammatory protein-1α (MIP-1α), and 1β (MIP-1β) were evaluated. Overall, glycemic levels increased, reached its maximum at 30 min (phase 1), returned to baseline levels at 120 min (phase 2), followed by a mild hypoglycemia at 180 min (phase 3). During phase 1, cytokine blood levels were maintained. However, we observed a synchronous fall (P<0.05) in the concentrations of pro-inflammatory (IL-15, IL-17, MCP-1) and anti-inflammatory cytokines (FGF-basic, IL-13, IL-1RA) during phase 2. Furthermore, a simultaneous rise (P<0.05) of pro-inflammatory (IL-2, IL-5, IL-17) and anti-inflammatory cytokines (IL-4, IL-1RA, IL-2R, IL-13, FGF-basic) occurred during phase 3. Thus, mild acute hypoglycemia but not a physiological increase of glycemia was associated with increased blood levels of anti-inflammatory and pro-inflammatory cytokines.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000800702&lng=en&tlng=enPro-inflammatory cytokinesAnti-inflammatory cytokinesGlucose tolerance testInflammationPostprandial glycemia |
| spellingShingle | W. Eik Filho S.S. Marcon T. Krupek I.T.S. Previdelli O.C.N. Pereira M.A.R.C.P. Silva R.B. Bazotte Blood levels of pro-inflammatory and anti-inflammatory cytokines during an oral glucose tolerance test in patients with symptoms suggesting reactive hypoglycemia Brazilian Journal of Medical and Biological Research Pro-inflammatory cytokines Anti-inflammatory cytokines Glucose tolerance test Inflammation Postprandial glycemia |
| title | Blood levels of pro-inflammatory and anti-inflammatory cytokines during an oral glucose tolerance test in patients with symptoms suggesting reactive hypoglycemia |
| title_full | Blood levels of pro-inflammatory and anti-inflammatory cytokines during an oral glucose tolerance test in patients with symptoms suggesting reactive hypoglycemia |
| title_fullStr | Blood levels of pro-inflammatory and anti-inflammatory cytokines during an oral glucose tolerance test in patients with symptoms suggesting reactive hypoglycemia |
| title_full_unstemmed | Blood levels of pro-inflammatory and anti-inflammatory cytokines during an oral glucose tolerance test in patients with symptoms suggesting reactive hypoglycemia |
| title_short | Blood levels of pro-inflammatory and anti-inflammatory cytokines during an oral glucose tolerance test in patients with symptoms suggesting reactive hypoglycemia |
| title_sort | blood levels of pro inflammatory and anti inflammatory cytokines during an oral glucose tolerance test in patients with symptoms suggesting reactive hypoglycemia |
| topic | Pro-inflammatory cytokines Anti-inflammatory cytokines Glucose tolerance test Inflammation Postprandial glycemia |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000800702&lng=en&tlng=en |
| work_keys_str_mv | AT weikfilho bloodlevelsofproinflammatoryandantiinflammatorycytokinesduringanoralglucosetolerancetestinpatientswithsymptomssuggestingreactivehypoglycemia AT ssmarcon bloodlevelsofproinflammatoryandantiinflammatorycytokinesduringanoralglucosetolerancetestinpatientswithsymptomssuggestingreactivehypoglycemia AT tkrupek bloodlevelsofproinflammatoryandantiinflammatorycytokinesduringanoralglucosetolerancetestinpatientswithsymptomssuggestingreactivehypoglycemia AT itsprevidelli bloodlevelsofproinflammatoryandantiinflammatorycytokinesduringanoralglucosetolerancetestinpatientswithsymptomssuggestingreactivehypoglycemia AT ocnpereira bloodlevelsofproinflammatoryandantiinflammatorycytokinesduringanoralglucosetolerancetestinpatientswithsymptomssuggestingreactivehypoglycemia AT marcpsilva bloodlevelsofproinflammatoryandantiinflammatorycytokinesduringanoralglucosetolerancetestinpatientswithsymptomssuggestingreactivehypoglycemia AT rbbazotte bloodlevelsofproinflammatoryandantiinflammatorycytokinesduringanoralglucosetolerancetestinpatientswithsymptomssuggestingreactivehypoglycemia |