Induction of tetraploids in Paper Mulberry (Broussonetia papyrifera (L.) L’Hér. ex Vent.) by colchicine
Abstract Background Broussonetia papyrifera (L.) L’Hér. ex Vent. has the characteristics of strong stress resistance, high crude protein content, and pruning tolerance. It is an ecological, economic, and medicinal plant. Polyploid plants usually perform better than their corresponding diploid plants...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-11-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-023-04487-2 |
| _version_ | 1797577141177548800 |
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| author | Jiana Lin Bingnan Zhang Jintuo Zou Zhen Luo Hao Yang Peng Zhou Xiaoyang Chen Wei Zhou |
| author_facet | Jiana Lin Bingnan Zhang Jintuo Zou Zhen Luo Hao Yang Peng Zhou Xiaoyang Chen Wei Zhou |
| author_sort | Jiana Lin |
| collection | DOAJ |
| description | Abstract Background Broussonetia papyrifera (L.) L’Hér. ex Vent. has the characteristics of strong stress resistance, high crude protein content, and pruning tolerance. It is an ecological, economic, and medicinal plant. Polyploid plants usually perform better than their corresponding diploid plants in terms of nutrients, active substances, and stress resistance. Results In this study, the leaves, calli, and seeds of diploid B. papyrifera were used for tetraploid induction by colchicine. The induction effect of colchicine on B. papyrifera was summarized through the early morphology, chromosome count and flow cytometry. It was concluded that the best induction effect (18.6%) was obtained when the leaves of B. papyrifera were treated in liquid MS (Murashige and Skoog) medium containing 450 mg·L-1 colchicine for 3 d. The comparative analysis of the growth characteristics of diploid and tetraploid B. papyrifera showed that tetraploid B. papyrifera has larger ground diameter, larger stomata, thicker palisade tissue and thicker sponge tissue than diploid B. papyrifera. In addition, the measurement of photosynthetic features also showed that tetraploids had higher chlorophyll content and higher photosynthetic rates. Conclusion This study showed that tetraploid B. papyrifera could be obtained by treating leaves, callus and seeds with liquid and solid colchicine, but the induction efficiency was different. Moreover, there were differences in stomata, leaf cell structure and photosynthetic features between tetraploid B. papyrifera and its corresponding diploid. The induced tetraploid B. papyrifera can provide a technical basis and breeding material for the creation of B. papyrifera germplasm resources in the future. |
| first_indexed | 2024-03-10T22:03:48Z |
| format | Article |
| id | doaj.art-e98fab9433f243f08b3110a524e0f0f4 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-03-10T22:03:48Z |
| publishDate | 2023-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-e98fab9433f243f08b3110a524e0f0f42023-11-19T12:52:35ZengBMCBMC Plant Biology1471-22292023-11-0123111310.1186/s12870-023-04487-2Induction of tetraploids in Paper Mulberry (Broussonetia papyrifera (L.) L’Hér. ex Vent.) by colchicineJiana Lin0Bingnan Zhang1Jintuo Zou2Zhen Luo3Hao Yang4Peng Zhou5Xiaoyang Chen6Wei Zhou7State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources (South China Agricultural University)State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources (South China Agricultural University)Guangdong Engineering Technology Research Center of Agricultural and Forestry Biomass, South China Agricultural UniversityCollege of Forestry and Landscape Architecture, South China Agricultural UniversityCollege of Forestry and Landscape Architecture, South China Agricultural UniversityGuangdong Eco-Engineering PolytechnicState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources (South China Agricultural University)Guangdong Province Research Center of Woody Forage Engineering TechnologyAbstract Background Broussonetia papyrifera (L.) L’Hér. ex Vent. has the characteristics of strong stress resistance, high crude protein content, and pruning tolerance. It is an ecological, economic, and medicinal plant. Polyploid plants usually perform better than their corresponding diploid plants in terms of nutrients, active substances, and stress resistance. Results In this study, the leaves, calli, and seeds of diploid B. papyrifera were used for tetraploid induction by colchicine. The induction effect of colchicine on B. papyrifera was summarized through the early morphology, chromosome count and flow cytometry. It was concluded that the best induction effect (18.6%) was obtained when the leaves of B. papyrifera were treated in liquid MS (Murashige and Skoog) medium containing 450 mg·L-1 colchicine for 3 d. The comparative analysis of the growth characteristics of diploid and tetraploid B. papyrifera showed that tetraploid B. papyrifera has larger ground diameter, larger stomata, thicker palisade tissue and thicker sponge tissue than diploid B. papyrifera. In addition, the measurement of photosynthetic features also showed that tetraploids had higher chlorophyll content and higher photosynthetic rates. Conclusion This study showed that tetraploid B. papyrifera could be obtained by treating leaves, callus and seeds with liquid and solid colchicine, but the induction efficiency was different. Moreover, there were differences in stomata, leaf cell structure and photosynthetic features between tetraploid B. papyrifera and its corresponding diploid. The induced tetraploid B. papyrifera can provide a technical basis and breeding material for the creation of B. papyrifera germplasm resources in the future.https://doi.org/10.1186/s12870-023-04487-2Broussonetia papyriferaPolyploidColchicinePhotosynthetic features |
| spellingShingle | Jiana Lin Bingnan Zhang Jintuo Zou Zhen Luo Hao Yang Peng Zhou Xiaoyang Chen Wei Zhou Induction of tetraploids in Paper Mulberry (Broussonetia papyrifera (L.) L’Hér. ex Vent.) by colchicine BMC Plant Biology Broussonetia papyrifera Polyploid Colchicine Photosynthetic features |
| title | Induction of tetraploids in Paper Mulberry (Broussonetia papyrifera (L.) L’Hér. ex Vent.) by colchicine |
| title_full | Induction of tetraploids in Paper Mulberry (Broussonetia papyrifera (L.) L’Hér. ex Vent.) by colchicine |
| title_fullStr | Induction of tetraploids in Paper Mulberry (Broussonetia papyrifera (L.) L’Hér. ex Vent.) by colchicine |
| title_full_unstemmed | Induction of tetraploids in Paper Mulberry (Broussonetia papyrifera (L.) L’Hér. ex Vent.) by colchicine |
| title_short | Induction of tetraploids in Paper Mulberry (Broussonetia papyrifera (L.) L’Hér. ex Vent.) by colchicine |
| title_sort | induction of tetraploids in paper mulberry broussonetia papyrifera l l her ex vent by colchicine |
| topic | Broussonetia papyrifera Polyploid Colchicine Photosynthetic features |
| url | https://doi.org/10.1186/s12870-023-04487-2 |
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