Association of CYP19A1 gene polymorphisms with reproductive traits in pigs
Porcine reproductive traits are characterized by low heritability, making improvement by traditional selective breeding rather difficult. Molecular breeding offers powerful approaches to overcome previous limitations and is expected to generate economic benefits via progress in pig breeding. Cytochr...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2017-07-01
|
| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311916615204 |
| _version_ | 1818739120841687040 |
|---|---|
| author | Rong ZHOU Ya-lan YANG Ying LIU Qi-mei CHEN Jie CHEN Kui LI |
| author_facet | Rong ZHOU Ya-lan YANG Ying LIU Qi-mei CHEN Jie CHEN Kui LI |
| author_sort | Rong ZHOU |
| collection | DOAJ |
| description | Porcine reproductive traits are characterized by low heritability, making improvement by traditional selective breeding rather difficult. Molecular breeding offers powerful approaches to overcome previous limitations and is expected to generate economic benefits via progress in pig breeding. Cytochrome P450 family 19 subfamily A polypeptide 1 (CYP19A1) gene is a key enzyme of estradiol biosynthesis that plays an important role in the establishment of gestation and maintenance of pregnancy. In this study, the sequence and structure characteristics of the porcine CYP19A1 gene was analyzed and expression patterns of CYP19A1 in different tissues of adult female pigs were detected. Fourteen single-nucleotide polymorphisms (SNPs) in the exons and introns of porcine CYP19A1 were identified and genotyped using the Sequenom MassARRAY platform, after which the allele frequency of each SNP was analyzed. The association between CYP19A1 SNPs and litter size and piglet birth weight was assessed in a crossbred pig population (n=375). The expression pattern of CYP19A1 revealed that it was highly expressed in the ovary, spleen, and uterus and lowly expressed in the other tissues. Moreover, one SNP, rs341891833, was significantly associated with piglet birth weight during the multiparity period (P<0.01). We concluded that CYP19A1 could be used as a candidate molecular marker in breeding aimed at rapid improvement of the reproductive characteristics of pigs. |
| first_indexed | 2024-12-18T01:19:47Z |
| format | Article |
| id | doaj.art-e994077218cf447a885b9156982b1b53 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-18T01:19:47Z |
| publishDate | 2017-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-e994077218cf447a885b9156982b1b532022-12-21T21:25:52ZengElsevierJournal of Integrative Agriculture2095-31192017-07-0116715581565Association of CYP19A1 gene polymorphisms with reproductive traits in pigsRong ZHOU0Ya-lan YANG1Ying LIU2Qi-mei CHEN3Jie CHEN4Kui LI5Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, P.R.China; Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation, Ministry of Agriculture/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaKey Laboratory of Farm Animal Genetic Resources and Germplasm Innovation, Ministry of Agriculture/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaKey Laboratory of Farm Animal Genetic Resources and Germplasm Innovation, Ministry of Agriculture/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaShandong Lansi Seeds Industry Co., Ltd., Rizhao 276800, P.R.ChinaDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, P.R.China; Correspondence CHEN Jie, Tel/Fax: +86-25-84396347Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, P.R.China; Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation, Ministry of Agriculture/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China; LI Kui, Tel/Fax: +86-10-62813822Porcine reproductive traits are characterized by low heritability, making improvement by traditional selective breeding rather difficult. Molecular breeding offers powerful approaches to overcome previous limitations and is expected to generate economic benefits via progress in pig breeding. Cytochrome P450 family 19 subfamily A polypeptide 1 (CYP19A1) gene is a key enzyme of estradiol biosynthesis that plays an important role in the establishment of gestation and maintenance of pregnancy. In this study, the sequence and structure characteristics of the porcine CYP19A1 gene was analyzed and expression patterns of CYP19A1 in different tissues of adult female pigs were detected. Fourteen single-nucleotide polymorphisms (SNPs) in the exons and introns of porcine CYP19A1 were identified and genotyped using the Sequenom MassARRAY platform, after which the allele frequency of each SNP was analyzed. The association between CYP19A1 SNPs and litter size and piglet birth weight was assessed in a crossbred pig population (n=375). The expression pattern of CYP19A1 revealed that it was highly expressed in the ovary, spleen, and uterus and lowly expressed in the other tissues. Moreover, one SNP, rs341891833, was significantly associated with piglet birth weight during the multiparity period (P<0.01). We concluded that CYP19A1 could be used as a candidate molecular marker in breeding aimed at rapid improvement of the reproductive characteristics of pigs.http://www.sciencedirect.com/science/article/pii/S2095311916615204CYP19A1pigreproductionbirth weightsingle nucleotide polymorphism |
| spellingShingle | Rong ZHOU Ya-lan YANG Ying LIU Qi-mei CHEN Jie CHEN Kui LI Association of CYP19A1 gene polymorphisms with reproductive traits in pigs Journal of Integrative Agriculture CYP19A1 pig reproduction birth weight single nucleotide polymorphism |
| title | Association of CYP19A1 gene polymorphisms with reproductive traits in pigs |
| title_full | Association of CYP19A1 gene polymorphisms with reproductive traits in pigs |
| title_fullStr | Association of CYP19A1 gene polymorphisms with reproductive traits in pigs |
| title_full_unstemmed | Association of CYP19A1 gene polymorphisms with reproductive traits in pigs |
| title_short | Association of CYP19A1 gene polymorphisms with reproductive traits in pigs |
| title_sort | association of cyp19a1 gene polymorphisms with reproductive traits in pigs |
| topic | CYP19A1 pig reproduction birth weight single nucleotide polymorphism |
| url | http://www.sciencedirect.com/science/article/pii/S2095311916615204 |
| work_keys_str_mv | AT rongzhou associationofcyp19a1genepolymorphismswithreproductivetraitsinpigs AT yalanyang associationofcyp19a1genepolymorphismswithreproductivetraitsinpigs AT yingliu associationofcyp19a1genepolymorphismswithreproductivetraitsinpigs AT qimeichen associationofcyp19a1genepolymorphismswithreproductivetraitsinpigs AT jiechen associationofcyp19a1genepolymorphismswithreproductivetraitsinpigs AT kuili associationofcyp19a1genepolymorphismswithreproductivetraitsinpigs |