Investigation of crystalline lens overshooting: ex vivo experiment and optomechanical simulation results
Introduction: Crystalline lens overshooting refers to a situation in which the lens momentarily shifts too much from its typical location immediately after stopping the rotational movement of the eye globe. This movement can be observed using an optical technique called Purkinje imaging.Methods: In...
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| Format: | Article |
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Frontiers Media S.A.
2024-04-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1348774/full |
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| author | Ali Dahaghin Milad Salimibani Agnieszka Boszczyk Agnieszka Jóźwik Marta Skrok Jorge Grasa Jorge Grasa Damian Siedlecki |
| author_facet | Ali Dahaghin Milad Salimibani Agnieszka Boszczyk Agnieszka Jóźwik Marta Skrok Jorge Grasa Jorge Grasa Damian Siedlecki |
| author_sort | Ali Dahaghin |
| collection | DOAJ |
| description | Introduction: Crystalline lens overshooting refers to a situation in which the lens momentarily shifts too much from its typical location immediately after stopping the rotational movement of the eye globe. This movement can be observed using an optical technique called Purkinje imaging.Methods: In this work, an experimental setup was designed to reproduce this effect ex vivo using a fresh porcine eye. The sample was rotated 90° around its centroid using a high-velocity rotation stage, and the Purkinje image sequences were recorded, allowing us to quantify the overshooting effect. The numerical part of the study consisted of developing a computational model of the eye, based on the finite element method, that allowed us to understand the biomechanical behavior of the different tissues in this dynamic scenario. A 2D fluid–structure interaction model of the porcine eye globe, considering both the solid parts and humors, was created to reproduce the experimental outcomes.Results: Outputs of the simulation were analyzed using an optical simulation software package to assess whether the mechanical model behaves optically like the real ex vivo eye. The simulation predicted the experimental results by carefully adjusting the mechanical properties of the zonular fibers and the damping factor.Conclusion: This study effectively demonstrates the importance of characterizing the dynamic mechanical properties of the eye tissues to properly comprehend and predict the overshooting effect. |
| first_indexed | 2024-04-24T11:56:00Z |
| format | Article |
| id | doaj.art-e9949bf67277437bb742563c21f1db8d |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-24T11:56:00Z |
| publishDate | 2024-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-e9949bf67277437bb742563c21f1db8d2024-04-09T05:00:58ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852024-04-011210.3389/fbioe.2024.13487741348774Investigation of crystalline lens overshooting: ex vivo experiment and optomechanical simulation resultsAli Dahaghin0Milad Salimibani1Agnieszka Boszczyk2Agnieszka Jóźwik3Marta Skrok4Jorge Grasa5Jorge Grasa6Damian Siedlecki7Department of Optics and Photonics, Wroclaw University of Science and Technology, Wrocław, PolandDepartment of Optics and Photonics, Wroclaw University of Science and Technology, Wrocław, PolandDepartment of Optics and Photonics, Wroclaw University of Science and Technology, Wrocław, PolandDepartment of Optics and Photonics, Wroclaw University of Science and Technology, Wrocław, PolandDepartment of Optics and Photonics, Wroclaw University of Science and Technology, Wrocław, PolandAragón Institute of Engineering Research (I3A), University of Zaragoza, Zaragoza, SpainCentro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Zaragoza, SpainDepartment of Optics and Photonics, Wroclaw University of Science and Technology, Wrocław, PolandIntroduction: Crystalline lens overshooting refers to a situation in which the lens momentarily shifts too much from its typical location immediately after stopping the rotational movement of the eye globe. This movement can be observed using an optical technique called Purkinje imaging.Methods: In this work, an experimental setup was designed to reproduce this effect ex vivo using a fresh porcine eye. The sample was rotated 90° around its centroid using a high-velocity rotation stage, and the Purkinje image sequences were recorded, allowing us to quantify the overshooting effect. The numerical part of the study consisted of developing a computational model of the eye, based on the finite element method, that allowed us to understand the biomechanical behavior of the different tissues in this dynamic scenario. A 2D fluid–structure interaction model of the porcine eye globe, considering both the solid parts and humors, was created to reproduce the experimental outcomes.Results: Outputs of the simulation were analyzed using an optical simulation software package to assess whether the mechanical model behaves optically like the real ex vivo eye. The simulation predicted the experimental results by carefully adjusting the mechanical properties of the zonular fibers and the damping factor.Conclusion: This study effectively demonstrates the importance of characterizing the dynamic mechanical properties of the eye tissues to properly comprehend and predict the overshooting effect.https://www.frontiersin.org/articles/10.3389/fbioe.2024.1348774/fullporcine crystalline lensinertial motionwobblingovershootingfinite-element methodfluid–structure interaction |
| spellingShingle | Ali Dahaghin Milad Salimibani Agnieszka Boszczyk Agnieszka Jóźwik Marta Skrok Jorge Grasa Jorge Grasa Damian Siedlecki Investigation of crystalline lens overshooting: ex vivo experiment and optomechanical simulation results Frontiers in Bioengineering and Biotechnology porcine crystalline lens inertial motion wobbling overshooting finite-element method fluid–structure interaction |
| title | Investigation of crystalline lens overshooting: ex vivo experiment and optomechanical simulation results |
| title_full | Investigation of crystalline lens overshooting: ex vivo experiment and optomechanical simulation results |
| title_fullStr | Investigation of crystalline lens overshooting: ex vivo experiment and optomechanical simulation results |
| title_full_unstemmed | Investigation of crystalline lens overshooting: ex vivo experiment and optomechanical simulation results |
| title_short | Investigation of crystalline lens overshooting: ex vivo experiment and optomechanical simulation results |
| title_sort | investigation of crystalline lens overshooting ex vivo experiment and optomechanical simulation results |
| topic | porcine crystalline lens inertial motion wobbling overshooting finite-element method fluid–structure interaction |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1348774/full |
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