Protein Hydrolysates from Anchovy (<i>Engraulis encrasicolus)</i> Waste: In Vitro and In Vivo Biological Activities
Fish waste utilization to obtain protein hydrolysates has been demonstrated to be a useful strategy to face both environmental and economic impacts while obtaining high-value products with remarkable biological and nutritional properties. In the present study, protein hydrolysates obtained from anch...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Formato: | Artigo |
| Idioma: | English |
| Publicado em: |
MDPI AG
2020-01-01
|
| Colecção: | Marine Drugs |
| Assuntos: | |
| Acesso em linha: | https://www.mdpi.com/1660-3397/18/2/86 |
| _version_ | 1828370005682552832 |
|---|---|
| author | Alessia Giannetto Emanuela Esposito Marika Lanza Sabrina Oliva Kristian Riolo Simona Di Pietro Jessica Maria Abbate Giovanni Briguglio Giovanni Cassata Luca Cicero Francesco Macrì |
| author_facet | Alessia Giannetto Emanuela Esposito Marika Lanza Sabrina Oliva Kristian Riolo Simona Di Pietro Jessica Maria Abbate Giovanni Briguglio Giovanni Cassata Luca Cicero Francesco Macrì |
| author_sort | Alessia Giannetto |
| collection | DOAJ |
| description | Fish waste utilization to obtain protein hydrolysates has been demonstrated to be a useful strategy to face both environmental and economic impacts while obtaining high-value products with remarkable biological and nutritional properties. In the present study, protein hydrolysates obtained from anchovy <i>Engraulis encrasicolus</i> (APH) by-products were assessed for their potential biological activities in both in vitro and in vivo models. The treatment with APH exerted a significant protection against LPS-induced inflammation in RAW 264.7 cells, decreasing the protein expression of pro-inflammatory mediators (i.e., COX-2) and inhibiting the nuclear translocation of NF-κB through IκB-α. Moreover, APH modulated the expression of iNOS, MnSOD and HO-1, thus decreasing the severity of oxidative stress. The supplementation of APH in the diet of ApoE knockout mice down-regulated the proinflammatory cytokines (i.e., <i>TNF-α</i>, <i>IL-1α</i>, <i>IL-1β</i>, <i>IL-6</i>) in both aorta and heart tissues, and modulated the expression of oxidative stress-related genes (<i>Cu/ZnSod</i>, <i>MnSod</i>, <i>Cat</i>, <i>Gpx</i> and <i>Ho</i>), indicating that APH can exert a beneficial role, having anti-inflammatory and antioxidant activities. The nutritional properties of APH, together with their biological activities herein reported, highlight the possibility of obtaining bioactive molecules from fish waste and encourage their use as potential nutraceuticals in food and pharmaceutical industries in the next future. |
| first_indexed | 2024-04-14T06:32:09Z |
| format | Article |
| id | doaj.art-e995c94a2fbb4f3b94e1b056d2575ba4 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-14T06:32:09Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-e995c94a2fbb4f3b94e1b056d2575ba42022-12-22T02:07:36ZengMDPI AGMarine Drugs1660-33972020-01-011828610.3390/md18020086md18020086Protein Hydrolysates from Anchovy (<i>Engraulis encrasicolus)</i> Waste: In Vitro and In Vivo Biological ActivitiesAlessia Giannetto0Emanuela Esposito1Marika Lanza2Sabrina Oliva3Kristian Riolo4Simona Di Pietro5Jessica Maria Abbate6Giovanni Briguglio7Giovanni Cassata8Luca Cicero9Francesco Macrì10Department of Chemical, Biological, Pharmaceutical and Environmental Sciences. University of Messina, Viale Ferdinando Stagno d’Alcontres, 31, 98166 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences. University of Messina, Viale Ferdinando Stagno d’Alcontres, 31, 98166 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences. University of Messina, Viale Ferdinando Stagno d’Alcontres, 31, 98166 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences. University of Messina, Viale Ferdinando Stagno d’Alcontres, 31, 98166 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences. University of Messina, Viale Ferdinando Stagno d’Alcontres, 31, 98166 Messina, ItalyDepartment of Veterinary Sciences, University of Messina, Polo Universitario SS. Annunziata, 98168 Messina, ItalyVeterinary Practitian, 98167 Messina, ItalyVeterinary Practitian, 98167 Messina, ItalyZooprophylactic Institute of Sicily “A. Mirri”, 90129 Palermo, ItalyZooprophylactic Institute of Sicily “A. Mirri”, 90129 Palermo, ItalyDepartment of Veterinary Sciences, University of Messina, Polo Universitario SS. Annunziata, 98168 Messina, ItalyFish waste utilization to obtain protein hydrolysates has been demonstrated to be a useful strategy to face both environmental and economic impacts while obtaining high-value products with remarkable biological and nutritional properties. In the present study, protein hydrolysates obtained from anchovy <i>Engraulis encrasicolus</i> (APH) by-products were assessed for their potential biological activities in both in vitro and in vivo models. The treatment with APH exerted a significant protection against LPS-induced inflammation in RAW 264.7 cells, decreasing the protein expression of pro-inflammatory mediators (i.e., COX-2) and inhibiting the nuclear translocation of NF-κB through IκB-α. Moreover, APH modulated the expression of iNOS, MnSOD and HO-1, thus decreasing the severity of oxidative stress. The supplementation of APH in the diet of ApoE knockout mice down-regulated the proinflammatory cytokines (i.e., <i>TNF-α</i>, <i>IL-1α</i>, <i>IL-1β</i>, <i>IL-6</i>) in both aorta and heart tissues, and modulated the expression of oxidative stress-related genes (<i>Cu/ZnSod</i>, <i>MnSod</i>, <i>Cat</i>, <i>Gpx</i> and <i>Ho</i>), indicating that APH can exert a beneficial role, having anti-inflammatory and antioxidant activities. The nutritional properties of APH, together with their biological activities herein reported, highlight the possibility of obtaining bioactive molecules from fish waste and encourage their use as potential nutraceuticals in food and pharmaceutical industries in the next future.https://www.mdpi.com/1660-3397/18/2/86marine organismsanchovy <i>engraulis encrasicolus</i>bioactive peptidesantioxidantanti-inflammatory |
| spellingShingle | Alessia Giannetto Emanuela Esposito Marika Lanza Sabrina Oliva Kristian Riolo Simona Di Pietro Jessica Maria Abbate Giovanni Briguglio Giovanni Cassata Luca Cicero Francesco Macrì Protein Hydrolysates from Anchovy (<i>Engraulis encrasicolus)</i> Waste: In Vitro and In Vivo Biological Activities Marine Drugs marine organisms anchovy <i>engraulis encrasicolus</i> bioactive peptides antioxidant anti-inflammatory |
| title | Protein Hydrolysates from Anchovy (<i>Engraulis encrasicolus)</i> Waste: In Vitro and In Vivo Biological Activities |
| title_full | Protein Hydrolysates from Anchovy (<i>Engraulis encrasicolus)</i> Waste: In Vitro and In Vivo Biological Activities |
| title_fullStr | Protein Hydrolysates from Anchovy (<i>Engraulis encrasicolus)</i> Waste: In Vitro and In Vivo Biological Activities |
| title_full_unstemmed | Protein Hydrolysates from Anchovy (<i>Engraulis encrasicolus)</i> Waste: In Vitro and In Vivo Biological Activities |
| title_short | Protein Hydrolysates from Anchovy (<i>Engraulis encrasicolus)</i> Waste: In Vitro and In Vivo Biological Activities |
| title_sort | protein hydrolysates from anchovy i engraulis encrasicolus i waste in vitro and in vivo biological activities |
| topic | marine organisms anchovy <i>engraulis encrasicolus</i> bioactive peptides antioxidant anti-inflammatory |
| url | https://www.mdpi.com/1660-3397/18/2/86 |
| work_keys_str_mv | AT alessiagiannetto proteinhydrolysatesfromanchovyiengraulisencrasicolusiwasteinvitroandinvivobiologicalactivities AT emanuelaesposito proteinhydrolysatesfromanchovyiengraulisencrasicolusiwasteinvitroandinvivobiologicalactivities AT marikalanza proteinhydrolysatesfromanchovyiengraulisencrasicolusiwasteinvitroandinvivobiologicalactivities AT sabrinaoliva proteinhydrolysatesfromanchovyiengraulisencrasicolusiwasteinvitroandinvivobiologicalactivities AT kristianriolo proteinhydrolysatesfromanchovyiengraulisencrasicolusiwasteinvitroandinvivobiologicalactivities AT simonadipietro proteinhydrolysatesfromanchovyiengraulisencrasicolusiwasteinvitroandinvivobiologicalactivities AT jessicamariaabbate proteinhydrolysatesfromanchovyiengraulisencrasicolusiwasteinvitroandinvivobiologicalactivities AT giovannibriguglio proteinhydrolysatesfromanchovyiengraulisencrasicolusiwasteinvitroandinvivobiologicalactivities AT giovannicassata proteinhydrolysatesfromanchovyiengraulisencrasicolusiwasteinvitroandinvivobiologicalactivities AT lucacicero proteinhydrolysatesfromanchovyiengraulisencrasicolusiwasteinvitroandinvivobiologicalactivities AT francescomacri proteinhydrolysatesfromanchovyiengraulisencrasicolusiwasteinvitroandinvivobiologicalactivities |