A Functional Assay for the Determination of Heparin-Induced Thrombocytopenia via Flow Cytometry
Heparin-induced thrombocytopenia (HIT) is a life-threatening complication of heparin therapy (both unfractionated heparin and low-molecular-weight heparin). In our study, we examined a group of 122 patients with suspected HIT. The samples of all patients were analyzed in the first step using an immu...
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MDPI AG
2023-09-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/13/18/3019 |
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| author | Ingrid Skornova Tomas Simurda Lucia Stanciakova Viliam Lauko Pavol Holly Matej Samos Tomas Bolek Martin Schnierer Miroslava Drotarova Kristina Maria Belakova Juraj Sokol Jan Stasko Marian Mokan Jaroslav Gumulec Leona Chrastinova |
| author_facet | Ingrid Skornova Tomas Simurda Lucia Stanciakova Viliam Lauko Pavol Holly Matej Samos Tomas Bolek Martin Schnierer Miroslava Drotarova Kristina Maria Belakova Juraj Sokol Jan Stasko Marian Mokan Jaroslav Gumulec Leona Chrastinova |
| author_sort | Ingrid Skornova |
| collection | DOAJ |
| description | Heparin-induced thrombocytopenia (HIT) is a life-threatening complication of heparin therapy (both unfractionated heparin and low-molecular-weight heparin). In our study, we examined a group of 122 patients with suspected HIT. The samples of all patients were analyzed in the first step using an immunoassay (ID-PaGIA Heparin/PF4, Hemos1L-Acustar HIT IgG, ZYMUTEST HIA Monostrip IgG) to detect the presence of antibodies against heparin–PF4 complexes (platelet factor 4). When the immunoassay was positive, the sample was subsequently analyzed for HIT with a functional flow cytometry assay, the HITAlert kit, the purpose of which was to demonstrate the ability of the antibodies present to activate platelets. A diagnosis of HIT can be made only after a positive functional test result. In this article, we present an overview of our practical experience with the use of the new functional method of analysis, HIT, with flow cytometry. In this work, we compared the mutual sensitivity of two functional tests, SRA and the flow cytometry HITAlert kit, in patients perceived as being at risk for HIT. This work aims to delineate the principle, procedure, advantages, pitfalls, and possibilities of the application of the functional test HITAlert using flow cytometry. |
| first_indexed | 2024-03-10T22:52:45Z |
| format | Article |
| id | doaj.art-e998bf12e7e04cfa945152c1b65dbb09 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-10T22:52:45Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-e998bf12e7e04cfa945152c1b65dbb092023-11-19T10:14:46ZengMDPI AGDiagnostics2075-44182023-09-011318301910.3390/diagnostics13183019A Functional Assay for the Determination of Heparin-Induced Thrombocytopenia via Flow CytometryIngrid Skornova0Tomas Simurda1Lucia Stanciakova2Viliam Lauko3Pavol Holly4Matej Samos5Tomas Bolek6Martin Schnierer7Miroslava Drotarova8Kristina Maria Belakova9Juraj Sokol10Jan Stasko11Marian Mokan12Jaroslav Gumulec13Leona Chrastinova14National Center of Hemostasis and Thrombosis, Department of Hematology and Transfusiology, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin and University Hospital in Martin, 03601 Martin, SlovakiaNational Center of Hemostasis and Thrombosis, Department of Hematology and Transfusiology, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin and University Hospital in Martin, 03601 Martin, SlovakiaNational Center of Hemostasis and Thrombosis, Department of Hematology and Transfusiology, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin and University Hospital in Martin, 03601 Martin, SlovakiaNational Institute of Cardiovascular Diseases, 83348 Bratislava, SlovakiaNational Center of Hemostasis and Thrombosis, Department of Hematology and Transfusiology, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin and University Hospital in Martin, 03601 Martin, SlovakiaDepartment of Internal Medicine I, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, 03601 Martin, SlovakiaDepartment of Internal Medicine I, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, 03601 Martin, SlovakiaDepartment of Gastroenterology Medicine, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, 03601 Martin, SlovakiaNational Center of Hemostasis and Thrombosis, Department of Hematology and Transfusiology, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin and University Hospital in Martin, 03601 Martin, SlovakiaNational Center of Hemostasis and Thrombosis, Department of Hematology and Transfusiology, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin and University Hospital in Martin, 03601 Martin, SlovakiaNational Center of Hemostasis and Thrombosis, Department of Hematology and Transfusiology, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin and University Hospital in Martin, 03601 Martin, SlovakiaNational Center of Hemostasis and Thrombosis, Department of Hematology and Transfusiology, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin and University Hospital in Martin, 03601 Martin, SlovakiaDepartment of Internal Medicine I, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, 03601 Martin, SlovakiaClinic of Hemato-Oncology, Faculty Hospital in Ostrava, 708 00 Ostrava, Czech RepublicInstitute of Hematology and Blood Transfusion in Prague, 128 20 Nove Mesto, Czech RepublicHeparin-induced thrombocytopenia (HIT) is a life-threatening complication of heparin therapy (both unfractionated heparin and low-molecular-weight heparin). In our study, we examined a group of 122 patients with suspected HIT. The samples of all patients were analyzed in the first step using an immunoassay (ID-PaGIA Heparin/PF4, Hemos1L-Acustar HIT IgG, ZYMUTEST HIA Monostrip IgG) to detect the presence of antibodies against heparin–PF4 complexes (platelet factor 4). When the immunoassay was positive, the sample was subsequently analyzed for HIT with a functional flow cytometry assay, the HITAlert kit, the purpose of which was to demonstrate the ability of the antibodies present to activate platelets. A diagnosis of HIT can be made only after a positive functional test result. In this article, we present an overview of our practical experience with the use of the new functional method of analysis, HIT, with flow cytometry. In this work, we compared the mutual sensitivity of two functional tests, SRA and the flow cytometry HITAlert kit, in patients perceived as being at risk for HIT. This work aims to delineate the principle, procedure, advantages, pitfalls, and possibilities of the application of the functional test HITAlert using flow cytometry.https://www.mdpi.com/2075-4418/13/18/3019heparin-induced thrombocytopenia (HIT)heparin–PF4 complex (platelet factor 4)immunological and functional assaysserotonin release assay (SRA) |
| spellingShingle | Ingrid Skornova Tomas Simurda Lucia Stanciakova Viliam Lauko Pavol Holly Matej Samos Tomas Bolek Martin Schnierer Miroslava Drotarova Kristina Maria Belakova Juraj Sokol Jan Stasko Marian Mokan Jaroslav Gumulec Leona Chrastinova A Functional Assay for the Determination of Heparin-Induced Thrombocytopenia via Flow Cytometry Diagnostics heparin-induced thrombocytopenia (HIT) heparin–PF4 complex (platelet factor 4) immunological and functional assays serotonin release assay (SRA) |
| title | A Functional Assay for the Determination of Heparin-Induced Thrombocytopenia via Flow Cytometry |
| title_full | A Functional Assay for the Determination of Heparin-Induced Thrombocytopenia via Flow Cytometry |
| title_fullStr | A Functional Assay for the Determination of Heparin-Induced Thrombocytopenia via Flow Cytometry |
| title_full_unstemmed | A Functional Assay for the Determination of Heparin-Induced Thrombocytopenia via Flow Cytometry |
| title_short | A Functional Assay for the Determination of Heparin-Induced Thrombocytopenia via Flow Cytometry |
| title_sort | functional assay for the determination of heparin induced thrombocytopenia via flow cytometry |
| topic | heparin-induced thrombocytopenia (HIT) heparin–PF4 complex (platelet factor 4) immunological and functional assays serotonin release assay (SRA) |
| url | https://www.mdpi.com/2075-4418/13/18/3019 |
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