Use of response surface method for maximizing the production of arginine deiminase by Pseudomonas putida
Statistically designed experiments were used to optimize the production of arginine deiminase (ADI) by Pseudomonas putida KT2440 in batch culture. A Plackett-Burman design involving eleven factors showed that ADI production was most influenced by the initial pH and the initial concentrations of gluc...
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| Format: | Article |
| Language: | English |
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Elsevier
2016-06-01
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| Series: | Biotechnology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X16300091 |
| _version_ | 1818683997755015168 |
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| author | Mahesh D. Patil Kiran D. Shinde Gopal Patel Yusuf Chisti Uttam Chand Banerjee |
| author_facet | Mahesh D. Patil Kiran D. Shinde Gopal Patel Yusuf Chisti Uttam Chand Banerjee |
| author_sort | Mahesh D. Patil |
| collection | DOAJ |
| description | Statistically designed experiments were used to optimize the production of arginine deiminase (ADI) by Pseudomonas putida KT2440 in batch culture. A Plackett-Burman design involving eleven factors showed that ADI production was most influenced by the initial pH and the initial concentrations of glucose and yeast extract. A central composite experimental design showed that the optimal values of these factors were 8.0, 10 g/L and 12.5 g/L, respectively. The other components of the optimal culture medium were bacto peptone 7.5 g/L, Triton X–100 0.30% (v/v), and arginine 3 g/L, for a culture temperature of 25 °C. Compared with the basal medium, the ADI activity in the optimized medium had nearly 4.5-fold increase (4.31 U/mL). The optimized medium was then used for a further study of ADI production in a 14 L stirred tank bioreactor. The agitation speed and the aeration rates were varied to determine suitable values of these variables. |
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| format | Article |
| id | doaj.art-e999d48efd3f452492b5aa0e7f65fb97 |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-12-17T10:43:38Z |
| publishDate | 2016-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-e999d48efd3f452492b5aa0e7f65fb972022-12-21T21:52:12ZengElsevierBiotechnology Reports2215-017X2016-06-0110C293710.1016/j.btre.2016.03.002Use of response surface method for maximizing the production of arginine deiminase by Pseudomonas putidaMahesh D. Patil0Kiran D. Shinde1Gopal Patel2Yusuf Chisti3Uttam Chand Banerjee4Department of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S. Nagar, 160062 Punjab, IndiaDepartment of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S. Nagar, 160062 Punjab, IndiaDepartment of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S. Nagar, 160062 Punjab, IndiaSchool of Engineering, Massey University, Private Bag 11 222, Palmerston North, New ZealandDepartment of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S. Nagar, 160062 Punjab, IndiaStatistically designed experiments were used to optimize the production of arginine deiminase (ADI) by Pseudomonas putida KT2440 in batch culture. A Plackett-Burman design involving eleven factors showed that ADI production was most influenced by the initial pH and the initial concentrations of glucose and yeast extract. A central composite experimental design showed that the optimal values of these factors were 8.0, 10 g/L and 12.5 g/L, respectively. The other components of the optimal culture medium were bacto peptone 7.5 g/L, Triton X–100 0.30% (v/v), and arginine 3 g/L, for a culture temperature of 25 °C. Compared with the basal medium, the ADI activity in the optimized medium had nearly 4.5-fold increase (4.31 U/mL). The optimized medium was then used for a further study of ADI production in a 14 L stirred tank bioreactor. The agitation speed and the aeration rates were varied to determine suitable values of these variables.http://www.sciencedirect.com/science/article/pii/S2215017X16300091Arginine deiminaseEnzyme productionFermentationEnzyme bioreactorsPseudomonas putida KT2440Submerged culture |
| spellingShingle | Mahesh D. Patil Kiran D. Shinde Gopal Patel Yusuf Chisti Uttam Chand Banerjee Use of response surface method for maximizing the production of arginine deiminase by Pseudomonas putida Biotechnology Reports Arginine deiminase Enzyme production Fermentation Enzyme bioreactors Pseudomonas putida KT2440 Submerged culture |
| title | Use of response surface method for maximizing the production of arginine deiminase by Pseudomonas putida |
| title_full | Use of response surface method for maximizing the production of arginine deiminase by Pseudomonas putida |
| title_fullStr | Use of response surface method for maximizing the production of arginine deiminase by Pseudomonas putida |
| title_full_unstemmed | Use of response surface method for maximizing the production of arginine deiminase by Pseudomonas putida |
| title_short | Use of response surface method for maximizing the production of arginine deiminase by Pseudomonas putida |
| title_sort | use of response surface method for maximizing the production of arginine deiminase by pseudomonas putida |
| topic | Arginine deiminase Enzyme production Fermentation Enzyme bioreactors Pseudomonas putida KT2440 Submerged culture |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X16300091 |
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