Megasporogenesis and Megagametogenesis in <i>Coelogyne speciosa</i> subsp. <i>fimbriata</i> (J.J.Sm.) Gravendeel (Orchidaceae Juss.)
Understanding the critical stages in ovule development is essential for optimizing in vitro seed propagation, cryopreservation, and indirect somatic plant embryogenesis. We studied the main stages of the megasporogenesis and megagametogenesis of <i>Coelogyne speciosa</i> subsp. <i>...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | International Journal of Plant Biology |
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| Online Access: | https://www.mdpi.com/2037-0164/14/1/16 |
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| author | Galina Kolomeitseva Vladimir Koval Andrey Ryabchenko Alexander Babosha |
| author_facet | Galina Kolomeitseva Vladimir Koval Andrey Ryabchenko Alexander Babosha |
| author_sort | Galina Kolomeitseva |
| collection | DOAJ |
| description | Understanding the critical stages in ovule development is essential for optimizing in vitro seed propagation, cryopreservation, and indirect somatic plant embryogenesis. We studied the main stages of the megasporogenesis and megagametogenesis of <i>Coelogyne speciosa</i> subsp. <i>fimbriata</i> (J.J.Sm.) Gravendeel by confocal laser scanning microscopy. The ovule is anatropic, tenuinucellate, bitegmal, and funicular. The embryo sac is monosporic, Polygonum-type, bipolar, and usually 8-nuclear, but in cases of a reduction in the last mitotic division, it is 6-nuclear. Endosperm formation was not detected. The formation of a functional megaspore occurred 65–70 days after pollination (DAP), while the formation of a mature embryo sac and fertilization occurred after 85–100 DAP. |
| first_indexed | 2024-03-11T06:24:34Z |
| format | Article |
| id | doaj.art-e9a155dd19994b578a4a7402fcbb5c86 |
| institution | Directory Open Access Journal |
| issn | 2037-0164 |
| language | English |
| last_indexed | 2024-03-11T06:24:34Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Plant Biology |
| spelling | doaj.art-e9a155dd19994b578a4a7402fcbb5c862023-11-17T11:42:43ZengMDPI AGInternational Journal of Plant Biology2037-01642023-01-0114119019810.3390/ijpb14010016Megasporogenesis and Megagametogenesis in <i>Coelogyne speciosa</i> subsp. <i>fimbriata</i> (J.J.Sm.) Gravendeel (Orchidaceae Juss.)Galina Kolomeitseva0Vladimir Koval1Andrey Ryabchenko2Alexander Babosha3Tsitsin Main Botanical Garden, Russian Academy of Sciences, 127276 Moscow, RussiaTsitsin Main Botanical Garden, Russian Academy of Sciences, 127276 Moscow, RussiaTsitsin Main Botanical Garden, Russian Academy of Sciences, 127276 Moscow, RussiaTsitsin Main Botanical Garden, Russian Academy of Sciences, 127276 Moscow, RussiaUnderstanding the critical stages in ovule development is essential for optimizing in vitro seed propagation, cryopreservation, and indirect somatic plant embryogenesis. We studied the main stages of the megasporogenesis and megagametogenesis of <i>Coelogyne speciosa</i> subsp. <i>fimbriata</i> (J.J.Sm.) Gravendeel by confocal laser scanning microscopy. The ovule is anatropic, tenuinucellate, bitegmal, and funicular. The embryo sac is monosporic, Polygonum-type, bipolar, and usually 8-nuclear, but in cases of a reduction in the last mitotic division, it is 6-nuclear. Endosperm formation was not detected. The formation of a functional megaspore occurred 65–70 days after pollination (DAP), while the formation of a mature embryo sac and fertilization occurred after 85–100 DAP.https://www.mdpi.com/2037-0164/14/1/16embryo sacfemale gametophytemegasporeovule |
| spellingShingle | Galina Kolomeitseva Vladimir Koval Andrey Ryabchenko Alexander Babosha Megasporogenesis and Megagametogenesis in <i>Coelogyne speciosa</i> subsp. <i>fimbriata</i> (J.J.Sm.) Gravendeel (Orchidaceae Juss.) International Journal of Plant Biology embryo sac female gametophyte megaspore ovule |
| title | Megasporogenesis and Megagametogenesis in <i>Coelogyne speciosa</i> subsp. <i>fimbriata</i> (J.J.Sm.) Gravendeel (Orchidaceae Juss.) |
| title_full | Megasporogenesis and Megagametogenesis in <i>Coelogyne speciosa</i> subsp. <i>fimbriata</i> (J.J.Sm.) Gravendeel (Orchidaceae Juss.) |
| title_fullStr | Megasporogenesis and Megagametogenesis in <i>Coelogyne speciosa</i> subsp. <i>fimbriata</i> (J.J.Sm.) Gravendeel (Orchidaceae Juss.) |
| title_full_unstemmed | Megasporogenesis and Megagametogenesis in <i>Coelogyne speciosa</i> subsp. <i>fimbriata</i> (J.J.Sm.) Gravendeel (Orchidaceae Juss.) |
| title_short | Megasporogenesis and Megagametogenesis in <i>Coelogyne speciosa</i> subsp. <i>fimbriata</i> (J.J.Sm.) Gravendeel (Orchidaceae Juss.) |
| title_sort | megasporogenesis and megagametogenesis in i coelogyne speciosa i subsp i fimbriata i j j sm gravendeel orchidaceae juss |
| topic | embryo sac female gametophyte megaspore ovule |
| url | https://www.mdpi.com/2037-0164/14/1/16 |
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