Evaluation of matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) for the Identification of Group B Streptococcus
Abstract Objective Group B Streptococcus (GBS) is a leading cause of neonatal meningitis and sepsis worldwide. Intrapartum antibiotics given to women carrying GBS are an effective means of reducing disease in the first week of life. Rapid and reliable tests are needed to accurately identify GBS from...
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| Format: | Article |
| Language: | English |
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BMC
2019-02-01
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| Series: | BMC Research Notes |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13104-019-4119-1 |
| _version_ | 1818037712339337216 |
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| author | Ka-Ning To Emma Cornwell Roger Daniel Sweenie Goonesekera Elita Jauneikaite Victoria Chalker Kirsty Le Doare |
| author_facet | Ka-Ning To Emma Cornwell Roger Daniel Sweenie Goonesekera Elita Jauneikaite Victoria Chalker Kirsty Le Doare |
| author_sort | Ka-Ning To |
| collection | DOAJ |
| description | Abstract Objective Group B Streptococcus (GBS) is a leading cause of neonatal meningitis and sepsis worldwide. Intrapartum antibiotics given to women carrying GBS are an effective means of reducing disease in the first week of life. Rapid and reliable tests are needed to accurately identify GBS from these women for timely intrapartum antibiotic administration to prevent neonatal disease. Many laboratories now use matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) by direct plating or cell lysis for the identification of GBS isolates. The cell lysis step increases time to results for clinical samples and is more complex to perform. Therefore, we seek to evaluate the sensitivity and specificity of the quicker and more rapid direct plating method in identifying GBS. Results We directly compared swab isolates analysed by both direct plating and cell lysis method and demonstrated that direct plating has a sensitivity and specificity of 0.97 and 1, respectively, compared to an additional cell lysis step. We demonstrated that MALDI-TOF MS can be successfully used for batch processing by the direct plating method which saves time. These results are reassuring for laboratories worldwide who seek to identify GBS from swabs samples as quickly as possible. |
| first_indexed | 2024-12-10T07:31:12Z |
| format | Article |
| id | doaj.art-e9a194595e5f47ff98bbcc3d0911aa3e |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-10T07:31:12Z |
| publishDate | 2019-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-e9a194595e5f47ff98bbcc3d0911aa3e2022-12-22T01:57:34ZengBMCBMC Research Notes1756-05002019-02-011211610.1186/s13104-019-4119-1Evaluation of matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) for the Identification of Group B StreptococcusKa-Ning To0Emma Cornwell1Roger Daniel2Sweenie Goonesekera3Elita Jauneikaite4Victoria Chalker5Kirsty Le Doare6Section of Paediatrics, Department of Medicine, Imperial College LondonSection of Paediatrics, Department of Medicine, Imperial College LondonPublic Health EnglandInfection and Immunity, Department of Microbiology, Charing Cross HospitalDepartment of Infectious Disease Epidemiology, Imperial College LondonPublic Health EnglandSection of Paediatrics, Department of Medicine, Imperial College LondonAbstract Objective Group B Streptococcus (GBS) is a leading cause of neonatal meningitis and sepsis worldwide. Intrapartum antibiotics given to women carrying GBS are an effective means of reducing disease in the first week of life. Rapid and reliable tests are needed to accurately identify GBS from these women for timely intrapartum antibiotic administration to prevent neonatal disease. Many laboratories now use matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) by direct plating or cell lysis for the identification of GBS isolates. The cell lysis step increases time to results for clinical samples and is more complex to perform. Therefore, we seek to evaluate the sensitivity and specificity of the quicker and more rapid direct plating method in identifying GBS. Results We directly compared swab isolates analysed by both direct plating and cell lysis method and demonstrated that direct plating has a sensitivity and specificity of 0.97 and 1, respectively, compared to an additional cell lysis step. We demonstrated that MALDI-TOF MS can be successfully used for batch processing by the direct plating method which saves time. These results are reassuring for laboratories worldwide who seek to identify GBS from swabs samples as quickly as possible.http://link.springer.com/article/10.1186/s13104-019-4119-1Group B StreptococcusStreptococcus agalactiaeRapid diagnosticsMALDI-TOF MS |
| spellingShingle | Ka-Ning To Emma Cornwell Roger Daniel Sweenie Goonesekera Elita Jauneikaite Victoria Chalker Kirsty Le Doare Evaluation of matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) for the Identification of Group B Streptococcus BMC Research Notes Group B Streptococcus Streptococcus agalactiae Rapid diagnostics MALDI-TOF MS |
| title | Evaluation of matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) for the Identification of Group B Streptococcus |
| title_full | Evaluation of matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) for the Identification of Group B Streptococcus |
| title_fullStr | Evaluation of matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) for the Identification of Group B Streptococcus |
| title_full_unstemmed | Evaluation of matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) for the Identification of Group B Streptococcus |
| title_short | Evaluation of matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) for the Identification of Group B Streptococcus |
| title_sort | evaluation of matrix assisted laser desorption ionisation time of flight mass spectrometry maldi tof ms for the identification of group b streptococcus |
| topic | Group B Streptococcus Streptococcus agalactiae Rapid diagnostics MALDI-TOF MS |
| url | http://link.springer.com/article/10.1186/s13104-019-4119-1 |
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