ATP-free in vitro biotransformation of starch-derived maltodextrin into poly-3-hydroxybutyrate via acetyl-CoA
Abstract In vitro biotransformation (ivBT) facilitated by in vitro synthetic enzymatic biosystems (ivSEBs) has emerged as a highly promising biosynthetic platform. Several ivSEBs have been constructed to produce poly-3-hydroxybutyrate (PHB) via acetyl-coenzyme A (acetyl-CoA). However, some systems a...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-04-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-46871-y |
| _version_ | 1797199365183373312 |
|---|---|
| author | Xinlei Wei Xue Yang Congcong Hu Qiangzi Li Qianqian Liu Yue Wu Leipeng Xie Xiao Ning Fei Li Tao Cai Zhiguang Zhu Yi-Heng P. Job Zhang Yanfei Zhang Xuejun Chen Chun You |
| author_facet | Xinlei Wei Xue Yang Congcong Hu Qiangzi Li Qianqian Liu Yue Wu Leipeng Xie Xiao Ning Fei Li Tao Cai Zhiguang Zhu Yi-Heng P. Job Zhang Yanfei Zhang Xuejun Chen Chun You |
| author_sort | Xinlei Wei |
| collection | DOAJ |
| description | Abstract In vitro biotransformation (ivBT) facilitated by in vitro synthetic enzymatic biosystems (ivSEBs) has emerged as a highly promising biosynthetic platform. Several ivSEBs have been constructed to produce poly-3-hydroxybutyrate (PHB) via acetyl-coenzyme A (acetyl-CoA). However, some systems are hindered by their reliance on costly ATP, limiting their practicality. This study presents the design of an ATP-free ivSEB for one-pot PHB biosynthesis via acetyl-CoA utilizing starch-derived maltodextrin as the sole substrate. Stoichiometric analysis indicates this ivSEB can self-maintain NADP+/NADPH balance and achieve a theoretical molar yield of 133.3%. Leveraging simple one-pot reactions, our ivSEBs achieved a near-theoretical molar yield of 125.5%, the highest PHB titer (208.3 mM, approximately 17.9 g/L) and the fastest PHB production rate (9.4 mM/h, approximately 0.8 g/L/h) among all the reported ivSEBs to date, and demonstrated easy scalability. This study unveils the promising potential of ivBT for the industrial-scale production of PHB and other acetyl-CoA-derived chemicals from starch. |
| first_indexed | 2024-04-24T07:14:36Z |
| format | Article |
| id | doaj.art-e9a3f667f71d4ff69416e5f6e82bc8d3 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-24T07:14:36Z |
| publishDate | 2024-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-e9a3f667f71d4ff69416e5f6e82bc8d32024-04-21T11:23:58ZengNature PortfolioNature Communications2041-17232024-04-0115111410.1038/s41467-024-46871-yATP-free in vitro biotransformation of starch-derived maltodextrin into poly-3-hydroxybutyrate via acetyl-CoAXinlei Wei0Xue Yang1Congcong Hu2Qiangzi Li3Qianqian Liu4Yue Wu5Leipeng Xie6Xiao Ning7Fei Li8Tao Cai9Zhiguang Zhu10Yi-Heng P. Job Zhang11Yanfei Zhang12Xuejun Chen13Chun You14In vitro Synthetic Biology Center, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic AreaTianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic AreaIn vitro Synthetic Biology Center, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic AreaIn vitro Synthetic Biology Center, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic AreaTianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic AreaTianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic AreaIn vitro Synthetic Biology Center, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic AreaIn vitro Synthetic Biology Center, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic AreaIn vitro Synthetic Biology Center, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic AreaTianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic AreaIn vitro Synthetic Biology Center, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic AreaIn vitro Synthetic Biology Center, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic AreaTianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic AreaTianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic AreaIn vitro Synthetic Biology Center, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic AreaAbstract In vitro biotransformation (ivBT) facilitated by in vitro synthetic enzymatic biosystems (ivSEBs) has emerged as a highly promising biosynthetic platform. Several ivSEBs have been constructed to produce poly-3-hydroxybutyrate (PHB) via acetyl-coenzyme A (acetyl-CoA). However, some systems are hindered by their reliance on costly ATP, limiting their practicality. This study presents the design of an ATP-free ivSEB for one-pot PHB biosynthesis via acetyl-CoA utilizing starch-derived maltodextrin as the sole substrate. Stoichiometric analysis indicates this ivSEB can self-maintain NADP+/NADPH balance and achieve a theoretical molar yield of 133.3%. Leveraging simple one-pot reactions, our ivSEBs achieved a near-theoretical molar yield of 125.5%, the highest PHB titer (208.3 mM, approximately 17.9 g/L) and the fastest PHB production rate (9.4 mM/h, approximately 0.8 g/L/h) among all the reported ivSEBs to date, and demonstrated easy scalability. This study unveils the promising potential of ivBT for the industrial-scale production of PHB and other acetyl-CoA-derived chemicals from starch.https://doi.org/10.1038/s41467-024-46871-y |
| spellingShingle | Xinlei Wei Xue Yang Congcong Hu Qiangzi Li Qianqian Liu Yue Wu Leipeng Xie Xiao Ning Fei Li Tao Cai Zhiguang Zhu Yi-Heng P. Job Zhang Yanfei Zhang Xuejun Chen Chun You ATP-free in vitro biotransformation of starch-derived maltodextrin into poly-3-hydroxybutyrate via acetyl-CoA Nature Communications |
| title | ATP-free in vitro biotransformation of starch-derived maltodextrin into poly-3-hydroxybutyrate via acetyl-CoA |
| title_full | ATP-free in vitro biotransformation of starch-derived maltodextrin into poly-3-hydroxybutyrate via acetyl-CoA |
| title_fullStr | ATP-free in vitro biotransformation of starch-derived maltodextrin into poly-3-hydroxybutyrate via acetyl-CoA |
| title_full_unstemmed | ATP-free in vitro biotransformation of starch-derived maltodextrin into poly-3-hydroxybutyrate via acetyl-CoA |
| title_short | ATP-free in vitro biotransformation of starch-derived maltodextrin into poly-3-hydroxybutyrate via acetyl-CoA |
| title_sort | atp free in vitro biotransformation of starch derived maltodextrin into poly 3 hydroxybutyrate via acetyl coa |
| url | https://doi.org/10.1038/s41467-024-46871-y |
| work_keys_str_mv | AT xinleiwei atpfreeinvitrobiotransformationofstarchderivedmaltodextrinintopoly3hydroxybutyrateviaacetylcoa AT xueyang atpfreeinvitrobiotransformationofstarchderivedmaltodextrinintopoly3hydroxybutyrateviaacetylcoa AT congconghu atpfreeinvitrobiotransformationofstarchderivedmaltodextrinintopoly3hydroxybutyrateviaacetylcoa AT qiangzili atpfreeinvitrobiotransformationofstarchderivedmaltodextrinintopoly3hydroxybutyrateviaacetylcoa AT qianqianliu atpfreeinvitrobiotransformationofstarchderivedmaltodextrinintopoly3hydroxybutyrateviaacetylcoa AT yuewu atpfreeinvitrobiotransformationofstarchderivedmaltodextrinintopoly3hydroxybutyrateviaacetylcoa AT leipengxie atpfreeinvitrobiotransformationofstarchderivedmaltodextrinintopoly3hydroxybutyrateviaacetylcoa AT xiaoning atpfreeinvitrobiotransformationofstarchderivedmaltodextrinintopoly3hydroxybutyrateviaacetylcoa AT feili atpfreeinvitrobiotransformationofstarchderivedmaltodextrinintopoly3hydroxybutyrateviaacetylcoa AT taocai atpfreeinvitrobiotransformationofstarchderivedmaltodextrinintopoly3hydroxybutyrateviaacetylcoa AT zhiguangzhu atpfreeinvitrobiotransformationofstarchderivedmaltodextrinintopoly3hydroxybutyrateviaacetylcoa AT yihengpjobzhang atpfreeinvitrobiotransformationofstarchderivedmaltodextrinintopoly3hydroxybutyrateviaacetylcoa AT yanfeizhang atpfreeinvitrobiotransformationofstarchderivedmaltodextrinintopoly3hydroxybutyrateviaacetylcoa AT xuejunchen atpfreeinvitrobiotransformationofstarchderivedmaltodextrinintopoly3hydroxybutyrateviaacetylcoa AT chunyou atpfreeinvitrobiotransformationofstarchderivedmaltodextrinintopoly3hydroxybutyrateviaacetylcoa |