Antibacterial effects of mangrove ethanolic leaf extract against zoonotic fish pathogen Salmonella arizonae
The emergence of multiple drug-resistant pathogens, affecting aquaculture and public health, has put the spotlight on alternative medicine research. This study was conducted to evaluate the In vitro and In vivo antibacterial activity of mangrove ethanolic leaf extract (MLEE) against Salmonella arizo...
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| Format: | Article |
| Language: | English |
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BdFISH
2021-08-01
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| Series: | Journal of Fisheries |
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| Online Access: | https://journal.bdfish.org/index.php/fisheries/article/view/260 |
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| author | Jomel S Limbago Josette Sosas Angelie A Gente Parif Maderse Marjorie M Rocamora Dennis K Gomez |
| author_facet | Jomel S Limbago Josette Sosas Angelie A Gente Parif Maderse Marjorie M Rocamora Dennis K Gomez |
| author_sort | Jomel S Limbago |
| collection | DOAJ |
| description | The emergence of multiple drug-resistant pathogens, affecting aquaculture and public health, has put the spotlight on alternative medicine research. This study was conducted to evaluate the In vitro and In vivo antibacterial activity of mangrove ethanolic leaf extract (MLEE) against Salmonella arizonae isolated from Carassius auratus. In vitro, antimicrobial activity of 10 mangrove species and 13 commercial antibiotics were determined using the agar diffusion method. MLEE with the highest antimicrobial activity were subjected to qualitative phytochemical tests and bioassay experiments. In vivo antibacterial activity of MLEE was assessed using C. auratus intraperitoneally injected with S. arizonae. Results showed that Sonneratia alba has the highest antimicrobial activity against S. arizonae followed by Avicennia marina, A. officinalis, Sonneratia ovata, Rhizophora mucronata, Excoecaria agallocha, and Bruguiera cylindrica. However, bacterial isolate was resistant to A. rhumpiana, Scyphiphora hydrophyllacea, and Laguncularia racemosa. Interestingly, S. alba has comparable antimicrobial activity with amoxicillin, trimethoprim, novobiocin, and cefixime. The activity of S. alba could be attributed to the presence of flavonoids, saponin, sterols, tannin, and terpenoids. Moreover, S. alba has reduced and delayed the onset of goldfish mortality infected with S. arizonae. Based on these findings, the S. alba MLEE, is a potential antimicrobial resource against S. arizonae. |
| first_indexed | 2024-12-13T10:25:04Z |
| format | Article |
| id | doaj.art-e9ab31d38228425c9c817b2566da6fb9 |
| institution | Directory Open Access Journal |
| issn | 2311-729X 2311-3111 |
| language | English |
| last_indexed | 2024-12-13T10:25:04Z |
| publishDate | 2021-08-01 |
| publisher | BdFISH |
| record_format | Article |
| series | Journal of Fisheries |
| spelling | doaj.art-e9ab31d38228425c9c817b2566da6fb92022-12-21T23:51:05ZengBdFISHJournal of Fisheries2311-729X2311-31112021-08-0192Antibacterial effects of mangrove ethanolic leaf extract against zoonotic fish pathogen Salmonella arizonaeJomel S Limbago0Josette Sosas1Angelie A Gente2Parif Maderse3Marjorie M Rocamora4Dennis K Gomez5Fisheries and Aquatic Sciences Department, Cavite State University – Naic, Bucana Malake, Naic, Cavite, Philippines; College of Fisheries and Aquatic Sciences, Iloilo State College of Fisheries, Tiwi, Barotac Nuevo, Iloilo, PhilippinesCollege of Fisheries and Aquatic Sciences, Iloilo State College of Fisheries, Tiwi, Barotac Nuevo, Iloilo, Philippines; Bureau of Fisheries and Aquatic Sciences, Regional Office VI, Department of Agriculture, Iloilo City, PhilippinesCollege of Fisheries and Aquatic Sciences, Iloilo State College of Fisheries, Tiwi, Barotac Nuevo, Iloilo, PhilippinesCollege of Fisheries and Aquatic Sciences, Iloilo State College of Fisheries, Tiwi, Barotac Nuevo, Iloilo, Philippines; National Marine Fisheries Development Center, MCS and Fishing Technology Station, Bureau of Fisheries and Aquatic Resources, Navotas City, Philippines College of Fisheries and Aquatic Sciences, Iloilo State College of Fisheries, Tiwi, Barotac Nuevo, Iloilo, Philippines; Aquaculture Department, Southeast Asian Fisheries Development Center, Tigbauan, Iloilo, PhilippinesCollege of Fisheries and Aquatic Sciences, Iloilo State College of Fisheries, Tiwi, Barotac Nuevo, Iloilo, Philippines; Fish Health Laboratory, College of Fisheries and Aquatic Sciences, Iloilo State College of Fisheries, Tiwi, Barotac Nuevo, Iloilo, PhilippinesThe emergence of multiple drug-resistant pathogens, affecting aquaculture and public health, has put the spotlight on alternative medicine research. This study was conducted to evaluate the In vitro and In vivo antibacterial activity of mangrove ethanolic leaf extract (MLEE) against Salmonella arizonae isolated from Carassius auratus. In vitro, antimicrobial activity of 10 mangrove species and 13 commercial antibiotics were determined using the agar diffusion method. MLEE with the highest antimicrobial activity were subjected to qualitative phytochemical tests and bioassay experiments. In vivo antibacterial activity of MLEE was assessed using C. auratus intraperitoneally injected with S. arizonae. Results showed that Sonneratia alba has the highest antimicrobial activity against S. arizonae followed by Avicennia marina, A. officinalis, Sonneratia ovata, Rhizophora mucronata, Excoecaria agallocha, and Bruguiera cylindrica. However, bacterial isolate was resistant to A. rhumpiana, Scyphiphora hydrophyllacea, and Laguncularia racemosa. Interestingly, S. alba has comparable antimicrobial activity with amoxicillin, trimethoprim, novobiocin, and cefixime. The activity of S. alba could be attributed to the presence of flavonoids, saponin, sterols, tannin, and terpenoids. Moreover, S. alba has reduced and delayed the onset of goldfish mortality infected with S. arizonae. Based on these findings, the S. alba MLEE, is a potential antimicrobial resource against S. arizonae.https://journal.bdfish.org/index.php/fisheries/article/view/260AntibacterialIn vitroIn vivomangrovesphytochemicalszone of inhibition |
| spellingShingle | Jomel S Limbago Josette Sosas Angelie A Gente Parif Maderse Marjorie M Rocamora Dennis K Gomez Antibacterial effects of mangrove ethanolic leaf extract against zoonotic fish pathogen Salmonella arizonae Journal of Fisheries Antibacterial In vitro In vivo mangroves phytochemicals zone of inhibition |
| title | Antibacterial effects of mangrove ethanolic leaf extract against zoonotic fish pathogen Salmonella arizonae |
| title_full | Antibacterial effects of mangrove ethanolic leaf extract against zoonotic fish pathogen Salmonella arizonae |
| title_fullStr | Antibacterial effects of mangrove ethanolic leaf extract against zoonotic fish pathogen Salmonella arizonae |
| title_full_unstemmed | Antibacterial effects of mangrove ethanolic leaf extract against zoonotic fish pathogen Salmonella arizonae |
| title_short | Antibacterial effects of mangrove ethanolic leaf extract against zoonotic fish pathogen Salmonella arizonae |
| title_sort | antibacterial effects of mangrove ethanolic leaf extract against zoonotic fish pathogen salmonella arizonae |
| topic | Antibacterial In vitro In vivo mangroves phytochemicals zone of inhibition |
| url | https://journal.bdfish.org/index.php/fisheries/article/view/260 |
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