Vesicular Glutamate Transporters in Astrocytes as Potential New Therapeutic Targets: Astrocyte-targeted Viral Vectors Expressing Inhibitory Nanobodies
Astrocytes, the main Central Nervous System (CNS) glial cell type, actively release transmitters, including glutamate, and thereby participate in physiological brain information processing. However, dysregulated transmitter release from astrocytes can contribute to CNS disease pathogenesis and prog...
| Main Authors: | , |
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| Format: | Article |
| Language: | deu |
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Swiss Chemical Society
2022-12-01
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| Series: | CHIMIA |
| Subjects: | |
| Online Access: | https://chimia.ch/chimia/article/view/6172 |
| _version_ | 1811166371382296576 |
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| author | Roberta de Ceglia Andrea Volterra |
| author_facet | Roberta de Ceglia Andrea Volterra |
| author_sort | Roberta de Ceglia |
| collection | DOAJ |
| description |
Astrocytes, the main Central Nervous System (CNS) glial cell type, actively release transmitters, including glutamate, and thereby participate in physiological brain information processing. However, dysregulated transmitter release from astrocytes can contribute to CNS disease pathogenesis and progression. Therefore, targeting astrocyte glutamate release is a promising new therapeutic strategy in hyper-glutamatergic brain conditions, as it does not directly block glutamatergic neurotransmission. Basing on the evidence that astrocytes express Vesicular Glutamate Transporters (VGLUT), in collaboration with other NCCR TransCure partners, we developed an innovative approach for astrocyte-selective delivery of nanobodies inhibiting VGLUT. We inserted the anti-VGLUT nanobody constructs in astrocyte-directed viral vectors that were administered peripherally, crossed the blood-brain-barrier and led to successful cell-specific CNS-wide expression of the nanobodies.
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| first_indexed | 2024-04-10T15:51:11Z |
| format | Article |
| id | doaj.art-e9ae9b316c844ff79c5dd4c7ec98fbb9 |
| institution | Directory Open Access Journal |
| issn | 0009-4293 2673-2424 |
| language | deu |
| last_indexed | 2024-04-10T15:51:11Z |
| publishDate | 2022-12-01 |
| publisher | Swiss Chemical Society |
| record_format | Article |
| series | CHIMIA |
| spelling | doaj.art-e9ae9b316c844ff79c5dd4c7ec98fbb92023-02-12T01:02:19ZdeuSwiss Chemical SocietyCHIMIA0009-42932673-24242022-12-01761210.2533/chimia.2022.1033Vesicular Glutamate Transporters in Astrocytes as Potential New Therapeutic Targets: Astrocyte-targeted Viral Vectors Expressing Inhibitory NanobodiesRoberta de Ceglia0Andrea Volterra1Department of Fundamental Neurosciences, University of LausanneDepartment of Fundamental Neutrosciences, University of Lausanne; Wyss Center, Chemin des Mines 9, Geneva Astrocytes, the main Central Nervous System (CNS) glial cell type, actively release transmitters, including glutamate, and thereby participate in physiological brain information processing. However, dysregulated transmitter release from astrocytes can contribute to CNS disease pathogenesis and progression. Therefore, targeting astrocyte glutamate release is a promising new therapeutic strategy in hyper-glutamatergic brain conditions, as it does not directly block glutamatergic neurotransmission. Basing on the evidence that astrocytes express Vesicular Glutamate Transporters (VGLUT), in collaboration with other NCCR TransCure partners, we developed an innovative approach for astrocyte-selective delivery of nanobodies inhibiting VGLUT. We inserted the anti-VGLUT nanobody constructs in astrocyte-directed viral vectors that were administered peripherally, crossed the blood-brain-barrier and led to successful cell-specific CNS-wide expression of the nanobodies. https://chimia.ch/chimia/article/view/6172AstrocyteCell-specific viral vectorsNanobodyVGLUT |
| spellingShingle | Roberta de Ceglia Andrea Volterra Vesicular Glutamate Transporters in Astrocytes as Potential New Therapeutic Targets: Astrocyte-targeted Viral Vectors Expressing Inhibitory Nanobodies CHIMIA Astrocyte Cell-specific viral vectors Nanobody VGLUT |
| title | Vesicular Glutamate Transporters in Astrocytes as Potential New Therapeutic Targets: Astrocyte-targeted Viral Vectors Expressing Inhibitory Nanobodies |
| title_full | Vesicular Glutamate Transporters in Astrocytes as Potential New Therapeutic Targets: Astrocyte-targeted Viral Vectors Expressing Inhibitory Nanobodies |
| title_fullStr | Vesicular Glutamate Transporters in Astrocytes as Potential New Therapeutic Targets: Astrocyte-targeted Viral Vectors Expressing Inhibitory Nanobodies |
| title_full_unstemmed | Vesicular Glutamate Transporters in Astrocytes as Potential New Therapeutic Targets: Astrocyte-targeted Viral Vectors Expressing Inhibitory Nanobodies |
| title_short | Vesicular Glutamate Transporters in Astrocytes as Potential New Therapeutic Targets: Astrocyte-targeted Viral Vectors Expressing Inhibitory Nanobodies |
| title_sort | vesicular glutamate transporters in astrocytes as potential new therapeutic targets astrocyte targeted viral vectors expressing inhibitory nanobodies |
| topic | Astrocyte Cell-specific viral vectors Nanobody VGLUT |
| url | https://chimia.ch/chimia/article/view/6172 |
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