Utility of fungal polymerase chain reaction on nasal swab samples in the diagnosis and monitoring of sinonasal aspergillosis in dogs
Abstract Background In dogs with sinonasal aspergillosis (SNA) the utility of PCR in the diagnosis and monitoring of the disease after treatment has not been assessed. Objectives To evaluate the presence of fungal DNA using quantitative PCR targeting Aspergillus fumigatus (Aspfum) and Aspergillus sp...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-07-01
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| Series: | Journal of Veterinary Internal Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/jvim.16441 |
| _version_ | 1811288032756629504 |
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| author | Tom Biénès Emilie Vangrinsven Aline Fastrès Mutien‐Marie Garigliany Frédéric Billen Cécile Clercx |
| author_facet | Tom Biénès Emilie Vangrinsven Aline Fastrès Mutien‐Marie Garigliany Frédéric Billen Cécile Clercx |
| author_sort | Tom Biénès |
| collection | DOAJ |
| description | Abstract Background In dogs with sinonasal aspergillosis (SNA) the utility of PCR in the diagnosis and monitoring of the disease after treatment has not been assessed. Objectives To evaluate the presence of fungal DNA using quantitative PCR targeting Aspergillus fumigatus (Aspfum) and Aspergillus spp. (PanAsp), and PCR targeting multiple fungal species (PanFun), in samples obtained from nasal cavities of dogs with SNA, other nasal diseases and healthy dogs. Animals Sixty‐two dogs including 20 with SNA, 12 with cured SNA (of which 10 are from the SNA group), 20 dogs with Non‐SNA nasal disease, and 20 healthy dogs. Methods Prospective cross‐sectional study. Aspfum, PanAsp, and PanFun were performed on blindly collected nasal swabs obtained in anesthetized dogs. Results In SNA dogs, Aspfum and PanAsp were positive in 13/20 and 14/20 dogs. In all dogs in the 3 other groups, A. fumigatus DNA was not detected using Aspfum. PanAsp was positive in 3 non‐SNA dogs: 1 with cured SNA and 2 with Non‐SNA nasal disease. A Ct cut‐off value of 33.3 for Aspfum demonstrated 65% sensitivity and 100% specificity. A Ct cut‐off value of 34.5 for PanAsp demonstrated 70% sensitivity and 96.2% specificity. PanFun was positive in 16/20, 12/12, 19/20, and 7/20 dogs in the SNA, cured SNA, Non‐SNA, and healthy groups, respectively. Conclusion and Clinical Importance Aspfum and PanAsp on blindly collected nasal swabs can be useful for the detection of SNA at diagnosis and at cure, especially when more invasive methods are not available. |
| first_indexed | 2024-04-13T03:28:53Z |
| format | Article |
| id | doaj.art-e9b3100c88db437088b2ff4deb27c378 |
| institution | Directory Open Access Journal |
| issn | 0891-6640 1939-1676 |
| language | English |
| last_indexed | 2024-04-13T03:28:53Z |
| publishDate | 2022-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Veterinary Internal Medicine |
| spelling | doaj.art-e9b3100c88db437088b2ff4deb27c3782022-12-22T03:04:33ZengWileyJournal of Veterinary Internal Medicine0891-66401939-16762022-07-013641295130210.1111/jvim.16441Utility of fungal polymerase chain reaction on nasal swab samples in the diagnosis and monitoring of sinonasal aspergillosis in dogsTom Biénès0Emilie Vangrinsven1Aline Fastrès2Mutien‐Marie Garigliany3Frédéric Billen4Cécile Clercx5Department of Clinical Sciences, Faculty of Veterinary Medicine, FARAH University of Liège Liège BelgiumDepartment of Clinical Sciences, Faculty of Veterinary Medicine, FARAH University of Liège Liège BelgiumDepartment of Clinical Sciences, Faculty of Veterinary Medicine, FARAH University of Liège Liège BelgiumDepartment of Veterinary Pathology, Faculty of Veterinary Medicine, FARAH University of Liège Liège BelgiumDepartment of Clinical Sciences, Faculty of Veterinary Medicine, FARAH University of Liège Liège BelgiumDepartment of Clinical Sciences, Faculty of Veterinary Medicine, FARAH University of Liège Liège BelgiumAbstract Background In dogs with sinonasal aspergillosis (SNA) the utility of PCR in the diagnosis and monitoring of the disease after treatment has not been assessed. Objectives To evaluate the presence of fungal DNA using quantitative PCR targeting Aspergillus fumigatus (Aspfum) and Aspergillus spp. (PanAsp), and PCR targeting multiple fungal species (PanFun), in samples obtained from nasal cavities of dogs with SNA, other nasal diseases and healthy dogs. Animals Sixty‐two dogs including 20 with SNA, 12 with cured SNA (of which 10 are from the SNA group), 20 dogs with Non‐SNA nasal disease, and 20 healthy dogs. Methods Prospective cross‐sectional study. Aspfum, PanAsp, and PanFun were performed on blindly collected nasal swabs obtained in anesthetized dogs. Results In SNA dogs, Aspfum and PanAsp were positive in 13/20 and 14/20 dogs. In all dogs in the 3 other groups, A. fumigatus DNA was not detected using Aspfum. PanAsp was positive in 3 non‐SNA dogs: 1 with cured SNA and 2 with Non‐SNA nasal disease. A Ct cut‐off value of 33.3 for Aspfum demonstrated 65% sensitivity and 100% specificity. A Ct cut‐off value of 34.5 for PanAsp demonstrated 70% sensitivity and 96.2% specificity. PanFun was positive in 16/20, 12/12, 19/20, and 7/20 dogs in the SNA, cured SNA, Non‐SNA, and healthy groups, respectively. Conclusion and Clinical Importance Aspfum and PanAsp on blindly collected nasal swabs can be useful for the detection of SNA at diagnosis and at cure, especially when more invasive methods are not available.https://doi.org/10.1111/jvim.16441aspergillosisdiagnosisdogsnasal swabsPCR |
| spellingShingle | Tom Biénès Emilie Vangrinsven Aline Fastrès Mutien‐Marie Garigliany Frédéric Billen Cécile Clercx Utility of fungal polymerase chain reaction on nasal swab samples in the diagnosis and monitoring of sinonasal aspergillosis in dogs Journal of Veterinary Internal Medicine aspergillosis diagnosis dogs nasal swabs PCR |
| title | Utility of fungal polymerase chain reaction on nasal swab samples in the diagnosis and monitoring of sinonasal aspergillosis in dogs |
| title_full | Utility of fungal polymerase chain reaction on nasal swab samples in the diagnosis and monitoring of sinonasal aspergillosis in dogs |
| title_fullStr | Utility of fungal polymerase chain reaction on nasal swab samples in the diagnosis and monitoring of sinonasal aspergillosis in dogs |
| title_full_unstemmed | Utility of fungal polymerase chain reaction on nasal swab samples in the diagnosis and monitoring of sinonasal aspergillosis in dogs |
| title_short | Utility of fungal polymerase chain reaction on nasal swab samples in the diagnosis and monitoring of sinonasal aspergillosis in dogs |
| title_sort | utility of fungal polymerase chain reaction on nasal swab samples in the diagnosis and monitoring of sinonasal aspergillosis in dogs |
| topic | aspergillosis diagnosis dogs nasal swabs PCR |
| url | https://doi.org/10.1111/jvim.16441 |
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