Effects of combined 5-Fluorouracil and ZnO NPs on human breast cancer MCF-7 Cells: P53 gene expression, Bcl-2 signaling pathway, and invasion activity
Objective(s): The significant contribution of nanoparticles to cancer treatment has attracted therapeutic attention. The present study aimed to evaluate the synergistic effects of 5-fluorouracil (5-FU) and zinc oxide nanoparticles (ZnO NPs) as multimodal drug delivery on human breast cancer MCF-7 cel...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2019-07-01
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| Series: | Nanomedicine Journal |
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| Online Access: | http://nmj.mums.ac.ir/article_13111_60382b380848d83580e1426dce38618b.pdf |
| _version_ | 1828930527648808960 |
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| author | Safoura Hoseinzadeh Elham Raeisi Yves Lemoigne Esfandiar Heidarian |
| author_facet | Safoura Hoseinzadeh Elham Raeisi Yves Lemoigne Esfandiar Heidarian |
| author_sort | Safoura Hoseinzadeh |
| collection | DOAJ |
| description | Objective(s): The significant contribution of nanoparticles to cancer treatment has attracted therapeutic attention. The present study aimed to evaluate the synergistic effects of 5-fluorouracil (5-FU) and zinc oxide nanoparticles (ZnO NPs) as multimodal drug delivery on human breast cancer MCF-7 cells.<br />Materials and Methods: In this in-vitro study, the impact of 5-FU and ZnO NPs in the single or combined forms was evaluated on cell viability, colony formation, apoptosis, p53 gene expression, and Bcl-2 signaling protein in MCF-7 breast cancer cell line using several techniques, such as MTT, clonogenic assay, flow cytometry, real-time quantitative polymerase chain reaction, and Western blot.<br />Results: In this study, 5-FU combined with ZnO NPs showed synergistic effects against MCF-7 within 48 hours. In addition, the combination of 5-FU and ZnO NPs at the respective concentrations of 1 µM and 45 µg/ml exhibited significant apoptosis (79.53%), p53 gene expression (3.6 folds), reduction of cell invasion (9.82%), and plating efficiency (5%), thereby leading to the significant reduction of cell viability (40±0.9%) and decreased Bcl-2 anti-apoptotic protein relative to untreated control cells. <br />Conclusion: According to the results, the synergistic effects of combined ZnO NPs and 5-FU on MCF-7 human breast cancer cells were exerted via Bcl-2 inhibition and the up-regulation of p53 expression. |
| first_indexed | 2024-12-14T00:33:17Z |
| format | Article |
| id | doaj.art-e9b66456badf4afbaac24fcb74967f4c |
| institution | Directory Open Access Journal |
| issn | 2322-3049 2322-5904 |
| language | English |
| last_indexed | 2024-12-14T00:33:17Z |
| publishDate | 2019-07-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Nanomedicine Journal |
| spelling | doaj.art-e9b66456badf4afbaac24fcb74967f4c2022-12-21T23:24:46ZengMashhad University of Medical SciencesNanomedicine Journal2322-30492322-59042019-07-016323224010.22038/nmj.2019.06.00001013111Effects of combined 5-Fluorouracil and ZnO NPs on human breast cancer MCF-7 Cells: P53 gene expression, Bcl-2 signaling pathway, and invasion activitySafoura Hoseinzadeh0Elham Raeisi1Yves Lemoigne2Esfandiar Heidarian3Clinical Biochemistry Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, IranDepartment of Medical Physics and Radiology, Shahrekord University of Medical Sciences, Shahrekord, IranInstitute for Medical Physics, Ambilly, FranceClinical Biochemistry Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, IranObjective(s): The significant contribution of nanoparticles to cancer treatment has attracted therapeutic attention. The present study aimed to evaluate the synergistic effects of 5-fluorouracil (5-FU) and zinc oxide nanoparticles (ZnO NPs) as multimodal drug delivery on human breast cancer MCF-7 cells.<br />Materials and Methods: In this in-vitro study, the impact of 5-FU and ZnO NPs in the single or combined forms was evaluated on cell viability, colony formation, apoptosis, p53 gene expression, and Bcl-2 signaling protein in MCF-7 breast cancer cell line using several techniques, such as MTT, clonogenic assay, flow cytometry, real-time quantitative polymerase chain reaction, and Western blot.<br />Results: In this study, 5-FU combined with ZnO NPs showed synergistic effects against MCF-7 within 48 hours. In addition, the combination of 5-FU and ZnO NPs at the respective concentrations of 1 µM and 45 µg/ml exhibited significant apoptosis (79.53%), p53 gene expression (3.6 folds), reduction of cell invasion (9.82%), and plating efficiency (5%), thereby leading to the significant reduction of cell viability (40±0.9%) and decreased Bcl-2 anti-apoptotic protein relative to untreated control cells. <br />Conclusion: According to the results, the synergistic effects of combined ZnO NPs and 5-FU on MCF-7 human breast cancer cells were exerted via Bcl-2 inhibition and the up-regulation of p53 expression.http://nmj.mums.ac.ir/article_13111_60382b380848d83580e1426dce38618b.pdfBcl-2p53 expressionZinc oxide naoparticles5-fluorouracil |
| spellingShingle | Safoura Hoseinzadeh Elham Raeisi Yves Lemoigne Esfandiar Heidarian Effects of combined 5-Fluorouracil and ZnO NPs on human breast cancer MCF-7 Cells: P53 gene expression, Bcl-2 signaling pathway, and invasion activity Nanomedicine Journal Bcl-2 p53 expression Zinc oxide naoparticles 5-fluorouracil |
| title | Effects of combined 5-Fluorouracil and ZnO NPs on human breast cancer MCF-7 Cells: P53 gene expression, Bcl-2 signaling pathway, and invasion activity |
| title_full | Effects of combined 5-Fluorouracil and ZnO NPs on human breast cancer MCF-7 Cells: P53 gene expression, Bcl-2 signaling pathway, and invasion activity |
| title_fullStr | Effects of combined 5-Fluorouracil and ZnO NPs on human breast cancer MCF-7 Cells: P53 gene expression, Bcl-2 signaling pathway, and invasion activity |
| title_full_unstemmed | Effects of combined 5-Fluorouracil and ZnO NPs on human breast cancer MCF-7 Cells: P53 gene expression, Bcl-2 signaling pathway, and invasion activity |
| title_short | Effects of combined 5-Fluorouracil and ZnO NPs on human breast cancer MCF-7 Cells: P53 gene expression, Bcl-2 signaling pathway, and invasion activity |
| title_sort | effects of combined 5 fluorouracil and zno nps on human breast cancer mcf 7 cells p53 gene expression bcl 2 signaling pathway and invasion activity |
| topic | Bcl-2 p53 expression Zinc oxide naoparticles 5-fluorouracil |
| url | http://nmj.mums.ac.ir/article_13111_60382b380848d83580e1426dce38618b.pdf |
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