Setting up a gene expression study for tissue cells by method of quantitative real-time PCR
Gene expression analysis by quantitative reverse transcription polymerase chain reaction (RT-qPCR) allows accurate and sensitive measurment of gene expression levels. However, a series of steps needs to be taken to ensure the relevance, accuracy, correct interpretation and repeatability of the RT-qP...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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University of Ljubljana Press (Založba Univerze v Ljubljani)
2012-07-01
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| Series: | Acta Agriculturae Slovenica |
| Subjects: | |
| Online Access: | https://journals.uni-lj.si/aas/article/view/14417 |
| _version_ | 1826532327222673408 |
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| author | Daliborka DUŠANIĆ Luka BOLHA Mojca NARAT Irena OVEN |
| author_facet | Daliborka DUŠANIĆ Luka BOLHA Mojca NARAT Irena OVEN |
| author_sort | Daliborka DUŠANIĆ |
| collection | DOAJ |
| description | Gene expression analysis by quantitative reverse transcription polymerase chain reaction (RT-qPCR) allows accurate and sensitive measurment of gene expression levels. However, a series of steps needs to be taken to ensure the relevance, accuracy, correct interpretation and repeatability of the RT-qPCR experiment. We describe here a simple experiment of determining relative gene expression for caspase 8 gene in chicken chondrocytes treated with an apoptosis inducing compound 5-fluorouracil. We use this example to point out some important guidelines in setting up a gene expression study in tissue cells, analyzing and interpreting the results and reporting on the findings. |
| first_indexed | 2024-03-08T23:24:18Z |
| format | Article |
| id | doaj.art-e9b737c20db5402389f9450dc9d117d4 |
| institution | Directory Open Access Journal |
| issn | 1854-1941 |
| language | English |
| last_indexed | 2025-03-14T01:49:28Z |
| publishDate | 2012-07-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Acta Agriculturae Slovenica |
| spelling | doaj.art-e9b737c20db5402389f9450dc9d117d42025-03-12T12:21:37ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19412012-07-011001192810.14720/aas.2012.100.1.1441720809Setting up a gene expression study for tissue cells by method of quantitative real-time PCRDaliborka DUŠANIĆ0Luka BOLHA1Mojca NARAT2Irena OVEN3Univ. of Ljubljana, Biotechnical Fac., Dept. of Animal Science, Groblje 3, SI-1230 Domžale, SloveniaUniv. of Ljubljana, Biotechnical Fac., Dept. of Animal Science, Groblje 3, SI-1230 Domžale, SloveniaUniv. of Ljubljana, Biotechnical Fac., Dept. of Animal Science, Groblje 3, SI-1230 Domžale, SloveniaUniv. of Ljubljana, Biotechnical Fac., Dept. of Animal Science, Groblje 3, SI-1230 Domžale, SloveniaGene expression analysis by quantitative reverse transcription polymerase chain reaction (RT-qPCR) allows accurate and sensitive measurment of gene expression levels. However, a series of steps needs to be taken to ensure the relevance, accuracy, correct interpretation and repeatability of the RT-qPCR experiment. We describe here a simple experiment of determining relative gene expression for caspase 8 gene in chicken chondrocytes treated with an apoptosis inducing compound 5-fluorouracil. We use this example to point out some important guidelines in setting up a gene expression study in tissue cells, analyzing and interpreting the results and reporting on the findings.https://journals.uni-lj.si/aas/article/view/14417molecular geneticsrt-qpcrgene expressiontissue cells |
| spellingShingle | Daliborka DUŠANIĆ Luka BOLHA Mojca NARAT Irena OVEN Setting up a gene expression study for tissue cells by method of quantitative real-time PCR Acta Agriculturae Slovenica molecular genetics rt-qpcr gene expression tissue cells |
| title | Setting up a gene expression study for tissue cells by method of quantitative real-time PCR |
| title_full | Setting up a gene expression study for tissue cells by method of quantitative real-time PCR |
| title_fullStr | Setting up a gene expression study for tissue cells by method of quantitative real-time PCR |
| title_full_unstemmed | Setting up a gene expression study for tissue cells by method of quantitative real-time PCR |
| title_short | Setting up a gene expression study for tissue cells by method of quantitative real-time PCR |
| title_sort | setting up a gene expression study for tissue cells by method of quantitative real time pcr |
| topic | molecular genetics rt-qpcr gene expression tissue cells |
| url | https://journals.uni-lj.si/aas/article/view/14417 |
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