Setting up a gene expression study for tissue cells by method of quantitative real-time PCR
Gene expression analysis by quantitative reverse transcription polymerase chain reaction (RT-qPCR) allows accurate and sensitive measurment of gene expression levels. However, a series of steps needs to be taken to ensure the relevance, accuracy, correct interpretation and repeatability of the RT-q...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
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University of Ljubljana Press (Založba Univerze v Ljubljani)
2012-07-01
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Series: | Acta Agriculturae Slovenica |
Subjects: | |
Online Access: | https://journals.uni-lj.si/aas/article/view/14417 |
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author | Daliborka DUŠANIĆ Luka BOLHA Mojca NARAT Irena OVEN |
author_facet | Daliborka DUŠANIĆ Luka BOLHA Mojca NARAT Irena OVEN |
author_sort | Daliborka DUŠANIĆ |
collection | DOAJ |
description |
Gene expression analysis by quantitative reverse transcription polymerase chain reaction (RT-qPCR) allows accurate and sensitive measurment of gene expression levels. However, a series of steps needs to be taken to ensure the relevance, accuracy, correct interpretation and repeatability of the RT-qPCR experiment. We describe here a simple experiment of determining relative gene expression for caspase 8 gene in chicken chondrocytes treated with an apoptosis inducing compound 5-fluorouracil. We use this example to point out some important guidelines in setting up a gene expression study in tissue cells, analyzing and interpreting the results and reporting on the findings.
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first_indexed | 2024-03-08T23:24:18Z |
format | Article |
id | doaj.art-e9b737c20db5402389f9450dc9d117d4 |
institution | Directory Open Access Journal |
issn | 1854-1941 |
language | English |
last_indexed | 2024-03-08T23:24:18Z |
publishDate | 2012-07-01 |
publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
record_format | Article |
series | Acta Agriculturae Slovenica |
spelling | doaj.art-e9b737c20db5402389f9450dc9d117d42023-12-14T22:14:24ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19412012-07-011001Setting up a gene expression study for tissue cells by method of quantitative real-time PCRDaliborka DUŠANIĆ0Luka BOLHA1Mojca NARAT2Irena OVEN3Univ. of Ljubljana, Biotechnical Fac., Dept. of Animal Science, Groblje 3, SI-1230 DomžaleUniv. of Ljubljana, Biotechnical Fac., Dept. of Animal Science, Groblje 3, SI-1230 DomžaleUniv. of Ljubljana, Biotechnical Fac., Dept. of Animal Science, Groblje 3, SI-1230 DomžaleUniv. of Ljubljana, Biotechnical Fac., Dept. of Animal Science, Groblje 3, SI-1230 Domžale Gene expression analysis by quantitative reverse transcription polymerase chain reaction (RT-qPCR) allows accurate and sensitive measurment of gene expression levels. However, a series of steps needs to be taken to ensure the relevance, accuracy, correct interpretation and repeatability of the RT-qPCR experiment. We describe here a simple experiment of determining relative gene expression for caspase 8 gene in chicken chondrocytes treated with an apoptosis inducing compound 5-fluorouracil. We use this example to point out some important guidelines in setting up a gene expression study in tissue cells, analyzing and interpreting the results and reporting on the findings. https://journals.uni-lj.si/aas/article/view/14417molecular geneticsRT-qPCRgene expressiontissue cells |
spellingShingle | Daliborka DUŠANIĆ Luka BOLHA Mojca NARAT Irena OVEN Setting up a gene expression study for tissue cells by method of quantitative real-time PCR Acta Agriculturae Slovenica molecular genetics RT-qPCR gene expression tissue cells |
title | Setting up a gene expression study for tissue cells by method of quantitative real-time PCR |
title_full | Setting up a gene expression study for tissue cells by method of quantitative real-time PCR |
title_fullStr | Setting up a gene expression study for tissue cells by method of quantitative real-time PCR |
title_full_unstemmed | Setting up a gene expression study for tissue cells by method of quantitative real-time PCR |
title_short | Setting up a gene expression study for tissue cells by method of quantitative real-time PCR |
title_sort | setting up a gene expression study for tissue cells by method of quantitative real time pcr |
topic | molecular genetics RT-qPCR gene expression tissue cells |
url | https://journals.uni-lj.si/aas/article/view/14417 |
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