Interior modification of Macrobrachium rosenbergii nodavirus-like particle enhances encapsulation of VP37-dsRNA against shrimp white spot syndrome infection
Abstract Background Application of a virus-like particle (VLP) as a nanocontainer to encapsulate double stranded (ds)RNA to control viral infection in shrimp aquaculture has been extensively reported. In this study, we aimed at improving VLP’s encapsulation efficiency which should lead to a superior...
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| Format: | Article |
| Language: | English |
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BMC
2024-03-01
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| Series: | BMC Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s12917-024-03936-w |
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| author | Itsares Muikham Orawan Thongsum Somkid Jaranathummakul Atthaboon Wathammawut Charoonroj Chotwiwatthanakun Pitchanee Jariyapong Wattana Weerachatyanukul |
| author_facet | Itsares Muikham Orawan Thongsum Somkid Jaranathummakul Atthaboon Wathammawut Charoonroj Chotwiwatthanakun Pitchanee Jariyapong Wattana Weerachatyanukul |
| author_sort | Itsares Muikham |
| collection | DOAJ |
| description | Abstract Background Application of a virus-like particle (VLP) as a nanocontainer to encapsulate double stranded (ds)RNA to control viral infection in shrimp aquaculture has been extensively reported. In this study, we aimed at improving VLP’s encapsulation efficiency which should lead to a superior fighting weapon with disastrous viruses. Results We constructed 2 variants of chimeric Macrobrachium rosenbergii nodavirus (MrNV)-like particles (V1- and V2-MrN-VLPs) and tested their efficiency to encapsulate VP37 double stranded RNA as well as WSSV protection in P. vannamei. Two types of short peptides, RNA-binding domain (RBD) and deca-arginine (10R) were successfully engineered into the interior surface of VLP, the site where the contact with VP37-dsRNA occurs. TEM and dynamic light scattering (DLS) analyses revealed that the chimeric VLPs remained their assembling property to be an icosahedral symmetric particle with a diameter of about 30 nm, similar to the original MrN-VLP particle. The superior encapsulation efficiency of VP37-dsRNA into V2-MrN-VLP was achieved, which was slightly better than that of V1-MrN-VLP but far better (1.4-fold) than its parental V0-MrN-VLP which the mole ratio of 7.5–10.5 for all VLP variants. The protection effect against challenging WSSV (as gauged from the level of VP37 gene and the remaining viral copy number in shrimp) was significantly improved in both V1- and V2-MrN-VLP compared with an original V0-MrN-VLP template. Conclusion MrN-VLP (V0-) were re-engineered interiorly with RBD (V1-) and 10R (V2-) peptides which had an improved VP37-dsRNA encapsulation capability. The protection effect against WSSV infection through shrimp administration with dsRNA + V1-/V2-MrN VLPs was experimentally evident. |
| first_indexed | 2024-04-25T01:06:34Z |
| format | Article |
| id | doaj.art-e9b811863f5845499c6871ca37aee95c |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-04-25T01:06:34Z |
| publishDate | 2024-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-e9b811863f5845499c6871ca37aee95c2024-03-10T12:15:06ZengBMCBMC Veterinary Research1746-61482024-03-0120111110.1186/s12917-024-03936-wInterior modification of Macrobrachium rosenbergii nodavirus-like particle enhances encapsulation of VP37-dsRNA against shrimp white spot syndrome infectionItsares Muikham0Orawan Thongsum1Somkid Jaranathummakul2Atthaboon Wathammawut3Charoonroj Chotwiwatthanakun4Pitchanee Jariyapong5Wattana Weerachatyanukul6Department of Anatomy, Faculty of Science, Mahidol UniversityDepartment of Anatomy, Faculty of Science, Mahidol UniversityDepartment of Anatomy, Faculty of Science, Mahidol UniversityDepartment of Anatomy, Faculty of Medicine, Srinakharinwirot UniversityFaculty of Science, Mahidol UniversityDepartment of Medical Science, School of Medicine, Walailak UniversityDepartment of Anatomy, Faculty of Science, Mahidol UniversityAbstract Background Application of a virus-like particle (VLP) as a nanocontainer to encapsulate double stranded (ds)RNA to control viral infection in shrimp aquaculture has been extensively reported. In this study, we aimed at improving VLP’s encapsulation efficiency which should lead to a superior fighting weapon with disastrous viruses. Results We constructed 2 variants of chimeric Macrobrachium rosenbergii nodavirus (MrNV)-like particles (V1- and V2-MrN-VLPs) and tested their efficiency to encapsulate VP37 double stranded RNA as well as WSSV protection in P. vannamei. Two types of short peptides, RNA-binding domain (RBD) and deca-arginine (10R) were successfully engineered into the interior surface of VLP, the site where the contact with VP37-dsRNA occurs. TEM and dynamic light scattering (DLS) analyses revealed that the chimeric VLPs remained their assembling property to be an icosahedral symmetric particle with a diameter of about 30 nm, similar to the original MrN-VLP particle. The superior encapsulation efficiency of VP37-dsRNA into V2-MrN-VLP was achieved, which was slightly better than that of V1-MrN-VLP but far better (1.4-fold) than its parental V0-MrN-VLP which the mole ratio of 7.5–10.5 for all VLP variants. The protection effect against challenging WSSV (as gauged from the level of VP37 gene and the remaining viral copy number in shrimp) was significantly improved in both V1- and V2-MrN-VLP compared with an original V0-MrN-VLP template. Conclusion MrN-VLP (V0-) were re-engineered interiorly with RBD (V1-) and 10R (V2-) peptides which had an improved VP37-dsRNA encapsulation capability. The protection effect against WSSV infection through shrimp administration with dsRNA + V1-/V2-MrN VLPs was experimentally evident.https://doi.org/10.1186/s12917-024-03936-wMrN Virus-like particleWSSVdsRNAVP37 |
| spellingShingle | Itsares Muikham Orawan Thongsum Somkid Jaranathummakul Atthaboon Wathammawut Charoonroj Chotwiwatthanakun Pitchanee Jariyapong Wattana Weerachatyanukul Interior modification of Macrobrachium rosenbergii nodavirus-like particle enhances encapsulation of VP37-dsRNA against shrimp white spot syndrome infection BMC Veterinary Research MrN Virus-like particle WSSV dsRNA VP37 |
| title | Interior modification of Macrobrachium rosenbergii nodavirus-like particle enhances encapsulation of VP37-dsRNA against shrimp white spot syndrome infection |
| title_full | Interior modification of Macrobrachium rosenbergii nodavirus-like particle enhances encapsulation of VP37-dsRNA against shrimp white spot syndrome infection |
| title_fullStr | Interior modification of Macrobrachium rosenbergii nodavirus-like particle enhances encapsulation of VP37-dsRNA against shrimp white spot syndrome infection |
| title_full_unstemmed | Interior modification of Macrobrachium rosenbergii nodavirus-like particle enhances encapsulation of VP37-dsRNA against shrimp white spot syndrome infection |
| title_short | Interior modification of Macrobrachium rosenbergii nodavirus-like particle enhances encapsulation of VP37-dsRNA against shrimp white spot syndrome infection |
| title_sort | interior modification of macrobrachium rosenbergii nodavirus like particle enhances encapsulation of vp37 dsrna against shrimp white spot syndrome infection |
| topic | MrN Virus-like particle WSSV dsRNA VP37 |
| url | https://doi.org/10.1186/s12917-024-03936-w |
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