Variations in Fibrinogen-like 1 (<i>FGL1</i>) Gene Locus as a Genetic Marker Related to Fat Deposition Based on Pig Model and Liver RNA-Seq Data
The goal of this study was to evaluate the effects of mutations in the <i>FGL1</i> gene associated with pig productive traits to enrich the genetic marker pool for further selection and to support the studies on <i>FGL1</i> in the context of the fat deposition (FD) process. T...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-08-01
|
| Series: | Genes |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4425/13/8/1419 |
| _version_ | 1797432196662820864 |
|---|---|
| author | Katarzyna Piórkowska Kacper Żukowski Katarzyna Ropka-Molik Mirosław Tyra |
| author_facet | Katarzyna Piórkowska Kacper Żukowski Katarzyna Ropka-Molik Mirosław Tyra |
| author_sort | Katarzyna Piórkowska |
| collection | DOAJ |
| description | The goal of this study was to evaluate the effects of mutations in the <i>FGL1</i> gene associated with pig productive traits to enrich the genetic marker pool for further selection and to support the studies on <i>FGL1</i> in the context of the fat deposition (FD) process. The variant calling and χ<sup>2</sup> analyses of liver RNA-seq data were used to indicate genetic markers. <i>FGL1</i> mutations were genotyped in the Złotnicka White (n = 72), Polish Large White (n = 208), Duroc (n = 72), Polish Landrace (PL) (n = 292), and Puławska (n = 178) pig breeds. An association study was performed using a general linear model (GLM) implemented in SAS<sup>®</sup> software. More than 50 crucial mutations were identified in the <i>FGL1</i> gene. The association study showed a significant effect of the <i>FGL1</i> on intramuscular fat (IMF), loin eye area, backfat thickness at the lumbar, ham mass (<i>p</i> = 0.0374), meat percentage (<i>p</i> = 0.0205), and loin fat (<i>p</i> = 0.0003). Alternate homozygotes and heterozygotes were found in the PL and Duroc, confirming the selective potential for these populations. Our study supports the theory that liver <i>FGL1</i> is involved in the FD process. Moreover, since fat is the major determinant of flavor development in meat, the <i>FGL1</i> rs340465447_A allele can be used as a target in pig selection focused on elevated fat levels. |
| first_indexed | 2024-03-09T09:56:49Z |
| format | Article |
| id | doaj.art-e9b8e6ac1d684cf0b39c68026c2b3cbe |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T09:56:49Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-e9b8e6ac1d684cf0b39c68026c2b3cbe2023-12-01T23:44:16ZengMDPI AGGenes2073-44252022-08-01138141910.3390/genes13081419Variations in Fibrinogen-like 1 (<i>FGL1</i>) Gene Locus as a Genetic Marker Related to Fat Deposition Based on Pig Model and Liver RNA-Seq DataKatarzyna Piórkowska0Kacper Żukowski1Katarzyna Ropka-Molik2Mirosław Tyra3Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, PolandDepartment of Cattle Breeding, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, PolandDepartment of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, PolandDepartment of Pig Breeding, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, PolandThe goal of this study was to evaluate the effects of mutations in the <i>FGL1</i> gene associated with pig productive traits to enrich the genetic marker pool for further selection and to support the studies on <i>FGL1</i> in the context of the fat deposition (FD) process. The variant calling and χ<sup>2</sup> analyses of liver RNA-seq data were used to indicate genetic markers. <i>FGL1</i> mutations were genotyped in the Złotnicka White (n = 72), Polish Large White (n = 208), Duroc (n = 72), Polish Landrace (PL) (n = 292), and Puławska (n = 178) pig breeds. An association study was performed using a general linear model (GLM) implemented in SAS<sup>®</sup> software. More than 50 crucial mutations were identified in the <i>FGL1</i> gene. The association study showed a significant effect of the <i>FGL1</i> on intramuscular fat (IMF), loin eye area, backfat thickness at the lumbar, ham mass (<i>p</i> = 0.0374), meat percentage (<i>p</i> = 0.0205), and loin fat (<i>p</i> = 0.0003). Alternate homozygotes and heterozygotes were found in the PL and Duroc, confirming the selective potential for these populations. Our study supports the theory that liver <i>FGL1</i> is involved in the FD process. Moreover, since fat is the major determinant of flavor development in meat, the <i>FGL1</i> rs340465447_A allele can be used as a target in pig selection focused on elevated fat levels.https://www.mdpi.com/2073-4425/13/8/1419fibrinogen-like 1<i>FGL1</i>pigfat depositionselective markers |
| spellingShingle | Katarzyna Piórkowska Kacper Żukowski Katarzyna Ropka-Molik Mirosław Tyra Variations in Fibrinogen-like 1 (<i>FGL1</i>) Gene Locus as a Genetic Marker Related to Fat Deposition Based on Pig Model and Liver RNA-Seq Data Genes fibrinogen-like 1 <i>FGL1</i> pig fat deposition selective markers |
| title | Variations in Fibrinogen-like 1 (<i>FGL1</i>) Gene Locus as a Genetic Marker Related to Fat Deposition Based on Pig Model and Liver RNA-Seq Data |
| title_full | Variations in Fibrinogen-like 1 (<i>FGL1</i>) Gene Locus as a Genetic Marker Related to Fat Deposition Based on Pig Model and Liver RNA-Seq Data |
| title_fullStr | Variations in Fibrinogen-like 1 (<i>FGL1</i>) Gene Locus as a Genetic Marker Related to Fat Deposition Based on Pig Model and Liver RNA-Seq Data |
| title_full_unstemmed | Variations in Fibrinogen-like 1 (<i>FGL1</i>) Gene Locus as a Genetic Marker Related to Fat Deposition Based on Pig Model and Liver RNA-Seq Data |
| title_short | Variations in Fibrinogen-like 1 (<i>FGL1</i>) Gene Locus as a Genetic Marker Related to Fat Deposition Based on Pig Model and Liver RNA-Seq Data |
| title_sort | variations in fibrinogen like 1 i fgl1 i gene locus as a genetic marker related to fat deposition based on pig model and liver rna seq data |
| topic | fibrinogen-like 1 <i>FGL1</i> pig fat deposition selective markers |
| url | https://www.mdpi.com/2073-4425/13/8/1419 |
| work_keys_str_mv | AT katarzynapiorkowska variationsinfibrinogenlike1ifgl1igenelocusasageneticmarkerrelatedtofatdepositionbasedonpigmodelandliverrnaseqdata AT kacperzukowski variationsinfibrinogenlike1ifgl1igenelocusasageneticmarkerrelatedtofatdepositionbasedonpigmodelandliverrnaseqdata AT katarzynaropkamolik variationsinfibrinogenlike1ifgl1igenelocusasageneticmarkerrelatedtofatdepositionbasedonpigmodelandliverrnaseqdata AT mirosławtyra variationsinfibrinogenlike1ifgl1igenelocusasageneticmarkerrelatedtofatdepositionbasedonpigmodelandliverrnaseqdata |