| Summary: | The basic leucine zipper (bZIP) transcription factors constitute the most widely distributed and conserved eukaryotic family. They play crucial roles in plant growth, development, and responses to both biotic and abiotic stresses, exerting strong regulatory control over the expression of downstream genes. In this study, a genome-wide characterization of the CebZIP transcription factor family was conducted using bioinformatic analysis. Various aspects, including physicochemical properties, phylogenetics, conserved structural domains, gene structures, chromosomal distribution, gene covariance relationships, promoter <i>cis</i>-acting elements, and gene expression patterns, were thoroughly analyzed. A total of 70 <i>CebZIP</i> genes were identified from the <i>C. ensifolium</i> genome, and they were randomly distributed across 18 chromosomes. The phylogenetic tree clustered them into 11 subfamilies, each exhibiting complex gene structures and conserved motifs arranged in a specific order. Nineteen pairs of duplicated genes were identified among the 70 <i>CebZIP</i> genes, with sixteen pairs affected by purifying selection. <i>Cis</i>-acting elements analysis revealed a plethora of regulatory elements associated with stress response, plant hormones, and plant growth and development. Transcriptome and qRT-PCR results demonstrated that the expression of <i>CebZIP</i> genes was universally up-regulated under low temperature conditions. However, the expression patterns varied among different members. This study provides theoretical references for identifying key <i>bZIP</i> genes in <i>C. ensifolium</i> that confer resistance to low-temperature stress, and lays the groundwork for further research into their broader biological functions.
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