Characterization of silk genes in Ephestia kuehniella and Galleria mellonella revealed duplication of sericin genes and highly divergent sequences encoding fibroin heavy chains
Silk is a secretory product of numerous arthropods with remarkable mechanical properties. In this work, we present the complete sequences of the putative major silk proteins of E. kuehniella and compare them with those of G. mellonella, which belongs to the same moth family Pyralidae. To identify th...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Molecular Biosciences |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2022.1023381/full |
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| author | Bulah Chia-hsiang Wu Bulah Chia-hsiang Wu Ivo Sauman Ivo Sauman Houda Ouns Maaroufi Houda Ouns Maaroufi Anna Zaloudikova Martina Zurovcova Barbara Kludkiewicz Miluse Hradilova Michal Zurovec Michal Zurovec |
| author_facet | Bulah Chia-hsiang Wu Bulah Chia-hsiang Wu Ivo Sauman Ivo Sauman Houda Ouns Maaroufi Houda Ouns Maaroufi Anna Zaloudikova Martina Zurovcova Barbara Kludkiewicz Miluse Hradilova Michal Zurovec Michal Zurovec |
| author_sort | Bulah Chia-hsiang Wu |
| collection | DOAJ |
| description | Silk is a secretory product of numerous arthropods with remarkable mechanical properties. In this work, we present the complete sequences of the putative major silk proteins of E. kuehniella and compare them with those of G. mellonella, which belongs to the same moth family Pyralidae. To identify the silk genes of both species, we combined proteomic analysis of cocoon silk with a homology search in transcriptomes and genomic sequences to complement the information on both species. We analyzed structure of the candidate genes obtained, their expression specificity and their evolutionary relationships. We demonstrate that the silks of E. kuehniella and G. mellonella differ in their hydrophobicity and that the silk of E. kuehniella is highly hygroscopic. In our experiments, we show that the number of genes encoding sericins is higher in G. mellonella than in E. kuehniella. By analyzing the synteny of the chromosomal segment encoding sericin genes in both moth species, we found that the region encoding sericins is duplicated in G. mellonella. Finally, we present the complete primary structures of nine fibH genes and proteins from both families of the suborder Pyraloidea and discuss their specific and conserved features. This study provides a foundation for future research on the evolution of silk proteins and lays the groundwork for future detailed functional studies. |
| first_indexed | 2024-04-12T06:25:26Z |
| format | Article |
| id | doaj.art-e9c2ebf179ef43ce8d508af45bb8f8a2 |
| institution | Directory Open Access Journal |
| issn | 2296-889X |
| language | English |
| last_indexed | 2024-04-12T06:25:26Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-e9c2ebf179ef43ce8d508af45bb8f8a22022-12-22T03:44:11ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2022-11-01910.3389/fmolb.2022.10233811023381Characterization of silk genes in Ephestia kuehniella and Galleria mellonella revealed duplication of sericin genes and highly divergent sequences encoding fibroin heavy chainsBulah Chia-hsiang Wu0Bulah Chia-hsiang Wu1Ivo Sauman2Ivo Sauman3Houda Ouns Maaroufi4Houda Ouns Maaroufi5Anna Zaloudikova6Martina Zurovcova7Barbara Kludkiewicz8Miluse Hradilova9Michal Zurovec10Michal Zurovec11Biology Centre of the Czech Academy of Sciences, Institute of Entomology, Ceske Budejovice, CzechiaFaculty of Science, University of South Bohemia, Ceske Budejovice, CzechiaBiology Centre of the Czech Academy of Sciences, Institute of Entomology, Ceske Budejovice, CzechiaFaculty of Science, University of South Bohemia, Ceske Budejovice, CzechiaBiology Centre of the Czech Academy of Sciences, Institute of Entomology, Ceske Budejovice, CzechiaFaculty of Science, University of South Bohemia, Ceske Budejovice, CzechiaBiology Centre of the Czech Academy of Sciences, Institute of Entomology, Ceske Budejovice, CzechiaBiology Centre of the Czech Academy of Sciences, Institute of Entomology, Ceske Budejovice, CzechiaBiology Centre of the Czech Academy of Sciences, Institute of Entomology, Ceske Budejovice, CzechiaInstitute of Molecular Genetics, Academy of Sciences of the Czech Republic, Praha, CzechiaBiology Centre of the Czech Academy of Sciences, Institute of Entomology, Ceske Budejovice, CzechiaFaculty of Science, University of South Bohemia, Ceske Budejovice, CzechiaSilk is a secretory product of numerous arthropods with remarkable mechanical properties. In this work, we present the complete sequences of the putative major silk proteins of E. kuehniella and compare them with those of G. mellonella, which belongs to the same moth family Pyralidae. To identify the silk genes of both species, we combined proteomic analysis of cocoon silk with a homology search in transcriptomes and genomic sequences to complement the information on both species. We analyzed structure of the candidate genes obtained, their expression specificity and their evolutionary relationships. We demonstrate that the silks of E. kuehniella and G. mellonella differ in their hydrophobicity and that the silk of E. kuehniella is highly hygroscopic. In our experiments, we show that the number of genes encoding sericins is higher in G. mellonella than in E. kuehniella. By analyzing the synteny of the chromosomal segment encoding sericin genes in both moth species, we found that the region encoding sericins is duplicated in G. mellonella. Finally, we present the complete primary structures of nine fibH genes and proteins from both families of the suborder Pyraloidea and discuss their specific and conserved features. This study provides a foundation for future research on the evolution of silk proteins and lays the groundwork for future detailed functional studies.https://www.frontiersin.org/articles/10.3389/fmolb.2022.1023381/fullsyntenymucinmediterranean flour mothwax mothpyralidaecrambidae |
| spellingShingle | Bulah Chia-hsiang Wu Bulah Chia-hsiang Wu Ivo Sauman Ivo Sauman Houda Ouns Maaroufi Houda Ouns Maaroufi Anna Zaloudikova Martina Zurovcova Barbara Kludkiewicz Miluse Hradilova Michal Zurovec Michal Zurovec Characterization of silk genes in Ephestia kuehniella and Galleria mellonella revealed duplication of sericin genes and highly divergent sequences encoding fibroin heavy chains Frontiers in Molecular Biosciences synteny mucin mediterranean flour moth wax moth pyralidae crambidae |
| title | Characterization of silk genes in Ephestia kuehniella and Galleria mellonella revealed duplication of sericin genes and highly divergent sequences encoding fibroin heavy chains |
| title_full | Characterization of silk genes in Ephestia kuehniella and Galleria mellonella revealed duplication of sericin genes and highly divergent sequences encoding fibroin heavy chains |
| title_fullStr | Characterization of silk genes in Ephestia kuehniella and Galleria mellonella revealed duplication of sericin genes and highly divergent sequences encoding fibroin heavy chains |
| title_full_unstemmed | Characterization of silk genes in Ephestia kuehniella and Galleria mellonella revealed duplication of sericin genes and highly divergent sequences encoding fibroin heavy chains |
| title_short | Characterization of silk genes in Ephestia kuehniella and Galleria mellonella revealed duplication of sericin genes and highly divergent sequences encoding fibroin heavy chains |
| title_sort | characterization of silk genes in ephestia kuehniella and galleria mellonella revealed duplication of sericin genes and highly divergent sequences encoding fibroin heavy chains |
| topic | synteny mucin mediterranean flour moth wax moth pyralidae crambidae |
| url | https://www.frontiersin.org/articles/10.3389/fmolb.2022.1023381/full |
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