Evolution of erythrocyte morphology in amphibians (Amphibia: Anura)
ABSTRACT We compared the morphology of the erythrocytes of five anurans, two toad species - Bufo gargarizans (Cantor, 1842) and Duttaphrynus melanostictus (Schneider, 1799) and three frog species - Fejervarya limnocharis (Gravenhorst, 1829), Microhyla ornata (Duméril & Bibron, 1841), and Rana zh...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Zoologia
2015-10-01
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| Series: | Zoologia (Curitiba) |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702015000500360&lng=en&tlng=en |
| _version_ | 1797965016646811648 |
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| author | Jie Wei Yan-Yan Li Li Wei Guo-Hua Ding Xiao-Li Fan Zhi-Hua Lin |
| author_facet | Jie Wei Yan-Yan Li Li Wei Guo-Hua Ding Xiao-Li Fan Zhi-Hua Lin |
| author_sort | Jie Wei |
| collection | DOAJ |
| description | ABSTRACT We compared the morphology of the erythrocytes of five anurans, two toad species - Bufo gargarizans (Cantor, 1842) and Duttaphrynus melanostictus (Schneider, 1799) and three frog species - Fejervarya limnocharis (Gravenhorst, 1829), Microhyla ornata (Duméril & Bibron, 1841), and Rana zhenhaiensis (Ye, Fei & Matsui, 1995). We then reconstructed the ancestral state of erythrocyte size (ES) and nuclear size (NS) in amphibians based on a molecular tree. Nine morphological traits of erythrocytes were all significantly different among the five species. The results of principal component analysis showed that the first component (49.1% of variance explained) had a high positive loading for erythrocyte length, nuclear length, NS and ratio of erythrocyte length/erythrocyte width; the second axis (28.5% of variance explained) mainly represented erythrocyte width and ES. Phylogenetic generalized least squares analysis showed that the relationship between NS and ES was not affected by phylogenetic relationships although there was a significant linear relationship between these two variables. These results suggested that (1) the nine morphological traits of erythrocytes in the five anuran species were species-specific; (2) in amphibians, larger erythrocytes generally had larger nuclei. |
| first_indexed | 2024-04-11T01:52:33Z |
| format | Article |
| id | doaj.art-e9c53be373bb4bb988faf53264416309 |
| institution | Directory Open Access Journal |
| issn | 1984-4689 |
| language | English |
| last_indexed | 2024-04-11T01:52:33Z |
| publishDate | 2015-10-01 |
| publisher | Sociedade Brasileira de Zoologia |
| record_format | Article |
| series | Zoologia (Curitiba) |
| spelling | doaj.art-e9c53be373bb4bb988faf532644163092023-01-03T06:02:53ZengSociedade Brasileira de ZoologiaZoologia (Curitiba)1984-46892015-10-0132536037010.1590/S1984-46702015000500005S1984-46702015000500360Evolution of erythrocyte morphology in amphibians (Amphibia: Anura)Jie WeiYan-Yan LiLi WeiGuo-Hua DingXiao-Li FanZhi-Hua LinABSTRACT We compared the morphology of the erythrocytes of five anurans, two toad species - Bufo gargarizans (Cantor, 1842) and Duttaphrynus melanostictus (Schneider, 1799) and three frog species - Fejervarya limnocharis (Gravenhorst, 1829), Microhyla ornata (Duméril & Bibron, 1841), and Rana zhenhaiensis (Ye, Fei & Matsui, 1995). We then reconstructed the ancestral state of erythrocyte size (ES) and nuclear size (NS) in amphibians based on a molecular tree. Nine morphological traits of erythrocytes were all significantly different among the five species. The results of principal component analysis showed that the first component (49.1% of variance explained) had a high positive loading for erythrocyte length, nuclear length, NS and ratio of erythrocyte length/erythrocyte width; the second axis (28.5% of variance explained) mainly represented erythrocyte width and ES. Phylogenetic generalized least squares analysis showed that the relationship between NS and ES was not affected by phylogenetic relationships although there was a significant linear relationship between these two variables. These results suggested that (1) the nine morphological traits of erythrocytes in the five anuran species were species-specific; (2) in amphibians, larger erythrocytes generally had larger nuclei.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702015000500360&lng=en&tlng=enAmphibiaancestral state reconstructionerythrocyte sizemorphological comparisonnucleus size. |
| spellingShingle | Jie Wei Yan-Yan Li Li Wei Guo-Hua Ding Xiao-Li Fan Zhi-Hua Lin Evolution of erythrocyte morphology in amphibians (Amphibia: Anura) Zoologia (Curitiba) Amphibia ancestral state reconstruction erythrocyte size morphological comparison nucleus size. |
| title | Evolution of erythrocyte morphology in amphibians (Amphibia: Anura) |
| title_full | Evolution of erythrocyte morphology in amphibians (Amphibia: Anura) |
| title_fullStr | Evolution of erythrocyte morphology in amphibians (Amphibia: Anura) |
| title_full_unstemmed | Evolution of erythrocyte morphology in amphibians (Amphibia: Anura) |
| title_short | Evolution of erythrocyte morphology in amphibians (Amphibia: Anura) |
| title_sort | evolution of erythrocyte morphology in amphibians amphibia anura |
| topic | Amphibia ancestral state reconstruction erythrocyte size morphological comparison nucleus size. |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702015000500360&lng=en&tlng=en |
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