Optimization of Fermentation Process for Water-soluble Polysaccharides and Antioxidant Activity of Polygonatum odoratum Fermentated by Bacillus subtilis LY-05
To investigate the optimal process conditions for the production of water-soluble polysaccharides from Polygonatum odoratum by aprobiotics and compare the change of antioxidant activities of polysaccharides fermented and unfermented. In this study, Bacillus subtilis LY-05 was used as fermentation st...
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2022-02-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021060232 |
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| author | Yujie LIU Liting DONG Can LUO Shaoshu CHEN Fengteng XIA Zheng WANG |
| author_facet | Yujie LIU Liting DONG Can LUO Shaoshu CHEN Fengteng XIA Zheng WANG |
| author_sort | Yujie LIU |
| collection | DOAJ |
| description | To investigate the optimal process conditions for the production of water-soluble polysaccharides from Polygonatum odoratum by aprobiotics and compare the change of antioxidant activities of polysaccharides fermented and unfermented. In this study, Bacillus subtilis LY-05 was used as fermentation strain, and increasing rate of water-soluble polysaccharides was index. Seven factors including Polygonatum odoratum addition, nitrogen source, inorganic salt, inoculation amount, temperature, stirring speed and fermentation time were studied with the increasing rate of water-soluble polysaccharides. On the basis of single-factor experiment, the fermentation conditions were optimized with response surface method; and evaluating the changes of the DPPH·scavenging rate, ABTS+·scavenging rate, OH· scavenging rate and total reducing power in the antioxidant activity of Polygonatum odoratum polysaccharides fermented and unfermented. The results showed that optimal fermentation condition was as follows: Polygonatum odoratum addition 4%, yeast extract powder 3%, NaCl 1%, inoculation amount 3%, fermention temperature 35 ℃, stirrig speed 160 r/min, fermentation time 48 h. Under these conditions, the polysaccharide content reached 7.83 mg/mL, which was 71.78% higher than that before fermentation(4.56 mg/mL). The antioxidant experiment showed that the DPPH·, ABTS+·, OH· scavenging rate and total reducing power of the fermented polysaccharide increased at certain concentrations and showed polysaccharide concentration dependence. The semi-inhibitory concentration (IC50) of the fermented polysaccharide POP2 on ABTS+·radical scavenging was 2.21 mg/mL, and the semi-inhibitory concentration on OH·radical scavenging was 0.49 mg/mL. These results implied that fermentation with Bacillus subtilis could significantly improve the extraction efficiency of Polygonatum odoratum polysaccharides, and polysaccharides in fermentated Polygonatum odoratum had better antioxidant capacity. |
| first_indexed | 2024-04-13T09:12:46Z |
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| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-13T09:12:46Z |
| publishDate | 2022-02-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-e9c7587da270411db650026f36d4195b2022-12-22T02:52:51ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-02-0143321222110.13386/j.issn1002-0306.20210602322021060232-3Optimization of Fermentation Process for Water-soluble Polysaccharides and Antioxidant Activity of Polygonatum odoratum Fermentated by Bacillus subtilis LY-05Yujie LIU0Liting DONG1Can LUO2Shaoshu CHEN3Fengteng XIA4Zheng WANG5College of Biological Science and Technology, Hunan Agricultural University, Changsha 410000, ChinaHunan Imagination Agricultural Science and Technology, Zhangjiajie 427000, ChinaCollege of Biological Science and Technology, Hunan Agricultural University, Changsha 410000, ChinaCollege of Biological Science and Technology, Hunan Agricultural University, Changsha 410000, ChinaCollege of Biological Science and Technology, Hunan Agricultural University, Changsha 410000, ChinaCollege of Biological Science and Technology, Hunan Agricultural University, Changsha 410000, ChinaTo investigate the optimal process conditions for the production of water-soluble polysaccharides from Polygonatum odoratum by aprobiotics and compare the change of antioxidant activities of polysaccharides fermented and unfermented. In this study, Bacillus subtilis LY-05 was used as fermentation strain, and increasing rate of water-soluble polysaccharides was index. Seven factors including Polygonatum odoratum addition, nitrogen source, inorganic salt, inoculation amount, temperature, stirring speed and fermentation time were studied with the increasing rate of water-soluble polysaccharides. On the basis of single-factor experiment, the fermentation conditions were optimized with response surface method; and evaluating the changes of the DPPH·scavenging rate, ABTS+·scavenging rate, OH· scavenging rate and total reducing power in the antioxidant activity of Polygonatum odoratum polysaccharides fermented and unfermented. The results showed that optimal fermentation condition was as follows: Polygonatum odoratum addition 4%, yeast extract powder 3%, NaCl 1%, inoculation amount 3%, fermention temperature 35 ℃, stirrig speed 160 r/min, fermentation time 48 h. Under these conditions, the polysaccharide content reached 7.83 mg/mL, which was 71.78% higher than that before fermentation(4.56 mg/mL). The antioxidant experiment showed that the DPPH·, ABTS+·, OH· scavenging rate and total reducing power of the fermented polysaccharide increased at certain concentrations and showed polysaccharide concentration dependence. The semi-inhibitory concentration (IC50) of the fermented polysaccharide POP2 on ABTS+·radical scavenging was 2.21 mg/mL, and the semi-inhibitory concentration on OH·radical scavenging was 0.49 mg/mL. These results implied that fermentation with Bacillus subtilis could significantly improve the extraction efficiency of Polygonatum odoratum polysaccharides, and polysaccharides in fermentated Polygonatum odoratum had better antioxidant capacity.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021060232polygonatum odoratum polysaccharidebacillus subtilis ly-05fermentation processwater-soluble polysaccharideantioxidant activity |
| spellingShingle | Yujie LIU Liting DONG Can LUO Shaoshu CHEN Fengteng XIA Zheng WANG Optimization of Fermentation Process for Water-soluble Polysaccharides and Antioxidant Activity of Polygonatum odoratum Fermentated by Bacillus subtilis LY-05 Shipin gongye ke-ji polygonatum odoratum polysaccharide bacillus subtilis ly-05 fermentation process water-soluble polysaccharide antioxidant activity |
| title | Optimization of Fermentation Process for Water-soluble Polysaccharides and Antioxidant Activity of Polygonatum odoratum Fermentated by Bacillus subtilis LY-05 |
| title_full | Optimization of Fermentation Process for Water-soluble Polysaccharides and Antioxidant Activity of Polygonatum odoratum Fermentated by Bacillus subtilis LY-05 |
| title_fullStr | Optimization of Fermentation Process for Water-soluble Polysaccharides and Antioxidant Activity of Polygonatum odoratum Fermentated by Bacillus subtilis LY-05 |
| title_full_unstemmed | Optimization of Fermentation Process for Water-soluble Polysaccharides and Antioxidant Activity of Polygonatum odoratum Fermentated by Bacillus subtilis LY-05 |
| title_short | Optimization of Fermentation Process for Water-soluble Polysaccharides and Antioxidant Activity of Polygonatum odoratum Fermentated by Bacillus subtilis LY-05 |
| title_sort | optimization of fermentation process for water soluble polysaccharides and antioxidant activity of polygonatum odoratum fermentated by bacillus subtilis ly 05 |
| topic | polygonatum odoratum polysaccharide bacillus subtilis ly-05 fermentation process water-soluble polysaccharide antioxidant activity |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021060232 |
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