Cj1411c encodes for a cytochrome P450 involved in Campylobacter jejuni 81-176 pathogenicity.
Cytochrome P450s are b-heme-containing enzymes that are able to introduce oxygen atoms into a wide variety of organic substrates. They are extremely widespread in nature having diverse functions at both biochemical and physiological level. The genome of C. jejuni 81-176 encodes a single cytochrome P...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3784454?pdf=render |
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| author | Luis A J Alvarez Billy Bourke Gratiela Pircalabioru Atanas Y Georgiev Ulla G Knaus Simon Daff Nicolae Corcionivoschi |
| author_facet | Luis A J Alvarez Billy Bourke Gratiela Pircalabioru Atanas Y Georgiev Ulla G Knaus Simon Daff Nicolae Corcionivoschi |
| author_sort | Luis A J Alvarez |
| collection | DOAJ |
| description | Cytochrome P450s are b-heme-containing enzymes that are able to introduce oxygen atoms into a wide variety of organic substrates. They are extremely widespread in nature having diverse functions at both biochemical and physiological level. The genome of C. jejuni 81-176 encodes a single cytochrome P450 (Cj1411c) that has no close homologues. Cj1411c is unusual in its genomic location within a cluster involved in the biosynthesis of outer surface structures. Here we show that E. coli expressed and affinity-purified C. jejuni cytochrome P450 is lipophilic, containing one equivalent Cys-ligated heme. Immunoblotting confirmed the association of cytochrome P450 with membrane fractions. A Cj1411c deletion mutant had significantly reduced ability to infect human cells and was less able to survive following exposure to human serum when compared to the wild type strain. Phenotypically following staining with Alcian blue, we show that a Cj1411c deletion mutant produces significantly less capsular polysaccharide. This study describes the first known membrane-bound bacterial cytochrome P450 and its involvement in Campylobacter virulence. |
| first_indexed | 2024-12-21T13:20:54Z |
| format | Article |
| id | doaj.art-e9c761b1d6374d5b84c36d410f4e74a2 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T13:20:54Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-e9c761b1d6374d5b84c36d410f4e74a22022-12-21T19:02:36ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0189e7553410.1371/journal.pone.0075534Cj1411c encodes for a cytochrome P450 involved in Campylobacter jejuni 81-176 pathogenicity.Luis A J AlvarezBilly BourkeGratiela PircalabioruAtanas Y GeorgievUlla G KnausSimon DaffNicolae CorcionivoschiCytochrome P450s are b-heme-containing enzymes that are able to introduce oxygen atoms into a wide variety of organic substrates. They are extremely widespread in nature having diverse functions at both biochemical and physiological level. The genome of C. jejuni 81-176 encodes a single cytochrome P450 (Cj1411c) that has no close homologues. Cj1411c is unusual in its genomic location within a cluster involved in the biosynthesis of outer surface structures. Here we show that E. coli expressed and affinity-purified C. jejuni cytochrome P450 is lipophilic, containing one equivalent Cys-ligated heme. Immunoblotting confirmed the association of cytochrome P450 with membrane fractions. A Cj1411c deletion mutant had significantly reduced ability to infect human cells and was less able to survive following exposure to human serum when compared to the wild type strain. Phenotypically following staining with Alcian blue, we show that a Cj1411c deletion mutant produces significantly less capsular polysaccharide. This study describes the first known membrane-bound bacterial cytochrome P450 and its involvement in Campylobacter virulence.http://europepmc.org/articles/PMC3784454?pdf=render |
| spellingShingle | Luis A J Alvarez Billy Bourke Gratiela Pircalabioru Atanas Y Georgiev Ulla G Knaus Simon Daff Nicolae Corcionivoschi Cj1411c encodes for a cytochrome P450 involved in Campylobacter jejuni 81-176 pathogenicity. PLoS ONE |
| title | Cj1411c encodes for a cytochrome P450 involved in Campylobacter jejuni 81-176 pathogenicity. |
| title_full | Cj1411c encodes for a cytochrome P450 involved in Campylobacter jejuni 81-176 pathogenicity. |
| title_fullStr | Cj1411c encodes for a cytochrome P450 involved in Campylobacter jejuni 81-176 pathogenicity. |
| title_full_unstemmed | Cj1411c encodes for a cytochrome P450 involved in Campylobacter jejuni 81-176 pathogenicity. |
| title_short | Cj1411c encodes for a cytochrome P450 involved in Campylobacter jejuni 81-176 pathogenicity. |
| title_sort | cj1411c encodes for a cytochrome p450 involved in campylobacter jejuni 81 176 pathogenicity |
| url | http://europepmc.org/articles/PMC3784454?pdf=render |
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