Protocol to package and concentrate adeno-associated virus serotype 8 for use in autochthonous mouse models of pancreatic cancer
Summary: We have developed an economical and rapid protocol to package and concentrate adeno-associated virus serotype 8, allowing production of high-titer virus for use in vivo within 1 week. When combined with the CRISPR-Cas9 system, this provides a straightforward method for knockout of genes of...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723000667 |
| _version_ | 1811166432572997632 |
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| author | Meilian Zhuo Jiangjiao Mao Charles J. David |
| author_facet | Meilian Zhuo Jiangjiao Mao Charles J. David |
| author_sort | Meilian Zhuo |
| collection | DOAJ |
| description | Summary: We have developed an economical and rapid protocol to package and concentrate adeno-associated virus serotype 8, allowing production of high-titer virus for use in vivo within 1 week. When combined with the CRISPR-Cas9 system, this provides a straightforward method for knockout of genes of interest in the pancreas. The method can also be used to express cDNAs in the pancreas. This method shows great potential to accelerate pancreatic cancer research in autochthonous models.For complete details on the use and execution of this protocol, please refer to Li et al. (2021).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-10T15:52:17Z |
| format | Article |
| id | doaj.art-e9c7d78dc3e54fe6a230fe6d83efac1f |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-10T15:52:17Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-e9c7d78dc3e54fe6a230fe6d83efac1f2023-02-11T04:16:17ZengElsevierSTAR Protocols2666-16672023-03-0141102108Protocol to package and concentrate adeno-associated virus serotype 8 for use in autochthonous mouse models of pancreatic cancerMeilian Zhuo0Jiangjiao Mao1Charles J. David2Tsinghua University School of Medicine, Beijing, China; Peking University-Tsinghua Center for Life Sciences, Beijing, China; Corresponding authorTsinghua University School of Medicine, Beijing, ChinaTsinghua University School of Medicine, Beijing, China; Peking University-Tsinghua Center for Life Sciences, Beijing, China; Corresponding authorSummary: We have developed an economical and rapid protocol to package and concentrate adeno-associated virus serotype 8, allowing production of high-titer virus for use in vivo within 1 week. When combined with the CRISPR-Cas9 system, this provides a straightforward method for knockout of genes of interest in the pancreas. The method can also be used to express cDNAs in the pancreas. This method shows great potential to accelerate pancreatic cancer research in autochthonous models.For complete details on the use and execution of this protocol, please refer to Li et al. (2021).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723000667Cell BiologyCell cultureFlow Cytometry/Mass CytometryCell-based AssaysCancerGenetics |
| spellingShingle | Meilian Zhuo Jiangjiao Mao Charles J. David Protocol to package and concentrate adeno-associated virus serotype 8 for use in autochthonous mouse models of pancreatic cancer STAR Protocols Cell Biology Cell culture Flow Cytometry/Mass Cytometry Cell-based Assays Cancer Genetics |
| title | Protocol to package and concentrate adeno-associated virus serotype 8 for use in autochthonous mouse models of pancreatic cancer |
| title_full | Protocol to package and concentrate adeno-associated virus serotype 8 for use in autochthonous mouse models of pancreatic cancer |
| title_fullStr | Protocol to package and concentrate adeno-associated virus serotype 8 for use in autochthonous mouse models of pancreatic cancer |
| title_full_unstemmed | Protocol to package and concentrate adeno-associated virus serotype 8 for use in autochthonous mouse models of pancreatic cancer |
| title_short | Protocol to package and concentrate adeno-associated virus serotype 8 for use in autochthonous mouse models of pancreatic cancer |
| title_sort | protocol to package and concentrate adeno associated virus serotype 8 for use in autochthonous mouse models of pancreatic cancer |
| topic | Cell Biology Cell culture Flow Cytometry/Mass Cytometry Cell-based Assays Cancer Genetics |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723000667 |
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