Protocol to package and concentrate adeno-associated virus serotype 8 for use in autochthonous mouse models of pancreatic cancer

Protocol to package and concentrate adeno-associated virus serotype 8 for use in autochthonous mouse models of pancreatic cancer

Summary: We have developed an economical and rapid protocol to package and concentrate adeno-associated virus serotype 8, allowing production of high-titer virus for use in vivo within 1 week. When combined with the CRISPR-Cas9 system, this provides a straightforward method for knockout of genes of...

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Bibliographic Details
Main Authors:	Meilian Zhuo, Jiangjiao Mao, Charles J. David
Format:	Article
Language:	English
Published:	Elsevier 2023-03-01
Series:	STAR Protocols
Subjects:	Cell Biology Cell culture Flow Cytometry/Mass Cytometry Cell-based Assays Cancer Genetics
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166723000667

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