Neuroprotective and antioxidant activities of saponins’ mixture from Astragalus glycyphylloides in a model of 6-hydroxydopamine-induced oxidative stress on isolated rat brain synaptosomes
The aim of the study was to investigate the possible neuroprotective and antioxidant activity of purified saponins’mixture (PSM), isolated from Astragalus glycyphylloides (Fabaceae), in a model of 6-hydroxydipamine (6-OHDA)-induced oxidative stress on isolated rat brain synaptosomes. Synaptosomes we...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Pensoft Publishers
2019-12-01
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| Series: | Pharmacia |
| Online Access: | https://pharmacia.pensoft.net/article/37997/download/pdf/ |
| _version_ | 1818343352227069952 |
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| author | Magdalena Kondeva-Burdina Ilina Krasteva Georgi Popov Vasil Manov |
| author_facet | Magdalena Kondeva-Burdina Ilina Krasteva Georgi Popov Vasil Manov |
| author_sort | Magdalena Kondeva-Burdina |
| collection | DOAJ |
| description | The aim of the study was to investigate the possible neuroprotective and antioxidant activity of purified saponins’mixture (PSM), isolated from Astragalus glycyphylloides (Fabaceae), in a model of 6-hydroxydipamine (6-OHDA)-induced oxidative stress on isolated rat brain synaptosomes. Synaptosomes were incubated with 3 different concentrations of PSM: 60 µg/mL; 6 µg/mL; 0.6 µg/mL. The effects of PSM were compared to those of silymarin (S), at the same concentrations. The main parameters, characterized functional and metabolic status of synaptosomes, were investigated: viability (MTT-test) and level of reduced glutathione (GSH). At isolated rat brain synaptosomes, in conditions of 6-OHDA-induced oxidative stress (150 μМ), PSM revealed statistically significant, concentration-dependent, neuroprotective and antioxidant effects, compared to those of silymarin. Effects were most prominent at concentration 60 µg/mL. These neuroprotective effects of PSM might be due to the possible activity as scavenger of reactive oxygen species (ROS), produced by p-quinone (toxic metabolite of 6-OHDA). |
| first_indexed | 2024-12-13T16:29:13Z |
| format | Article |
| id | doaj.art-e9cce53b4f084261a5bfa19411f97ca7 |
| institution | Directory Open Access Journal |
| issn | 2603-557X |
| language | English |
| last_indexed | 2024-12-13T16:29:13Z |
| publishDate | 2019-12-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | Pharmacia |
| spelling | doaj.art-e9cce53b4f084261a5bfa19411f97ca72022-12-21T23:38:32ZengPensoft PublishersPharmacia2603-557X2019-12-0166423323610.3897/pharmacia.66.e3799737997Neuroprotective and antioxidant activities of saponins’ mixture from Astragalus glycyphylloides in a model of 6-hydroxydopamine-induced oxidative stress on isolated rat brain synaptosomesMagdalena Kondeva-Burdina0Ilina Krasteva1Georgi Popov2Vasil Manov3Medical University of SofiaMedical University of SofiaUniversity of ForestryUniversity of ForestryThe aim of the study was to investigate the possible neuroprotective and antioxidant activity of purified saponins’mixture (PSM), isolated from Astragalus glycyphylloides (Fabaceae), in a model of 6-hydroxydipamine (6-OHDA)-induced oxidative stress on isolated rat brain synaptosomes. Synaptosomes were incubated with 3 different concentrations of PSM: 60 µg/mL; 6 µg/mL; 0.6 µg/mL. The effects of PSM were compared to those of silymarin (S), at the same concentrations. The main parameters, characterized functional and metabolic status of synaptosomes, were investigated: viability (MTT-test) and level of reduced glutathione (GSH). At isolated rat brain synaptosomes, in conditions of 6-OHDA-induced oxidative stress (150 μМ), PSM revealed statistically significant, concentration-dependent, neuroprotective and antioxidant effects, compared to those of silymarin. Effects were most prominent at concentration 60 µg/mL. These neuroprotective effects of PSM might be due to the possible activity as scavenger of reactive oxygen species (ROS), produced by p-quinone (toxic metabolite of 6-OHDA).https://pharmacia.pensoft.net/article/37997/download/pdf/ |
| spellingShingle | Magdalena Kondeva-Burdina Ilina Krasteva Georgi Popov Vasil Manov Neuroprotective and antioxidant activities of saponins’ mixture from Astragalus glycyphylloides in a model of 6-hydroxydopamine-induced oxidative stress on isolated rat brain synaptosomes Pharmacia |
| title | Neuroprotective and antioxidant activities of saponins’ mixture from Astragalus glycyphylloides in a model of 6-hydroxydopamine-induced oxidative stress on isolated rat brain synaptosomes |
| title_full | Neuroprotective and antioxidant activities of saponins’ mixture from Astragalus glycyphylloides in a model of 6-hydroxydopamine-induced oxidative stress on isolated rat brain synaptosomes |
| title_fullStr | Neuroprotective and antioxidant activities of saponins’ mixture from Astragalus glycyphylloides in a model of 6-hydroxydopamine-induced oxidative stress on isolated rat brain synaptosomes |
| title_full_unstemmed | Neuroprotective and antioxidant activities of saponins’ mixture from Astragalus glycyphylloides in a model of 6-hydroxydopamine-induced oxidative stress on isolated rat brain synaptosomes |
| title_short | Neuroprotective and antioxidant activities of saponins’ mixture from Astragalus glycyphylloides in a model of 6-hydroxydopamine-induced oxidative stress on isolated rat brain synaptosomes |
| title_sort | neuroprotective and antioxidant activities of saponins mixture from astragalus glycyphylloides in a model of 6 hydroxydopamine induced oxidative stress on isolated rat brain synaptosomes |
| url | https://pharmacia.pensoft.net/article/37997/download/pdf/ |
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