Comparison of the Formation of Plant–Microbial Interface in <i>Pisum sativum</i> L. and <i>Medicago truncatula</i> Gaertn. Nitrogen-Fixing Nodules
Different components of the symbiotic interface play an important role in providing positional information during rhizobial infection and nodule development: successive changes in cell morphology correspond to subsequent changes in the molecular architecture of the apoplast and the associated surfac...
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| Language: | English |
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MDPI AG
2023-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/18/13850 |
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| author | Anna V. Tsyganova Elena V. Seliverstova Viktor E. Tsyganov |
| author_facet | Anna V. Tsyganova Elena V. Seliverstova Viktor E. Tsyganov |
| author_sort | Anna V. Tsyganova |
| collection | DOAJ |
| description | Different components of the symbiotic interface play an important role in providing positional information during rhizobial infection and nodule development: successive changes in cell morphology correspond to subsequent changes in the molecular architecture of the apoplast and the associated surface structures. The localisation and distribution of pectins, xyloglucans, and cell wall proteins in symbiotic nodules of <i>Pisum sativum</i> and <i>Medicago truncatula</i> were studied using immunofluorescence and immunogold analysis in wild-type and ineffective mutant nodules. As a result, the ontogenetic changes in the symbiotic interface in the nodules of both species were described. Some differences in the patterns of distribution of cell wall polysaccharides and proteins between wild-type and mutant nodules can be explained by the activation of defence reaction or premature senescence in mutants. The absence of fucosylated xyloglucan in the cell walls in the <i>P. sativum</i> nodules, as well as its predominant accumulation in the cell walls of uninfected cells in the <i>M. truncatula</i> nodules, and the presence of the rhamnogalacturonan I (unbranched) backbone in meristematic cells in <i>P. sativum</i> can be attributed to the most striking species-specific features of the symbiotic interface. |
| first_indexed | 2024-03-10T22:40:46Z |
| format | Article |
| id | doaj.art-e9cd2a2b6b2c4a6c8990a301e1c30c43 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T22:40:46Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-e9cd2a2b6b2c4a6c8990a301e1c30c432023-11-19T11:04:03ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-09-0124181385010.3390/ijms241813850Comparison of the Formation of Plant–Microbial Interface in <i>Pisum sativum</i> L. and <i>Medicago truncatula</i> Gaertn. Nitrogen-Fixing NodulesAnna V. Tsyganova0Elena V. Seliverstova1Viktor E. Tsyganov2Laboratory of Molecular and Cell Biology, All-Russia Research Institute for Agricultural Microbiology, Saint Petersburg 196608, RussiaLaboratory of Molecular and Cell Biology, All-Russia Research Institute for Agricultural Microbiology, Saint Petersburg 196608, RussiaLaboratory of Molecular and Cell Biology, All-Russia Research Institute for Agricultural Microbiology, Saint Petersburg 196608, RussiaDifferent components of the symbiotic interface play an important role in providing positional information during rhizobial infection and nodule development: successive changes in cell morphology correspond to subsequent changes in the molecular architecture of the apoplast and the associated surface structures. The localisation and distribution of pectins, xyloglucans, and cell wall proteins in symbiotic nodules of <i>Pisum sativum</i> and <i>Medicago truncatula</i> were studied using immunofluorescence and immunogold analysis in wild-type and ineffective mutant nodules. As a result, the ontogenetic changes in the symbiotic interface in the nodules of both species were described. Some differences in the patterns of distribution of cell wall polysaccharides and proteins between wild-type and mutant nodules can be explained by the activation of defence reaction or premature senescence in mutants. The absence of fucosylated xyloglucan in the cell walls in the <i>P. sativum</i> nodules, as well as its predominant accumulation in the cell walls of uninfected cells in the <i>M. truncatula</i> nodules, and the presence of the rhamnogalacturonan I (unbranched) backbone in meristematic cells in <i>P. sativum</i> can be attributed to the most striking species-specific features of the symbiotic interface.https://www.mdpi.com/1422-0067/24/18/13850cell wallpectinxyloglucanarabinogalactan proteinextensininfection thread |
| spellingShingle | Anna V. Tsyganova Elena V. Seliverstova Viktor E. Tsyganov Comparison of the Formation of Plant–Microbial Interface in <i>Pisum sativum</i> L. and <i>Medicago truncatula</i> Gaertn. Nitrogen-Fixing Nodules International Journal of Molecular Sciences cell wall pectin xyloglucan arabinogalactan protein extensin infection thread |
| title | Comparison of the Formation of Plant–Microbial Interface in <i>Pisum sativum</i> L. and <i>Medicago truncatula</i> Gaertn. Nitrogen-Fixing Nodules |
| title_full | Comparison of the Formation of Plant–Microbial Interface in <i>Pisum sativum</i> L. and <i>Medicago truncatula</i> Gaertn. Nitrogen-Fixing Nodules |
| title_fullStr | Comparison of the Formation of Plant–Microbial Interface in <i>Pisum sativum</i> L. and <i>Medicago truncatula</i> Gaertn. Nitrogen-Fixing Nodules |
| title_full_unstemmed | Comparison of the Formation of Plant–Microbial Interface in <i>Pisum sativum</i> L. and <i>Medicago truncatula</i> Gaertn. Nitrogen-Fixing Nodules |
| title_short | Comparison of the Formation of Plant–Microbial Interface in <i>Pisum sativum</i> L. and <i>Medicago truncatula</i> Gaertn. Nitrogen-Fixing Nodules |
| title_sort | comparison of the formation of plant microbial interface in i pisum sativum i l and i medicago truncatula i gaertn nitrogen fixing nodules |
| topic | cell wall pectin xyloglucan arabinogalactan protein extensin infection thread |
| url | https://www.mdpi.com/1422-0067/24/18/13850 |
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