Comparative Transcriptome Analysis Reveals Hormone Signal Transduction and Sucrose Metabolism Related Genes Involved in the Regulation of Anther Dehiscence in Photo-Thermo-Sensitive Genic Male Sterile Wheat
Anther dehiscence is an important process to release pollen and then is a critical event in pollination. In the wheat photo-thermo-sensitive genic male sterility (PTGMS) line, pollen cannot release from anther since the anther cannot dehisce during anther dehiscence stage in a sterile condition. In...
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MDPI AG
2022-08-01
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| author | Tianbao Zhang Shaohua Yuan Zihan Liu Liqing Luo Haoyu Guo Yanmei Li Jianfang Bai Changping Zhao Liping Zhang |
| author_facet | Tianbao Zhang Shaohua Yuan Zihan Liu Liqing Luo Haoyu Guo Yanmei Li Jianfang Bai Changping Zhao Liping Zhang |
| author_sort | Tianbao Zhang |
| collection | DOAJ |
| description | Anther dehiscence is an important process to release pollen and then is a critical event in pollination. In the wheat photo-thermo-sensitive genic male sterility (PTGMS) line, pollen cannot release from anther since the anther cannot dehisce during anther dehiscence stage in a sterile condition. In this study, we carried out RNA-sequencing to analyze the transcriptome of one wheat PTGMS line BS366 during anther dehiscence under fertile and sterile conditions to explore the mechanism. We identified 6306 differentially expressed genes (DEGs). Weighted gene co-expression network analysis (WGCNA) and KEGG analysis showed that DEGs were mainly related to “hormone signal transduction pathway” and “starch and sucrose metabolism”. We identified 35 and 23 DEGs related hormone signal transduction and sucrose metabolism, respectively. Compared with conventional wheat Jing411, there were some changes in the contents of hormones, including JA, IAA, BR, ABA and GA3, and sucrose, during three anther dehiscence stages in the sterile condition in BS366. We performed qRT-PCR to verify the expression levels of some critical DEGs of the hormone signaling pathway and the starch and sucrose metabolism pathway. The results showed disparate expression patterns of the critical DEGs of the hormone signaling pathway and the starch and sucrose metabolism pathway in different conditions, suggesting these genes may be involved in the regulation of the anther dehiscence in BS366. Finally, we conducted a hypothesis model to reveal the regulation pathway of hormones and sucrose on anther dehiscence. The information provided new clues to the molecular mechanisms of anther dehiscence in wheat and improved wheat hybrid breeding. |
| first_indexed | 2024-03-09T11:49:56Z |
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| language | English |
| last_indexed | 2024-03-09T11:49:56Z |
| publishDate | 2022-08-01 |
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| series | Biomolecules |
| spelling | doaj.art-e9ce1cbc29324e2797ae23b51b494f092023-11-30T23:16:40ZengMDPI AGBiomolecules2218-273X2022-08-01128114910.3390/biom12081149Comparative Transcriptome Analysis Reveals Hormone Signal Transduction and Sucrose Metabolism Related Genes Involved in the Regulation of Anther Dehiscence in Photo-Thermo-Sensitive Genic Male Sterile WheatTianbao Zhang0Shaohua Yuan1Zihan Liu2Liqing Luo3Haoyu Guo4Yanmei Li5Jianfang Bai6Changping Zhao7Liping Zhang8Beijing Key Laboratory of Molecular Genetics in Hybrid Wheat, Institute of Hybrid Wheat Beijing Academy of Agriculture and Forestry Science, Beijing 100097, ChinaBeijing Key Laboratory of Molecular Genetics in Hybrid Wheat, Institute of Hybrid Wheat Beijing Academy of Agriculture and Forestry Science, Beijing 100097, ChinaBeijing Key Laboratory of Molecular Genetics in Hybrid Wheat, Institute of Hybrid Wheat Beijing Academy of Agriculture and Forestry Science, Beijing 100097, ChinaBeijing Key Laboratory of Molecular Genetics in Hybrid Wheat, Institute of Hybrid Wheat Beijing Academy of Agriculture and Forestry Science, Beijing 100097, ChinaBeijing Key Laboratory of Molecular Genetics in Hybrid Wheat, Institute of Hybrid Wheat Beijing Academy of Agriculture and Forestry Science, Beijing 100097, ChinaBeijing Key Laboratory of Molecular Genetics in Hybrid Wheat, Institute of Hybrid Wheat Beijing Academy of Agriculture and Forestry Science, Beijing 100097, ChinaBeijing Key Laboratory of Molecular Genetics in Hybrid Wheat, Institute of Hybrid Wheat Beijing Academy of Agriculture and Forestry Science, Beijing 100097, ChinaBeijing Key Laboratory of Molecular Genetics in Hybrid Wheat, Institute of Hybrid Wheat Beijing Academy of Agriculture and Forestry Science, Beijing 100097, ChinaBeijing Key Laboratory of Molecular Genetics in Hybrid Wheat, Institute of Hybrid Wheat Beijing Academy of Agriculture and Forestry Science, Beijing 100097, ChinaAnther dehiscence is an important process to release pollen and then is a critical event in pollination. In the wheat photo-thermo-sensitive genic male sterility (PTGMS) line, pollen cannot release from anther since the anther cannot dehisce during anther dehiscence stage in a sterile condition. In this study, we carried out RNA-sequencing to analyze the transcriptome of one wheat PTGMS line BS366 during anther dehiscence under fertile and sterile conditions to explore the mechanism. We identified 6306 differentially expressed genes (DEGs). Weighted gene co-expression network analysis (WGCNA) and KEGG analysis showed that DEGs were mainly related to “hormone signal transduction pathway” and “starch and sucrose metabolism”. We identified 35 and 23 DEGs related hormone signal transduction and sucrose metabolism, respectively. Compared with conventional wheat Jing411, there were some changes in the contents of hormones, including JA, IAA, BR, ABA and GA3, and sucrose, during three anther dehiscence stages in the sterile condition in BS366. We performed qRT-PCR to verify the expression levels of some critical DEGs of the hormone signaling pathway and the starch and sucrose metabolism pathway. The results showed disparate expression patterns of the critical DEGs of the hormone signaling pathway and the starch and sucrose metabolism pathway in different conditions, suggesting these genes may be involved in the regulation of the anther dehiscence in BS366. Finally, we conducted a hypothesis model to reveal the regulation pathway of hormones and sucrose on anther dehiscence. The information provided new clues to the molecular mechanisms of anther dehiscence in wheat and improved wheat hybrid breeding.https://www.mdpi.com/2218-273X/12/8/1149anther dehiscencehormonesucrosemale sterilitywheat |
| spellingShingle | Tianbao Zhang Shaohua Yuan Zihan Liu Liqing Luo Haoyu Guo Yanmei Li Jianfang Bai Changping Zhao Liping Zhang Comparative Transcriptome Analysis Reveals Hormone Signal Transduction and Sucrose Metabolism Related Genes Involved in the Regulation of Anther Dehiscence in Photo-Thermo-Sensitive Genic Male Sterile Wheat Biomolecules anther dehiscence hormone sucrose male sterility wheat |
| title | Comparative Transcriptome Analysis Reveals Hormone Signal Transduction and Sucrose Metabolism Related Genes Involved in the Regulation of Anther Dehiscence in Photo-Thermo-Sensitive Genic Male Sterile Wheat |
| title_full | Comparative Transcriptome Analysis Reveals Hormone Signal Transduction and Sucrose Metabolism Related Genes Involved in the Regulation of Anther Dehiscence in Photo-Thermo-Sensitive Genic Male Sterile Wheat |
| title_fullStr | Comparative Transcriptome Analysis Reveals Hormone Signal Transduction and Sucrose Metabolism Related Genes Involved in the Regulation of Anther Dehiscence in Photo-Thermo-Sensitive Genic Male Sterile Wheat |
| title_full_unstemmed | Comparative Transcriptome Analysis Reveals Hormone Signal Transduction and Sucrose Metabolism Related Genes Involved in the Regulation of Anther Dehiscence in Photo-Thermo-Sensitive Genic Male Sterile Wheat |
| title_short | Comparative Transcriptome Analysis Reveals Hormone Signal Transduction and Sucrose Metabolism Related Genes Involved in the Regulation of Anther Dehiscence in Photo-Thermo-Sensitive Genic Male Sterile Wheat |
| title_sort | comparative transcriptome analysis reveals hormone signal transduction and sucrose metabolism related genes involved in the regulation of anther dehiscence in photo thermo sensitive genic male sterile wheat |
| topic | anther dehiscence hormone sucrose male sterility wheat |
| url | https://www.mdpi.com/2218-273X/12/8/1149 |
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