A Ribosomal Protein AgRPS3aE from Halophilic Aspergillus glaucus Confers Salt Tolerance in Heterologous Organisms
High salt in soils is one of the abiotic stresses that significantly reduces crop yield, although saline lands are considered potential resources arable for agriculture. Currently, genetic engineering for enhancing salt tolerance is being tested as an efficient and viable strategy for crop improveme...
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MDPI AG
2015-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | http://www.mdpi.com/1422-0067/16/2/3058 |
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| author | Xilong Liang Yiling Liu Lixia Xie Xiaodan Liu Yi Wei Xiaoyang Zhou Shihong Zhang |
| author_facet | Xilong Liang Yiling Liu Lixia Xie Xiaodan Liu Yi Wei Xiaoyang Zhou Shihong Zhang |
| author_sort | Xilong Liang |
| collection | DOAJ |
| description | High salt in soils is one of the abiotic stresses that significantly reduces crop yield, although saline lands are considered potential resources arable for agriculture. Currently, genetic engineering for enhancing salt tolerance is being tested as an efficient and viable strategy for crop improvement. We previously characterized a large subunit of the ribosomal protein RPL44, which is involved in osmotic stress in the extremely halophilic fungus Aspergillus glaucus. Here, we screened another ribosomal protein (AgRPS3aE) that also produced high-salt tolerance in yeast. Bioinformatics analysis indicated that AgRPS3aE encodes a 29.2 kDa small subunit of a ribosomal protein belonging to the RPS3Ae family in eukaryotes. To further confirm its protective function against salinity, we expressed AgRPS3aE in three heterologous systems, the filamentous fungus Magnaporthe oryzae and two model plants Arabidopsis and tobacco. Overexpression of AgRPS3aE in all tested transformants significantly alleviated stress symptoms compared with controls, suggesting that AgRPS3aE functions not only in fungi but also in plants. Considering that ribosomal proteins are housekeeping components in organisms from prokaryotes to eukaryotes, we propose that AgRPS3aE is one of the optimal genes for improving high-salt tolerance in crops. |
| first_indexed | 2024-04-12T20:37:41Z |
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| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-12T20:37:41Z |
| publishDate | 2015-01-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-e9d0903f6e0d482c858751f7e5436f912022-12-22T03:17:32ZengMDPI AGInternational Journal of Molecular Sciences1422-00672015-01-011623058307010.3390/ijms16023058ijms16023058A Ribosomal Protein AgRPS3aE from Halophilic Aspergillus glaucus Confers Salt Tolerance in Heterologous OrganismsXilong Liang0Yiling Liu1Lixia Xie2Xiaodan Liu3Yi Wei4Xiaoyang Zhou5Shihong Zhang6College of Plant Sciences, Jilin University, Changchun 130062, ChinaCollege of Plant Sciences, Jilin University, Changchun 130062, ChinaCollege of Plant Sciences, Jilin University, Changchun 130062, ChinaCollege of Plant Sciences, Jilin University, Changchun 130062, ChinaCollege of Plant Sciences, Jilin University, Changchun 130062, ChinaCollege of Plant Sciences, Jilin University, Changchun 130062, ChinaCollege of Plant Sciences, Jilin University, Changchun 130062, ChinaHigh salt in soils is one of the abiotic stresses that significantly reduces crop yield, although saline lands are considered potential resources arable for agriculture. Currently, genetic engineering for enhancing salt tolerance is being tested as an efficient and viable strategy for crop improvement. We previously characterized a large subunit of the ribosomal protein RPL44, which is involved in osmotic stress in the extremely halophilic fungus Aspergillus glaucus. Here, we screened another ribosomal protein (AgRPS3aE) that also produced high-salt tolerance in yeast. Bioinformatics analysis indicated that AgRPS3aE encodes a 29.2 kDa small subunit of a ribosomal protein belonging to the RPS3Ae family in eukaryotes. To further confirm its protective function against salinity, we expressed AgRPS3aE in three heterologous systems, the filamentous fungus Magnaporthe oryzae and two model plants Arabidopsis and tobacco. Overexpression of AgRPS3aE in all tested transformants significantly alleviated stress symptoms compared with controls, suggesting that AgRPS3aE functions not only in fungi but also in plants. Considering that ribosomal proteins are housekeeping components in organisms from prokaryotes to eukaryotes, we propose that AgRPS3aE is one of the optimal genes for improving high-salt tolerance in crops.http://www.mdpi.com/1422-0067/16/2/3058Aspergillus glaucusribosomal protein AgRPS3aEsalt toleranceexpression in hetero-organisms |
| spellingShingle | Xilong Liang Yiling Liu Lixia Xie Xiaodan Liu Yi Wei Xiaoyang Zhou Shihong Zhang A Ribosomal Protein AgRPS3aE from Halophilic Aspergillus glaucus Confers Salt Tolerance in Heterologous Organisms International Journal of Molecular Sciences Aspergillus glaucus ribosomal protein AgRPS3aE salt tolerance expression in hetero-organisms |
| title | A Ribosomal Protein AgRPS3aE from Halophilic Aspergillus glaucus Confers Salt Tolerance in Heterologous Organisms |
| title_full | A Ribosomal Protein AgRPS3aE from Halophilic Aspergillus glaucus Confers Salt Tolerance in Heterologous Organisms |
| title_fullStr | A Ribosomal Protein AgRPS3aE from Halophilic Aspergillus glaucus Confers Salt Tolerance in Heterologous Organisms |
| title_full_unstemmed | A Ribosomal Protein AgRPS3aE from Halophilic Aspergillus glaucus Confers Salt Tolerance in Heterologous Organisms |
| title_short | A Ribosomal Protein AgRPS3aE from Halophilic Aspergillus glaucus Confers Salt Tolerance in Heterologous Organisms |
| title_sort | ribosomal protein agrps3ae from halophilic aspergillus glaucus confers salt tolerance in heterologous organisms |
| topic | Aspergillus glaucus ribosomal protein AgRPS3aE salt tolerance expression in hetero-organisms |
| url | http://www.mdpi.com/1422-0067/16/2/3058 |
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