<i>Propionibacteriaium acidipropionici</i> CP 88 Dose Alters In Vivo and In Vitro Ruminal Fermentation Characteristics
Twelve 5-year-old beef steers, with an average weight of 2000 lbs., fitted with rumen canulae were used in a 4 × 4 incomplete Latin square design to examine the impact of the direct fed microbial Propionibacterium acidipropionici CP 88 (PA) on rumen fermentation characteristics, in vitro CH<sub&g...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-11-01
|
| Series: | Ruminants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2673-933X/2/4/31 |
| _version_ | 1797614479270215680 |
|---|---|
| author | Jonah R. Levenson Logan Thompson Roderick Gonzalez-Murray Ryan J. Gifford Meghan P. Thorndyke Octavio Guimaraes Huey Yi Loh Briana V. Tangredi Harrison Hallmark Richard Goodall John J. Wagner Terry E. Engle |
| author_facet | Jonah R. Levenson Logan Thompson Roderick Gonzalez-Murray Ryan J. Gifford Meghan P. Thorndyke Octavio Guimaraes Huey Yi Loh Briana V. Tangredi Harrison Hallmark Richard Goodall John J. Wagner Terry E. Engle |
| author_sort | Jonah R. Levenson |
| collection | DOAJ |
| description | Twelve 5-year-old beef steers, with an average weight of 2000 lbs., fitted with rumen canulae were used in a 4 × 4 incomplete Latin square design to examine the impact of the direct fed microbial Propionibacterium acidipropionici CP 88 (PA) on rumen fermentation characteristics, in vitro CH<sub>4</sub>, CO<sub>2</sub>, and N<sub>2</sub> production, and in vivo CH<sub>4</sub> and CO<sub>2</sub> production. All steers were housed in the same pen equipped with eight GrowSafe feeding stations to monitor individual animal feed intake and one GreenFeed System to estimate individual animal CH<sub>4</sub> and CO<sub>2</sub> production. Steers were fed a corn-silage-based diet throughout the experiment. Treatments consisted of PA administered at: (1) control (0.0); (2) 1.0 × 10<sup>8</sup>; (3) 1.0 × 10<sup>9</sup>; and (4) 1.0 × 10<sup>10</sup> cfu∙animal<sup>−1</sup>∙day (d)<sup>−1</sup>. Treatments were administered directly into the rumen as a single bolus dose daily. On day 7 and 14 of each period, rumen fluid was collected from each steer 2 h post treatment administration for VFA analysis and in vitro DM digestibility determination. Following a 14 d washout period, animal treatments were switched and the experiment repeated until the 4 × 4 Latin square was complete. In vivo propionic acid molar proportions and total VFA concentrations were greater (<i>p</i> < 0.05) in steers receiving PA when compared with controls. All other in vivo rumen fermentation characteristics were similar across treatments. In vitro DM disappearance (<i>p</i> < 0.05) and total VFA (<i>p</i> < 0.05) were greater and CH<sub>4</sub> lesser (<i>p</i> < 0.04) in fermentation vessels incubated with rumen fluid from animals receiving PA when compared with controls. Dry matter disappearance (<i>p</i> < 0.03) and propionic acid molar proportions increased (<i>p</i> < 0.04) linearly as the dose of PA increased. In vitro total VFA tended (<i>p</i> < 0.08) to increase linearly and CH<sub>4</sub> production per unit of DM digested tended (<i>p</i> < 0.09) to decrease quadratically in response to PA dose. All other in vitro rumen fermentation characteristics were similar across treatments. These data indicate that PA impacts in vivo and in vitro rumen fermentation characteristics. |
| first_indexed | 2024-03-11T07:12:01Z |
| format | Article |
| id | doaj.art-e9d3d1f79751410e97e56dd5d7bfa1c0 |
| institution | Directory Open Access Journal |
| issn | 2673-933X |
| language | English |
| last_indexed | 2024-03-11T07:12:01Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Ruminants |
| spelling | doaj.art-e9d3d1f79751410e97e56dd5d7bfa1c02023-11-17T08:33:40ZengMDPI AGRuminants2673-933X2022-11-012444845510.3390/ruminants2040031<i>Propionibacteriaium acidipropionici</i> CP 88 Dose Alters In Vivo and In Vitro Ruminal Fermentation CharacteristicsJonah R. Levenson0Logan Thompson1Roderick Gonzalez-Murray2Ryan J. Gifford3Meghan P. Thorndyke4Octavio Guimaraes5Huey Yi Loh6Briana V. Tangredi7Harrison Hallmark8Richard Goodall9John J. Wagner10Terry E. Engle11Department of Animal Science, Colorado State University, Fort Collins, CO 80523, USADepartment of Animal Science, Colorado State University, Fort Collins, CO 80523, USADepartment of Animal Science, Colorado State University, Fort Collins, CO 80523, USADepartment of Animal Science, Colorado State University, Fort Collins, CO 80523, USADepartment of Animal Science, Colorado State University, Fort Collins, CO 80523, USADepartment of Animal Science, Colorado State University, Fort Collins, CO 80523, USADepartment of Animal Science, Colorado State University, Fort Collins, CO 80523, USADepartment of Animal Science, Colorado State University, Fort Collins, CO 80523, USADepartment of Animal Science, Colorado State University, Fort Collins, CO 80523, USAMicrobios Inc., Houston, TX 77063, USADepartment of Animal Science, Colorado State University, Fort Collins, CO 80523, USADepartment of Animal Science, Colorado State University, Fort Collins, CO 80523, USATwelve 5-year-old beef steers, with an average weight of 2000 lbs., fitted with rumen canulae were used in a 4 × 4 incomplete Latin square design to examine the impact of the direct fed microbial Propionibacterium acidipropionici CP 88 (PA) on rumen fermentation characteristics, in vitro CH<sub>4</sub>, CO<sub>2</sub>, and N<sub>2</sub> production, and in vivo CH<sub>4</sub> and CO<sub>2</sub> production. All steers were housed in the same pen equipped with eight GrowSafe feeding stations to monitor individual animal feed intake and one GreenFeed System to estimate individual animal CH<sub>4</sub> and CO<sub>2</sub> production. Steers were fed a corn-silage-based diet throughout the experiment. Treatments consisted of PA administered at: (1) control (0.0); (2) 1.0 × 10<sup>8</sup>; (3) 1.0 × 10<sup>9</sup>; and (4) 1.0 × 10<sup>10</sup> cfu∙animal<sup>−1</sup>∙day (d)<sup>−1</sup>. Treatments were administered directly into the rumen as a single bolus dose daily. On day 7 and 14 of each period, rumen fluid was collected from each steer 2 h post treatment administration for VFA analysis and in vitro DM digestibility determination. Following a 14 d washout period, animal treatments were switched and the experiment repeated until the 4 × 4 Latin square was complete. In vivo propionic acid molar proportions and total VFA concentrations were greater (<i>p</i> < 0.05) in steers receiving PA when compared with controls. All other in vivo rumen fermentation characteristics were similar across treatments. In vitro DM disappearance (<i>p</i> < 0.05) and total VFA (<i>p</i> < 0.05) were greater and CH<sub>4</sub> lesser (<i>p</i> < 0.04) in fermentation vessels incubated with rumen fluid from animals receiving PA when compared with controls. Dry matter disappearance (<i>p</i> < 0.03) and propionic acid molar proportions increased (<i>p</i> < 0.04) linearly as the dose of PA increased. In vitro total VFA tended (<i>p</i> < 0.08) to increase linearly and CH<sub>4</sub> production per unit of DM digested tended (<i>p</i> < 0.09) to decrease quadratically in response to PA dose. All other in vitro rumen fermentation characteristics were similar across treatments. These data indicate that PA impacts in vivo and in vitro rumen fermentation characteristics.https://www.mdpi.com/2673-933X/2/4/31beef cattledirect fed microbialvolatile fatty acidsmethane |
| spellingShingle | Jonah R. Levenson Logan Thompson Roderick Gonzalez-Murray Ryan J. Gifford Meghan P. Thorndyke Octavio Guimaraes Huey Yi Loh Briana V. Tangredi Harrison Hallmark Richard Goodall John J. Wagner Terry E. Engle <i>Propionibacteriaium acidipropionici</i> CP 88 Dose Alters In Vivo and In Vitro Ruminal Fermentation Characteristics Ruminants beef cattle direct fed microbial volatile fatty acids methane |
| title | <i>Propionibacteriaium acidipropionici</i> CP 88 Dose Alters In Vivo and In Vitro Ruminal Fermentation Characteristics |
| title_full | <i>Propionibacteriaium acidipropionici</i> CP 88 Dose Alters In Vivo and In Vitro Ruminal Fermentation Characteristics |
| title_fullStr | <i>Propionibacteriaium acidipropionici</i> CP 88 Dose Alters In Vivo and In Vitro Ruminal Fermentation Characteristics |
| title_full_unstemmed | <i>Propionibacteriaium acidipropionici</i> CP 88 Dose Alters In Vivo and In Vitro Ruminal Fermentation Characteristics |
| title_short | <i>Propionibacteriaium acidipropionici</i> CP 88 Dose Alters In Vivo and In Vitro Ruminal Fermentation Characteristics |
| title_sort | i propionibacteriaium acidipropionici i cp 88 dose alters in vivo and in vitro ruminal fermentation characteristics |
| topic | beef cattle direct fed microbial volatile fatty acids methane |
| url | https://www.mdpi.com/2673-933X/2/4/31 |
| work_keys_str_mv | AT jonahrlevenson ipropionibacteriaiumacidipropioniciicp88dosealtersinvivoandinvitroruminalfermentationcharacteristics AT loganthompson ipropionibacteriaiumacidipropioniciicp88dosealtersinvivoandinvitroruminalfermentationcharacteristics AT roderickgonzalezmurray ipropionibacteriaiumacidipropioniciicp88dosealtersinvivoandinvitroruminalfermentationcharacteristics AT ryanjgifford ipropionibacteriaiumacidipropioniciicp88dosealtersinvivoandinvitroruminalfermentationcharacteristics AT meghanpthorndyke ipropionibacteriaiumacidipropioniciicp88dosealtersinvivoandinvitroruminalfermentationcharacteristics AT octavioguimaraes ipropionibacteriaiumacidipropioniciicp88dosealtersinvivoandinvitroruminalfermentationcharacteristics AT hueyyiloh ipropionibacteriaiumacidipropioniciicp88dosealtersinvivoandinvitroruminalfermentationcharacteristics AT brianavtangredi ipropionibacteriaiumacidipropioniciicp88dosealtersinvivoandinvitroruminalfermentationcharacteristics AT harrisonhallmark ipropionibacteriaiumacidipropioniciicp88dosealtersinvivoandinvitroruminalfermentationcharacteristics AT richardgoodall ipropionibacteriaiumacidipropioniciicp88dosealtersinvivoandinvitroruminalfermentationcharacteristics AT johnjwagner ipropionibacteriaiumacidipropioniciicp88dosealtersinvivoandinvitroruminalfermentationcharacteristics AT terryeengle ipropionibacteriaiumacidipropioniciicp88dosealtersinvivoandinvitroruminalfermentationcharacteristics |